Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I1 R1 163 55.0 1534755 98.6% 1513268 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,816,407 G→A pseudogene (143/669 nt) rph ← ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,816,4070GA100.0% 220.3 / NA 55pseudogene (143/669 nt)rphribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/27);  total (28/27)

CACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCGGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATT  >  NC_000913/3816261‑3816552
                                                                                                                                                  |                                                                                                                                                 
cACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCAc                                                                                                                                               >  1:382309/1‑151 (MQ=255)
 aCGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACg                                                                                                                                              >  1:81240/1‑151 (MQ=255)
 aCGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACg                                                                                                                                              <  2:35742/151‑1 (MQ=255)
        aTCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTTCCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCcttctt                                                                                                                                       <  2:447681/151‑1 (MQ=255)
         tCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCttcttc                                                                                                                                      >  2:598606/1‑151 (MQ=255)
           cgaagCTGGATTTCCATGGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGAAGAACGTGGCAGCATGCCGTATTCTGCGGTGATCCAGCTCTGGCACTGACCTTTCAGGAAGCGCAGGACGCcttctt                                                                                                                                       >  2:527768/5‑148 (MQ=255)
           aGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCa                                                                                                                                     >  2:208176/1‑150 (MQ=255)
                       tCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTa                                                                                                                         >  2:236461/1‑150 (MQ=255)
                        ccATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTac                                                                                                                        >  2:117779/1‑150 (MQ=255)
                         cATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTacac                                                                                                                      <  1:761333/151‑1 (MQ=255)
                           ttGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTAcaca                                                                                                                     >  2:356593/1‑150 (MQ=255)
                            tgtgCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACacaa                                                                                                                    <  1:467834/150‑1 (MQ=255)
                            tgtgCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACacaa                                                                                                                    >  1:268746/1‑150 (MQ=255)
                            tgtgCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACacaa                                                                                                                    >  1:365443/1‑150 (MQ=255)
                                 gTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACttt                                                                                                              <  1:735847/151‑1 (MQ=255)
                                   ccACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTgg                                                                                                            <  1:516124/151‑1 (MQ=255)
                                    cACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGt                                                                                                           <  1:489399/151‑1 (MQ=255)
                                       ccTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATc                                                                                                        <  1:215526/151‑1 (MQ=255)
                                          gCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGcc                                                                                                     >  1:270702/1‑151 (MQ=255)
                                          gCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGcc                                                                                                     >  2:578264/1‑151 (MQ=255)
                                             tACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCaa                                                                                                   >  2:574603/1‑150 (MQ=255)
                                               ccTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAAtt                                                                                                <  1:116609/151‑1 (MQ=255)
                                               ccTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAAt                                                                                                 <  1:324074/150‑1 (MQ=255)
                                                cTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTc                                                                                               >  1:372994/1‑151 (MQ=255)
                                                  ttCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACTCTTTGGTATCGCCAAATTCg                                                                                              <  1:576383/150‑1 (MQ=255)
                                                   tCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGa                                                                                             <  2:710786/150‑1 (MQ=255)
                                                     gccgcTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCATGAAGCGCAGCACGCCTTCTTCAATAGATGCGGTACACAACACTTTGGTATCGCCAAATTCGGCCa                                                                                          <  1:29914/151‑1 (MQ=255)
                                                      ccgcTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGAcc                                                                                           >  1:238643/1‑149 (MQ=255)
                                                         cTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCCGccc                                                                                      >  2:741949/1‑149 (MQ=255)
                                                           tCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCAc                                                                                      <  1:557032/149‑1 (MQ=255)
                                                                aGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCt                                                                               >  1:638591/1‑151 (MQ=255)
                                                                  cGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTc                                                                             <  1:363938/151‑1 (MQ=255)
                                                                     tACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTg                                                                           <  1:327393/150‑1 (MQ=255)
                                                                     tACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGc                                                                          >  2:477974/1‑151 (MQ=255)
                                                                           tgtgGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGttt                                                                    >  1:439402/1‑151 (MQ=255)
                                                                            gtgGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGtttt                                                                   <  2:344814/151‑1 (MQ=255)
                                                                                        tGGCAGCATGCCGTGCTCTGCGGTGATCCAGCCCTGGCCCTGACCTTGCAGGAAGCGCATCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAAGTCGACCAGCACCGAGCCTTCTGCAGGTTTTGTATAGTTACg                                                        <  1:432251/150‑1 (MQ=255)
                                                                                         ggCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACg                                                        >  1:28867/1‑149 (MQ=255)
                                                                                              caTGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAgg                                                 >  1:5355/1‑151 (MQ=255)
                                                                                                       ctctGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACgg                                          <  1:125482/149‑1 (MQ=255)
                                                                                                        tctGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGAc                                       >  1:320427/1‑151 (MQ=255)
                                                                                                         ctGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGAc                                       >  1:213081/1‑150 (MQ=255)
                                                                                                                gATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGa                               <  1:222813/151‑1 (MQ=255)
                                                                                                                gATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGa                               <  2:646934/151‑1 (MQ=255)
                                                                                                                   ccAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGatt                             <  2:283337/150‑1 (MQ=255)
                                                                                                                    cAGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGattat                           >  1:36261/1‑151 (MQ=255)
                                                                                                                     aGCCCTGGCCCTGACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGattatt                          >  1:18359/1‑151 (MQ=255)
                                                                                                                           ggccctgACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACg                    <  1:672375/151‑1 (MQ=255)
                                                                                                                                 gcccTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGGATTGCCCAATTCGACCAGCACCGAACCTGCTGCATGTTTTGTATAGTTGCGATGCAGGGGAACGGGACGCACCTGCTTATAGGGACGGCctg               >  2:316399/3‑150 (MQ=255)
                                                                                                                                 gACCTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCctgc              <  1:389546/151‑1 (MQ=255)
                                                                                                                                    cTTTCAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTgg           >  2:607957/1‑151 (MQ=255)
                                                                                                                                        cAGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACg        <  2:188380/150‑1 (MQ=255)
                                                                                                                                         aGGAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCa      <  2:490947/151‑1 (MQ=255)
                                                                                                                                          ggAAGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCat     >  1:292920/1‑151 (MQ=255)
                                                                                                                                             aGCGCAGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATAtt  <  2:16025/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCGGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATT  >  NC_000913/3816261‑3816552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A