Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,162,669	T→C	M1M (ATG→GTG) †	ftsP ←	septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,162,669	0	T	C	100.0%	167.4 / NA	43	M1M (ATG→GTG) †	ftsP	septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/25); total (18/25)

AAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGACTTCTTTATCGAGCTCGGCGATTTTTTGTTCCATTATCG  >  NC_000913/3162523‑3162814

aaaCAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACAttt                                                                                                                                               >  2:75669/1‑151 (MQ=255)

aaaCAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACAttt                                                                                                                                               >  1:58287/1‑151 (MQ=255)

        gTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATg                                                                                                                                       >  1:512863/1‑151 (MQ=255)

               aCGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACt                                                                                                                                <  1:396710/151‑1 (MQ=255)

                cGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTg                                                                                                                               <  2:130989/151‑1 (MQ=255)

                    gagaTTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCt                                                                                                                            >  1:472753/1‑150 (MQ=255)

                     agaTTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTaa                                                                                                                          <  2:19365/151‑1 (MQ=255)

                        ttCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGt                                                                                                                       >  1:294566/1‑151 (MQ=255)

                           aaGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAAt                                                                                                                    <  2:252657/151‑1 (MQ=255)

                                      gAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAgg                                                                                                          >  1:658294/1‑150 (MQ=255)

                                        aCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGaa                                                                                                        >  1:253026/1‑150 (MQ=255)

                                                     gCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCaa                                                                                          <  2:255382/151‑1 (MQ=255)

                                                          tGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAAt                                                                                      <  1:594334/150‑1 (MQ=255)

                                                          tGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAAt                                                                                      <  2:452807/150‑1 (MQ=255)

                                                                gCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGAtt                                                                               <  2:133385/151‑1 (MQ=255)

                                                                  tGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGc                                                                             >  1:499296/1‑151 (MQ=255)

                                                                    cgcTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGcca                                                                           <  2:20806/151‑2 (MQ=255)

                                                                    cgcTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCg                                                                           <  2:67157/151‑1 (MQ=255)

                                                                     gcTGGCCATCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGa                                                                          <  1:587328/151‑1 (MQ=255)

                                                                               aGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACttt                                                                <  1:273173/151‑1 (MQ=255)

                                                                                    aaCAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCcgg                                                           >  2:48373/1‑151 (MQ=255)

                                                                                        gcgcCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCggcggc                                                       <  2:534195/151‑1 (MQ=255)

                                                                                           ccTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTc                                                    <  2:511240/151‑1 (MQ=255)

                                                                                               cacaAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGt                                                >  2:549659/1‑151 (MQ=255)

                                                                                                 caAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTc                                              >  1:160465/1‑151 (MQ=255)

                                                                                                    aGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGc                                           <  1:547225/151‑1 (MQ=255)

                                                                                                       gCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGAc                                        >  1:37597/1‑151 (MQ=255)

                                                                                                         aaTCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGActt                                      <  1:594349/151‑1 (MQ=255)

                                                                                                           tCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGActtct                                    >  2:585216/1‑151 (MQ=255)

                                                                                                             cccGATGCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGACTTCttt                                  >  2:492028/1‑151 (MQ=255)

                                                                                                                   gCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGACTTCTTTATCGa                             <  1:101678/150‑1 (MQ=255)

                                                                                                                   gCCTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGACTTCTTTATCGa                             <  1:25454/150‑1 (MQ=255)

                                                                                                                    ccTGAATGAACTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGACTTCTTTATCGAGc                           <  1:667189/151‑1 (MQ=255)

                                                                                                                       gaatgaaCTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGACTTCTTTATCGAGCta                         <  2:608675/150‑2 (MQ=255)

                                                                                                                       gaatgaaCTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGACTTCTTTATCGAGCTc                         <  2:679294/150‑1 (MQ=255)

                                                                                                                        aatgaaCTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGACTTCTTTATCGAGCTCgg                       >  1:205593/1‑151 (MQ=255)

                                                                                                                           gaaCTGACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGACTTCTTTATCGAGCTCGGCGa                    <  1:223221/151‑1 (MQ=255)

                                                                                                                                gACGCCGACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGACTTCTTTATCGAGCTCGGCGAtttt                <  2:408856/150‑1 (MQ=255)

                                                                                                                                      gACTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGACTTCGCGTTCTGCGACTTCTTTATCGAGCTCGGCGATTTTTTGTTcc         >  2:193341/1‑151 (MQ=255)

                                                                                                                                       aCTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGACTTCTTTATCGAGCTCGGCGATTTTTTGTTCCa        <  1:143776/151‑1 (MQ=255)

                                                                                                                                        cTGAGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGACTTCTTTATCGAGCTCGGCGATTTTTTGTTCCAt       >  2:479384/1‑151 (MQ=255)

                                                                                                                                           aGTGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGACTTCTTTATCGAGCTCGGCGATTTTTTGTTCCATTAt    <  2:447738/151‑1 (MQ=255)

                                                                                                                                             tGACACATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGACTTCTTTATCGAGCTCGGCGATTTTTTGTTCCATTATCg  >  1:543809/1‑151 (MQ=255)

AAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGCTAAAGTAATACCGTGCAGGAAAGTGTTCCCCGCAAAAATACAGATTGCCGACTTAAACTTTTCCGGCGGCTTCGCGTTCTGCGACTTCTTTATCGAGCTCGGCGATTTTTTGTTCCATTATCG  >  NC_000913/3162523‑3162814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A