Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,390,865	T→C	A388A (GCA→GCG)	tldD ←	putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,390,865	0	T	C	100.0%	150.6 / NA	42	A388A (GCA→GCG)	tldD	putative peptidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (30/12); total (30/12)

ATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGTGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCATCCCCATCAAACGC  >  NC_000913/3390725‑3391012

aTGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATAc                                                                                                                                           >  1:564608/1‑151 (MQ=255)

 tGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATAcc                                                                                                                                          >  1:655064/1‑151 (MQ=255)

   gTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGt                                                                                                                                        >  1:522233/1‑151 (MQ=255)

             cGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACgg                                                                                                                               >  1:730660/1‑150 (MQ=255)

             cGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACgg                                                                                                                               >  2:170639/1‑150 (MQ=255)

               gAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGAtt                                                                                                                            <  1:166891/151‑1 (MQ=255)

                  ccAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCa                                                                                                                          >  2:64311/1‑150 (MQ=255)

                   cAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCaat                                                                                                                        <  1:749371/151‑1 (MQ=255)

                      tCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCaataat                                                                                                                     >  1:411612/1‑151 (MQ=255)

                      tCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCaataat                                                                                                                     >  1:97841/1‑151 (MQ=255)

                           gTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCt                                                                                                                >  2:672663/1‑151 (MQ=255)

                             tGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTg                                                                                                               <  1:64312/150‑1 (MQ=255)

                             tGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTg                                                                                                               <  1:241162/150‑1 (MQ=255)

                              gcgcCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCg                                                                                                             <  2:584140/151‑1 (MQ=255)

                                  cTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGt                                                                                                         >  2:728099/1‑151 (MQ=255)

                                        ccGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGAttt                                                                                                   >  2:389804/1‑151 (MQ=255)

                                         cGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGa                                                                                                      <  1:671657/147‑1 (MQ=255)

                                              tCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCgg                                                                                             >  1:581581/1‑151 (MQ=255)

                                                gTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGgc                                                                                            >  1:717534/1‑150 (MQ=255)

                                                       tACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCatat                                                                                     >  2:616790/1‑150 (MQ=255)

                                                         ccGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAgg                                                                                  >  2:147035/1‑151 (MQ=255)

                                                               tCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTgg                                                                            >  1:550866/1‑151 (MQ=255)

                                                                cAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGt                                                                           <  1:133817/151‑1 (MQ=255)

                                                                cAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGt                                                                           >  1:518275/1‑151 (MQ=255)

                                                                           gCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGc                                                                 >  1:271661/1‑150 (MQ=255)

                                                                           gCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGc                                                                 >  2:484474/1‑150 (MQ=255)

                                                                             ttCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATg                                                              >  1:86299/1‑151 (MQ=255)

                                                                               cTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATggg                                                            >  1:349853/1‑151 (MQ=255)

                                                                                          aaaaCGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGt                                                 <  1:381904/151‑1 (MQ=255)

                                                                                                   ttGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCa                                         <  2:161361/150‑1 (MQ=255)

                                                                                                        ggAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCg                                    >  2:703624/1‑150 (MQ=255)

                                                                                                        ggAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCg                                    >  1:326772/1‑150 (MQ=255)

                                                                                                                tatCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGcc                           >  1:183224/1‑151 (MQ=255)

                                                                                                                  tCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGcc                           >  1:460030/1‑149 (MQ=255)

                                                                                                                       cTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGc                    >  2:332466/1‑151 (MQ=255)

                                                                                                                           ccGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTc                 >  2:418823/1‑150 (MQ=255)

                                                                                                                            cGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCAt               <  1:417573/151‑1 (MQ=255)

                                                                                                                                  caAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCATCCCCAt         >  1:134573/1‑151 (MQ=255)

                                                                                                                                   aaaGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCATCCCCATc        >  2:394284/1‑151 (MQ=255)

                                                                                                                                        tCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCATCCCCATCAAAc    <  2:326759/150‑1 (MQ=255)

                                                                                                                                         cGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCATCCCCATCAAAcgc  >  1:531101/1‑151 (MQ=255)

                                                                                                                                         cGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCATCCCCATCAAAcg   <  2:662815/150‑1 (MQ=255)

ATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGTGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCATCCCCATCAAACGC  >  NC_000913/3390725‑3391012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A