Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I1 R1 163 55.0 1534755 98.6% 1513268 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,811,334 A→G R28R (CGT→CGC)  rpmG ← 50S ribosomal subunit protein L33

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,811,3340AG100.0% 171.0 / NA 47R28R (CGT→CGC) rpmG50S ribosomal subunit protein L33
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/24);  total (23/24)

GGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGCCACGGGCACGCAGTTCAGCCAGAA  >  NC_000913/3811190‑3811475
                                                                                                                                                |                                                                                                                                             
ggCAAGCAGATAACAAAAAGCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGtt                                                                                                                                           <  1:110848/149‑1 (MQ=255)
    aGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGttct                                                                                                                                     <  2:754476/151‑1 (MQ=255)
         aTAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGt                                                                                                                                 >  1:268335/1‑150 (MQ=255)
               aaaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGt                                                                                                                           >  2:188080/1‑150 (MQ=255)
                    ccccGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGa                                                                                                                      >  2:46955/1‑150 (MQ=255)
                     cccGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGa                                                                                                                      >  2:255403/1‑149 (MQ=255)
                              gCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGt                                                                                                            >  2:67104/1‑150 (MQ=255)
                                gAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTCTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTAcc                                                                                                         >  2:309817/1‑151 (MQ=255)
                                             aCATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAAc                                                                                             <  2:634544/150‑1 (MQ=255)
                                              cATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAAcct                                                                                           <  2:474708/151‑2 (MQ=255)
                                              cATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAAcc                                                                                            >  1:678633/1‑150 (MQ=255)
                                               aTCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAg                                                                                          <  2:442989/151‑1 (MQ=255)
                                                 cAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCt                                                                                        >  2:707285/1‑151 (MQ=255)
                                                 cAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCt                                                                                        >  2:185720/1‑151 (MQ=255)
                                                  aaaGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCtt                                                                                       >  1:765986/1‑151 (MQ=255)
                                                    aGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGa                                                                                     <  2:289509/151‑1 (MQ=255)
                                                    aGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGa                                                                                     <  2:140842/151‑1 (MQ=255)
                                                    aGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGa                                                                                     <  2:170083/151‑1 (MQ=255)
                                                          aaTTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTtct                                                                               <  2:408490/151‑1 (MQ=255)
                                                             tattTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCAc                                                                            >  2:2718/1‑151 (MQ=255)
                                                              attTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCAc                                                                            <  1:687186/150‑1 (MQ=255)
                                                                ttGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGa                                                                          <  2:110127/150‑1 (MQ=255)
                                                                   aTTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATAc                                                                      >  2:12656/1‑151 (MQ=255)
                                                                   aTTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATAc                                                                      <  2:646063/151‑1 (MQ=255)
                                                                   aTTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATAc                                                                      >  2:161678/1‑151 (MQ=255)
                                                                     tttCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCt                                                                    >  2:342901/1‑151 (MQ=255)
                                                                      ttCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCtt                                                                   >  1:726636/1‑151 (MQ=255)
                                                                      ttCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCt                                                                    <  1:669193/150‑1 (MQ=255)
                                                                        cGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCttt                                                                  <  2:765939/150‑1 (MQ=255)
                                                                        cGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTa                                                                 <  2:30815/151‑1 (MQ=255)
                                                                         gCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCttt                                                                  >  1:592604/1‑149 (MQ=255)
                                                                            tcttTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGcc                                                              <  1:337950/150‑1 (MQ=255)
                                                                                       cACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTc                                                  <  1:46956/151‑1 (MQ=255)
                                                                                          gTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCctc                                               >  1:331921/1‑151 (MQ=255)
                                                                                            gCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCctct                                              <  2:476651/150‑1 (MQ=255)
                                                                                            gCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTa                                             <  2:669213/151‑1 (MQ=255)
                                                                                               ggCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAagt                                          >  2:25386/1‑151 (MQ=255)
                                                                                                gCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAagta                                         <  1:547772/151‑1 (MQ=255)
                                                                                                        cTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAagtacttagtac                                 >  1:422326/1‑151 (MQ=255)
                                                                                                               gAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGc                          <  2:419354/151‑1 (MQ=255)
                                                                                                                aaTTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGc                          >  2:509713/1‑150 (MQ=255)
                                                                                                                       tCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGCCACGGGc                   <  1:262025/150‑1 (MQ=255)
                                                                                                                            tCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACGTTTCGCCACGGGCACGCa              <  2:508252/150‑1 (MQ=255)
                                                                                                                              cAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGCCACGGGCACGCAGtt           >  1:661380/1‑151 (MQ=255)
                                                                                                                                  ttttCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGCCACGGGCACGCAGTTCAGc       >  1:89879/1‑151 (MQ=255)
                                                                                                                                       cGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGCCACGGGCACGCAGTTCAGCCAGa   >  1:359060/1‑150 (MQ=255)
                                                                                                                                        gtcTTAGTGCGTTTGTTCTTCGTTGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGCCACGGGCACGCAGTTCAGCCAGaa  <  2:62900/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                             
GGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGCCACGGGCACGCAGTTCAGCCAGAA  >  NC_000913/3811190‑3811475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A