Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I2 R1	150	68.2	2132745	98.7%	2105019	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,622,455	T→C	S1088S (AGT→AGC)	rhsB →	Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,622,455	0	T	C	96.2%	108.5 / NA	52	S1088S (AGT→AGC)	rhsB	Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (29/21); minor base G (0/2); total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAG  >  NC_000913/3622313‑3622572

ggAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTGCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGgtgt                                                                                                                <  2:194936/150‑1 (MQ=255)

 gAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTc                                                                                                              >  2:710555/1‑151 (MQ=38)

   gCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTccc                                                                                                            <  1:127746/151‑1 (MQ=37)

           cAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGc                                                                                                    >  2:533209/1‑151 (MQ=25)

                gATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTg                                                                                               >  2:81326/1‑151 (MQ=25)

                 aTTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGc                                                                                              >  1:866404/1‑151 (MQ=25)

                  tttATCAGCGGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGc                                                                                              <  2:458026/150‑1 (MQ=25)

                    tATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAGAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGc                                                                                              >  2:273440/1‑148 (MQ=25)

                       cAGGCGGGGAGCTTCACGCCTCTCATCAGGGTCGAAACTGCCGCCGGTGAGCTGGCGAAAACGCAGCCCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTTTTCCCGCCGGTGCTGGTGCAGATg                                                                                         <  1:370629/150‑1 (MQ=11)

                       cAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGc                                                                                        >  2:544147/1‑151 (MQ=25)

                          ccGGGGAGCTCCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGGGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGCCCGGCGGGGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCt                                                                                       <  2:500567/149‑1 (MQ=255)

                               gAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCgg                                                                                <  1:992278/151‑1 (MQ=25)

                                  cTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTg                                                                             >  2:785383/1‑151 (MQ=25)

                                    tCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTgg                                                                            >  1:64235/1‑150 (MQ=25)

                                    tCACGCCACTCATCAGGGTCGAAACTGCCACCGGCGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTgg                                                                            >  2:84432/1‑150 (MQ=25)

                                     cACGCCACTCAGCAGGGTCGAACCTGCCACCGGTGGGCGGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCGGTCGGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTg                                                                             <  2:214440/148‑1 (MQ=11)

                                       cGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGaaa                                                                         <  1:200396/150‑1 (MQ=25)

                                        gCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGGGAAAACGCAGCGCCGCAGCGTGGCGGATGCTCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGGTCCCGCCGGTGCTGGTGCAGATGCGCGACCGGCTGGAAAg                                                                        >  2:100972/1‑150 (MQ=11)

                                         ccACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGt                                                                       <  2:563647/150‑1 (MQ=25)

                                            cTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGaa                                                                    >  1:996446/1‑150 (MQ=25)

                                                tcaGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAAt                                                                  <  2:1026667/148‑1 (MQ=25)

                                                tcaGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCt                                                               >  1:707905/1‑151 (MQ=25)

                                                  aGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTgg                                                             >  2:9210/1‑151 (MQ=25)

                                                    ggTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCt                                                           >  1:571112/1‑151 (MQ=25)

                                                        gAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGAcc                                                       >  2:970208/1‑151 (MQ=18)

                                                          accggCCACGGGTGAGCGGGCGAAACCGCGGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGGGGCGAAGAGGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCgg                                                     <  2:721667/147‑1 (MQ=11)

                                                              gCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtg                                                  >  1:1026735/1‑150 (MQ=14)

                                                               ccACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGGGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtg                                                  >  1:335918/1‑149 (MQ=14)

                                                                cACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtgag                                                >  2:682950/1‑150 (MQ=14)

                                                                    ggTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAgg                                           >  1:847103/1‑151 (MQ=14)

                                                                         gcgggCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGt                                                                       <  2:950570/115‑1 (MQ=17)

                                                                         gCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGt                                                                       >  1:950610/1‑118 (MQ=17)

                                                                         gCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAg                                       <  1:964093/150‑1 (MQ=14)

                                                                          cTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgcc                                     >  1:53206/1‑151 (MQ=14)

                                                                            ggCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgccg                                    <  2:380766/150‑1 (MQ=14)

                                                                            ggCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGCCCGGCGGCGAAGACGGTGGCAGCGTGGTGTCCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAg                                       <  2:682511/147‑1 (MQ=9)

                                                                              cGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGccgccg                                 >  2:291116/1‑151 (MQ=14)

                                                                               gAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCcgccgc                                >  1:889080/1‑151 (MQ=14)

                                                                                 aaccacggcgcccaggCCTGGGGGATGCCCTTCACGAGCCCGGCGCGGAAGGGGGGGGCAGGGTGGTGTCCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctg                              <  1:121615/136‑1 (MQ=11)

                                                                                                gCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCgg               >  2:972254/1‑151 (MQ=14)

                                                                                                 ccgggCGGATGCCCTTCAGCAGTCCGGCGGCGAAGCCGGTGGCAGCGGGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCgg               <  2:642718/147‑1 (MQ=9)

                                                                                                   tGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCt            >  1:575701/1‑151 (MQ=14)

                                                                                                    gggggATGCCCTTCAGCATGCCGGCGGCGAGGAGGGTGGCGGGGTGGTGTCCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCt            <  2:236242/147‑1 (MQ=11)

                                                                                                    ggCGGATGCCCTTCAGCAGCCCGGCGGCGAAGACGGGGGCAGCGGGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCt            <  2:282495/150‑1 (MQ=9)

                                                                                                     gCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTg           >  1:1012013/1‑150 (MQ=14)

                                                                                                      cGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGAc         >  2:641312/1‑151 (MQ=14)

                                                                                                       ggATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGAcc        <  1:81327/151‑1 (MQ=14)

                                                                                                         aTGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGt      <  1:310525/151‑1 (MQ=14)

                                                                                                            cccTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTgg    >  1:534183/1‑150 (MQ=14)

                                                                                                            cccTACAGCAGCCCGGGGGAGAAGACGTTGGAAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGa   <  2:722696/151‑1 (MQ=11)

                                                                                                             ccTTCAGCGGTCGGGGGGCGAAGAGGGTGGCGGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAg  <  2:7761/151‑1 (MQ=11)

                                                                                                             ccTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTgg    >  1:176696/1‑149 (MQ=11)

GGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAG  >  NC_000913/3622313‑3622572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A