Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,161,711	A→G	V320A (GTG→GCG)	ftsP ←	septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,161,711	0	A	G	100.0%	168.9 / NA	47	V320A (GTG→GCG)	ftsP	septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/25); total (22/25)

ACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCGCGGCGCTCGCCCGGTGCCAG  >  NC_000913/3161565‑3161853

acaGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGcc                                                                                                                                              >  1:489275/1‑149 (MQ=255)

 caGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGTCAAAAGGCCGGTTGGGCGCAGCGTTAGCGCCAgg                                                                                                                                           >  2:69388/1‑151 (MQ=255)

 caGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAgg                                                                                                                                           >  2:210720/1‑151 (MQ=255)

  aGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAggg                                                                                                                                          <  1:476660/151‑1 (MQ=255)

      gTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAg                                                                                                                                       <  1:702153/150‑1 (MQ=255)

        ccATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGaa                                                                                                                                     <  1:545335/150‑1 (MQ=255)

                aCCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAAt                                                                                                                             <  1:557656/150‑1 (MQ=255)

                 cccGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATAc                                                                                                                           >  1:362189/1‑151 (MQ=255)

                  ccGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACt                                                                                                                          >  2:490253/1‑151 (MQ=255)

                   cGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTg                                                                                                                         >  2:665432/1‑151 (MQ=255)

                           caCCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTc                                                                                                                 <  2:18550/151‑1 (MQ=255)

                             cccAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCaa                                                                                                               <  1:668785/151‑1 (MQ=255)

                                 gACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAaagaa                                                                                                           <  2:468470/151‑1 (MQ=255)

                                     gATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGc                                                                                                         <  1:191196/149‑1 (MQ=255)

                                        atCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGa                                                                                                    <  2:169181/151‑1 (MQ=255)

                                         tCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGa                                                                                                    >  2:159261/1‑150 (MQ=255)

                                          cgcgACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAAt                                                                                                  <  1:756271/151‑1 (MQ=255)

                                                tGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGGAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATc                                                                                            >  1:541332/1‑151 (MQ=255)

                                                 gCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATca                                                                                           <  1:222647/151‑1 (MQ=255)

                                                    aaTTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATcaac                                                                                         >  1:521194/1‑150 (MQ=255)

                                                       tGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATg                                                                                     >  2:487199/1‑151 (MQ=255)

                                                              ccAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTcgccg                                                                              <  2:22116/151‑1 (MQ=255)

                                                                  ccATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTc                                                                          >  2:428003/1‑151 (MQ=255)

                                                                     tGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGcc                                                                       >  1:95475/1‑151 (MQ=255)

                                                                                ggCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCg                                                            >  1:390558/1‑151 (MQ=255)

                                                                                    gcaAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGacac                                                        >  1:502003/1‑151 (MQ=255)

                                                                                       aGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTc                                                     <  2:520863/151‑1 (MQ=255)

                                                                                                gAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGtt                                            >  2:604845/1‑151 (MQ=255)

                                                                                                 aaGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGt                                             >  2:739672/1‑149 (MQ=255)

                                                                                                       gtctgtGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCat                                      <  2:169154/150‑1 (MQ=255)

                                                                                                        tctgtGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCatat                                    >  1:660229/1‑151 (MQ=255)

                                                                                                          tgtgACCAGCGGCAGAAGGCCGGTTGGGCGCGGCGTTAGCGCCAGGGTAGAAGCCAGAACACTGGATGGCTCAAAGAGGCTACGAATACGATCAACAATGGTCGCGGCGGCGCCACAGGTGATCGACGCTTTATCGCCGTTTCTCata                                     <  2:86665/148‑1 (MQ=255)

                                                                                                           gtgACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCatat                                    <  1:455926/148‑1 (MQ=255)

                                                                                                           gtgACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATcc                                  <  1:314175/150‑1 (MQ=255)

                                                                                                           gtgACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATcc                                  <  1:23413/150‑1 (MQ=255)

                                                                                                           gtgACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATc                                   <  2:710747/149‑1 (MQ=255)

                                                                                                                   cGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGaa                           <  2:31082/149‑1 (MQ=255)

                                                                                                                   cGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAAtc                         <  2:374957/151‑1 (MQ=255)

                                                                                                                        gAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTcgcg                    >  1:411876/1‑151 (MQ=255)

                                                                                                                         aaGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCgcgg                   >  2:227371/1‑151 (MQ=255)

                                                                                                                             ccGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCtt                                                                           <  2:372663/91‑1 (MQ=255)

                                                                                                                             ccGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCtt                                                                           >  1:372679/1‑91 (MQ=255)

                                                                                                                                gTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCGCGGCGCTCGc            >  2:655672/1‑151 (MQ=255)

                                                                                                                                     gcgcAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCGCGGCGCTCGCCCGGt       <  1:159268/151‑1 (MQ=255)

                                                                                                                                      cgcAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCGCGGCGCTCGCCCGGTg      <  1:339196/151‑1 (MQ=255)

                                                                                                                                       gcAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCGCGGCGCTCGCCCGGTGc     >  2:433882/1‑151 (MQ=255)

                                                                                                                                          gCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCGCGGCGCTCGCCCGGTGCCAg  <  2:327899/151‑1 (MQ=255)

ACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCGCGGCGCTCGCCCGGTGCCAG  >  NC_000913/3161565‑3161853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A