Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,223,586	C→T	V15I (GTT→ATT)	iclR ←	transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,223,586	0	C	T	100.0%	204.8 / NA	51	V15I (GTT→ATT)	iclR	transcriptional repressor
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (10/41); total (10/41)

AGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTT  >  NC_000913/4223438‑4223720

aGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAAtgg                                                                                                                                      <  1:23810/151‑1 (MQ=255)

               gTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGcc                                                                                                                         >  2:291423/1‑149 (MQ=255)

                       gggTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTcgcg                                                                                                               <  1:471747/151‑1 (MQ=255)

                        ggTATCCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTGCTGCCGCGTTTCGCgg                                                                                                              <  2:492187/151‑1 (MQ=255)

                         gTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCggg                                                                                                             <  2:96788/151‑1 (MQ=255)

                             cccGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCTATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAAt                                                                                                          <  2:75141/150‑1 (MQ=255)

                              ccGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATgg                                                                                                        <  1:139295/151‑1 (MQ=255)

                              ccGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATgg                                                                                                        <  2:649589/151‑1 (MQ=255)

                               cGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATggg                                                                                                       <  1:626193/151‑1 (MQ=255)

                                     gttgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGAc                                                                                                 <  1:559230/151‑1 (MQ=255)

                                        gcgcCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCAt                                                                                              <  2:592348/151‑1 (MQ=255)

                                         cgcCAGTTCCGTGAGTGCCACCCTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCTTTTCGCGGGAATGGGTGCGACCAt                                                                                              <  2:113203/150‑1 (MQ=255)

                                           ccAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGAc                                                                                           <  1:638724/151‑1 (MQ=255)

                                                 ccGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGtct                                                                                      <  1:67526/150‑1 (MQ=255)

                                                       gTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCtttttt                                                                               <  1:172591/151‑1 (MQ=255)

                                                        tGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGGTTTCTGCCGCGGTTCGCGGGGATGGGTGCGACCATGACAGTCTCtttttt                                                                               >  2:394647/1‑150 (MQ=255)

                                                         gCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCt                                                                             <  1:511700/151‑1 (MQ=255)

                                                          ccACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTg                                                                            <  2:175938/151‑1 (MQ=255)

                                                           cacaCTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGt                                                                           <  1:641754/151‑1 (MQ=255)

                                                           cacaCTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGt                                                                           <  2:710920/151‑1 (MQ=255)

                                                              acTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATc                                                                        <  1:86092/151‑1 (MQ=255)

                                                               cTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATc                                                                        <  2:190054/150‑1 (MQ=255)

                                                                 gCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCg                                                                       <  1:665959/149‑1 (MQ=255)

                                                                 gCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCg                                                                       <  2:761540/149‑1 (MQ=255)

                                                                 gCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTg                                                                     <  1:184159/151‑1 (MQ=255)

                                                                  ccATTGGATTCGGCAATCCACTCCAGTAATTTCAGGACACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGTGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCg                                                                       <  2:133920/148‑1 (MQ=255)

                                                                   cATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGa                                                                   <  1:665008/151‑1 (MQ=255)

                                                                       ggATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAt                                                                <  1:285303/150‑1 (MQ=255)

                                                                       ggATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAt                                                                <  1:733944/150‑1 (MQ=255)

                                                                       ggATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGGTTTTTGCCCCGTTTCGCGGGAAAGGGGGGGACCATGACAGTCTCTTTTTTTTGTATCGTGGgaa                                                                 >  2:546344/1‑149 (MQ=255)

                                                                        gATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAtca                                                              <  2:655429/151‑1 (MQ=255)

                                                                        gATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAtca                                                              <  2:560614/151‑1 (MQ=255)

                                                                         aTTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTTTGTATCGTGGAAAtcat                                                             >  2:429643/1‑151 (MQ=255)

                                                                         aTTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAtcat                                                             <  1:671541/151‑1 (MQ=255)

                                                                            cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAtcatttt                                                          <  1:645306/151‑1 (MQ=255)

                                                                                   ccACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCAttttt                                                   <  2:352704/151‑1 (MQ=255)

                                                                                        ccAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGt                                              <  1:337341/151‑1 (MQ=255)

                                                                                          aGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATtttttttttt                                             >  1:466572/1‑150 (MQ=255)

                                                                                           gTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAg                                           <  2:615331/151‑1 (MQ=255)

                                                                                             aaTTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGc                                          <  2:558186/150‑1 (MQ=255)

                                                                                                ttCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGGTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGGAATCATTTTCATTTTTATTGTTAGCTaa                                       >  2:16173/1‑150 (MQ=255)

                                                                                                    ggCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCaa                                  <  2:236741/151‑1 (MQ=255)

                                                                                                    ggCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCaa                                  <  2:372428/151‑1 (MQ=255)

                                                                                                               aaaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaa                       <  1:240214/151‑1 (MQ=255)

                                                                                                               aaaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga                        <  2:161066/150‑1 (MQ=255)

                                                                                                                aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTTTGCCGCGTTTCGCGGGAATGGGGGCGACCCTGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTTTTTTTAGCTAATGCAATAGTTactgaa                       >  1:724688/1‑150 (MQ=255)

                                                                                                                   aCTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaactga                   <  2:721830/151‑1 (MQ=255)

                                                                                                                    cTGAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGGTTTTTGCCGCGTTTCGCGGGAATGGGGGCGGCCATGACAGGCTCTTTTTTTTGTATTGGGGGAATCATTTTTATTTTTATTTTTAGCTAATGCAATAGTTtttgaa                       >  2:234387/1‑146 (MQ=255)

                                                                                                                      gAACCTGTCCAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATcc                <  2:632376/151‑1 (MQ=255)

                                                                                                                              ccAGTCGCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGtt        <  1:349079/151‑1 (MQ=255)

                                                                                                                                    gCTGGTGCGGTGGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGGGGGACCATGAGAATCTCTTTTTTTTGTATCGTGGAAATCCTTTTTATTTTTTTTGTTAAATAATGCAATAGTTACTGAACTGATCCGGTGAGTTAATGt   >  1:91990/1‑150 (MQ=255)

                                                                                                                                      tGGTGCGGTGGCAATGGCGGGGTTTCTGCCGCGTTTCGCGGGGATGGGTGCGGCCATGACAGGCTCTTTTTTCTGTATCGTGGGAATCATTTTCATTTTTTTTGTTAGCTAATGCAATAATTACTGAAATGATCCGATTAGTTAATGtt  >  2:209877/1‑149 (MQ=255)

AGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTT  >  NC_000913/4223438‑4223720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A