Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I1 R1 163 55.0 1534755 98.6% 1513268 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,505,488 A→G V178A (GTG→GCG)  yhfS ← FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,505,4880AG100.0% 148.8 / NA 41V178A (GTG→GCG) yhfSFNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/16);  total (25/16)

CTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTC  >  NC_000913/3505343‑3505627
                                                                                                                                                 |                                                                                                                                           
cTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTtc                                                                                                                                        >  2:331142/1‑151 (MQ=255)
  ggCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTtcat                                                                                                                                      <  2:534676/151‑1 (MQ=255)
     tACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCAc                                                                                                                                   >  2:624033/1‑151 (MQ=255)
      aCCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTtcatca                                                                                                                                    <  1:84668/149‑1 (MQ=255)
          ccgGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCAt                                                                                                                              <  1:597043/151‑1 (MQ=255)
           cgGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATa                                                                                                                             <  1:180348/151‑1 (MQ=255)
                  aaGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCa                                                                                                                      <  2:417447/151‑1 (MQ=255)
                       tGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGt                                                                                                                 >  2:576199/1‑151 (MQ=255)
                          cgcgAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTa                                                                                                               >  1:325331/1‑150 (MQ=255)
                            cgAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACg                                                                                                            >  2:174878/1‑151 (MQ=255)
                                            cATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCcgc                                                                                            >  2:15727/1‑151 (MQ=255)
                                                 gCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCa                                                                                       <  2:323455/151‑1 (MQ=255)
                                                           tcACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGcc                                                                               >  2:154169/2‑149 (MQ=255)
                                                               tGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGcac                                                                          >  1:171831/1‑150 (MQ=255)
                                                                  aCCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCt                                                                      >  1:491604/1‑151 (MQ=255)
                                                                   ccAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCt                                                                      <  2:353949/150‑1 (MQ=255)
                                                                   ccAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTg                                                                     >  2:286408/1‑151 (MQ=255)
                                                                     aaCACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGcc                                                                   >  1:72399/1‑151 (MQ=255)
                                                                       cacCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAg                                                                 <  2:167139/151‑1 (MQ=255)
                                                                          ccTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCAc                                                              >  1:409366/1‑151 (MQ=255)
                                                                                gCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCt                                                        <  1:5886/151‑1 (MQ=255)
                                                                                     aaTAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCt                                                   <  1:115267/151‑1 (MQ=255)
                                                                                      aTAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTg                                                  >  1:707995/1‑151 (MQ=255)
                                                                                        aGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCg                                                >  1:403699/1‑151 (MQ=255)
                                                                                           ttGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGgct                                             >  2:574/1‑151 (MQ=255)
                                                                                            tGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGgct                                             <  2:626382/150‑1 (MQ=255)
                                                                                               aGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGctgctg                                         <  2:658600/151‑1 (MQ=255)
                                                                                                   ggAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGgcgc                                     >  1:39013/1‑151 (MQ=255)
                                                                                                    gAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGcgcg                                    <  1:84273/151‑1 (MQ=255)
                                                                                                     aaaaTGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGcgcg                                    >  2:624489/1‑150 (MQ=255)
                                                                                                      aaaTGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTa                                  <  1:496530/151‑1 (MQ=255)
                                                                                                       aaTGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTAt                                 <  1:413003/151‑1 (MQ=255)
                                                                                                         tGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTAtgc                               >  2:562227/1‑151 (MQ=255)
                                                                                                         tGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTAtgc                               >  1:726885/1‑151 (MQ=255)
                                                                                                           tCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTAtgctg                             >  2:680589/1‑151 (MQ=255)
                                                                                                           tCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTAtgctg                             >  1:33709/1‑151 (MQ=255)
                                                                                                            cGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTAtgctgc                            >  1:653930/1‑151 (MQ=255)
                                                                                                                  aTTCGCGCCGCATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAgc                      >  2:460032/1‑151 (MQ=255)
                                                                                                                            cATTCACAGCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCg            >  1:227925/1‑151 (MQ=255)
                                                                                                                                    gCCGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGc    >  2:679853/1‑151 (MQ=255)
                                                                                                                                      cGATTCGAGCCGCCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCtc  <  1:631456/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
CTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTC  >  NC_000913/3505343‑3505627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A