Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,157,770	A→G	T41A (ACT→GCT)	yqhG →	DUF3828 family putative periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,157,770	0	A	G	100.0%	97.7 / NA	28	T41A (ACT→GCT)	yqhG	DUF3828 family putative periplasmic protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/10); total (18/10)

TGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTGG  >  NC_000913/3157640‑3157915

tGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGa                                                                                                                                >  2:713834/1‑150 (MQ=255)

tGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGa                                                                                                                                >  2:387849/1‑150 (MQ=255)

 gAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGaa                                                                                                                               >  1:463932/1‑150 (MQ=255)

      aCAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCgg                                                                                                                          >  1:613342/1‑150 (MQ=255)

            gAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCg                                                                                                                    <  2:608137/150‑1 (MQ=255)

                   ataCTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATaa                                                                                                            <  1:412725/151‑1 (MQ=255)

                   ataCTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATa                                                                                                             >  2:109794/1‑150 (MQ=255)

                         cTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTg                                                                                                      >  2:456411/1‑151 (MQ=255)

                                   cagcCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCa                                                                                            <  1:109796/151‑1 (MQ=255)

                                    agcCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCaa                                                                                           <  2:258432/151‑1 (MQ=255)

                                          ggCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAgg                                                                                       <  2:575887/149‑1 (MQ=255)

                                                      cGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAAc                                                                         <  2:578038/151‑1 (MQ=255)

                                                         acacGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGAc                                                                      <  2:129839/151‑1 (MQ=255)

                                                                             aGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCa                                                   >  2:93955/1‑150 (MQ=255)

                                                                               aCCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAat                                                 >  2:502790/1‑150 (MQ=255)

                                                                                ccGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAatat                                               >  1:544254/1‑151 (MQ=255)

                                                                                 cGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAtt                                              >  1:283030/1‑151 (MQ=255)

                                                                                       acaaacaGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATtggctgg                                        <  2:430229/151‑1 (MQ=255)

                                                                                            caGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGa                                   <  2:493836/151‑1 (MQ=255)

                                                                                                 cGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATg                              >  1:477970/1‑151 (MQ=255)

                                                                                                     atatTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATcc                          >  1:336494/1‑151 (MQ=255)

                                                                                                        tttATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGt                       >  1:753526/1‑151 (MQ=255)

                                                                                                             cAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTtgtg                  >  2:568776/1‑151 (MQ=255)

                                                                                                                   tataAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTc            >  2:196599/1‑151 (MQ=255)

                                                                                                                    ataAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTc            >  1:177812/1‑150 (MQ=255)

                                                                                                                        aTCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTCCGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGAt       <  1:497685/151‑1 (MQ=255)

                                                                                                                           aGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGAtttt    >  2:511602/1‑151 (MQ=255)

                                                                                                                             aTGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTgg  >  2:261962/1‑151 (MQ=255)

TGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTGG  >  NC_000913/3157640‑3157915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A