Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,622,455	T→C	S1088S (AGT→AGC)	rhsB →	Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,622,455	0	T	C	100.0%	114.1 / NA	52	S1088S (AGT→AGC)	rhsB	Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/26); total (26/26)

GACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCG  >  NC_000913/3622309‑3622567

gACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCgtgg                                                                                                              >  1:614918/1‑151 (MQ=255)

       gCCGCGTCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCGCCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTccc                                                                                                       <  2:658708/151‑1 (MQ=255)

           cATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCccgccg                                                                                                   >  2:632121/1‑151 (MQ=25)

                aGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCt                                                                                              >  1:242269/1‑151 (MQ=25)

                    gATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTg                                                                                          >  1:218982/1‑151 (MQ=25)

                    gATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTg                                                                                          >  2:547961/1‑151 (MQ=25)

                    gATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTg                                                                                          >  2:627008/1‑151 (MQ=25)

                    gATTTACCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCCCCGGTGAGCGGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGCCGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGt                                                                                           <  2:256313/150‑1 (MQ=255)

                      tttACCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAACCTGCCACCGGGGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGAGGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGc                                                                                         <  1:397522/150‑1 (MQ=255)

                            aGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGc                                                                                   >  1:260584/1‑150 (MQ=25)

                            aGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCt                                                                                  >  1:202509/1‑151 (MQ=25)

                            aGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCt                                                                                  >  1:232847/1‑151 (MQ=25)

                             gCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCt                                                                                  <  1:364138/150‑1 (MQ=25)

                             gCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCt                                                                                  <  2:126795/150‑1 (MQ=25)

                             gCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTc                                                                                 >  2:129450/1‑151 (MQ=25)

                                ggggAGCTTCACGCCCCTCATCAGGGTCGAAGCTGCCACGGGTGAGCTGGCGAAAACGCGGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGGGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCg                                                                                <  2:420710/149‑1 (MQ=11)

                                ggggAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGa                                                                               >  2:54567/1‑150 (MQ=25)

                                ggggAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGCGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCCCGAc                                                                              >  2:570944/1‑151 (MQ=255)

                                ggggAGCTGCACGCCACTCACCGGGGTCGAACCTGCCACCGGGGAGCTGGCAAAACCGCAGCGCCGCAGCCGGGCGGATGCCCTTCAGCAGTCCGGGGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCt                                                                                  <  2:723640/147‑1 (MQ=11)

                                      cTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTg                                                                        >  2:159635/1‑151 (MQ=25)

                                        tCACGCCACTCATCAGGGTCGAACCTGCCGCCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCGGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTg                                                                        <  2:121002/149‑1 (MQ=255)

                                          aCGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGaa                                                                     >  2:184020/1‑150 (MQ=25)

                                            gCCACTCATCAGGGTCGAAACTGCCCCCGTTGAGCTGGCGAAAACGCAGCGCCGCAGCCGGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCCGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTgg                                                                       <  2:207633/146‑1 (MQ=255)

                                            gCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAg                                                                   >  2:334070/1‑150 (MQ=25)

                                              cACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTg                                                                 >  1:446609/1‑150 (MQ=25)

                                              cACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGa                                                                <  1:508813/151‑1 (MQ=25)

                                              cACTCAGCGGGGTCGAAACTGCCACCGGTGAGCTGGCGAAGACGCAGCGCCGCGGCCTGGCGGTTGCCTTTCAGCAGTCCGGGGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGa                                                                <  2:167500/151‑1 (MQ=11)

                                                  catcaGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATc                                                            >  2:729317/1‑151 (MQ=25)

                                                  catcaGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATc                                                            <  1:262095/151‑1 (MQ=25)

                                                              acctccccccGGTGAGCTGGCGAAAACGCAGCGCCGAAGCCTGGCGGATGCCCTTCCGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCgg                                                <  1:355894/143‑1 (MQ=11)

                                                              aaCTGCCCCCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGGGGCGAAGACGGTGGCAGCGTGGTGTCCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCgg                                                <  1:727834/151‑1 (MQ=255)

                                                                 tGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGAGAACAGGGGGGCAGCGAGGTGGTCCCGCGGGTGCTGGAGCGGATGCTCGGCAGGCTGGAGAGTGAAATGCTGGCTGGCCGGgtg                                             >  2:238773/1‑151 (MQ=11)

                                                                  gCCCCCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtg                                             <  2:597740/150‑1 (MQ=14)

                                                                  gCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgt                                              <  2:296080/149‑1 (MQ=14)

                                                                   ccACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtgag                                           <  1:728879/151‑1 (MQ=14)

                                                                   ccACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtg                                             >  2:627771/1‑149 (MQ=14)

                                                                    cACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtgag                                           >  2:441783/1‑150 (MQ=14)

                                                                     aCCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtgagt                                          >  2:732629/1‑150 (MQ=14)

                                                                          tGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGa                                     >  2:678338/1‑150 (MQ=14)

                                                                             gCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgc                                 <  1:736397/151‑1 (MQ=14)

                                                                                ggCGAAAACGCGGCGCCGCGGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGAGGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgcc                                <  2:738914/149‑1 (MQ=9)

                                                                                ggCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgccg                               <  2:159167/150‑1 (MQ=14)

                                                                                     aaaCGCAGCGCCGCGCCCGGGCGGATGCCCTTCAGCAGTCCGGCGGGGAAGAGGGTGGCGGCGGGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctg                         <  2:615513/151‑1 (MQ=11)

                                                                                      aaCGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctgg                        >  1:327010/1‑151 (MQ=14)

                                                                                         gCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctggctg                     >  1:73417/1‑151 (MQ=14)

                                                                                           aGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctggctg                     <  1:129453/149‑1 (MQ=14)

                                                                                               ccgcAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATc                >  2:391754/1‑150 (MQ=14)

                                                                                                    gCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCg           <  1:378535/150‑1 (MQ=14)

                                                                                                        gggggATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTg      <  1:465601/148‑1 (MQ=14)

                                                                                                        ggCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCGGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCt       <  2:368647/150‑1 (MQ=14)

                                                                                                        ggCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCt       <  1:632158/150‑1 (MQ=14)

                                                                                                             aTGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCg  >  2:573038/1‑150 (MQ=14)

GACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCG  >  NC_000913/3622309‑3622567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A