Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,270,398	A→G	N54S (AAC→AGC)	yjbQ →	thiamine phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,270,398	0	A	G	100.0%	174.7 / NA	47	N54S (AAC→AGC)	yjbQ	thiamine phosphate synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/16); total (31/16)

CGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTAT  >  NC_000913/4270254‑4270541

cGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCg                                                                                                                                            >  1:91166/1‑150 (MQ=255)

cGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGa                                                                                                                                           <  1:168781/151‑1 (MQ=255)

 gCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCCTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGAt                                                                                                                                          >  1:68504/1‑151 (MQ=255)

      cGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCAc                                                                                                                                     <  2:6753/151‑1 (MQ=255)

        cTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCg                                                                                                                                   >  2:553910/1‑151 (MQ=255)

              gcCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGc                                                                                                                              >  2:681615/1‑150 (MQ=255)

                   aTCTCGTGGGGTTCATCTGGTAACGGCTGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTCACATTGGCTTACTGCATCTGCTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACCCCACGAc                                                                                                                        >  2:44129/1‑151 (MQ=255)

                           ggTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCg                                                                                                                <  1:452894/151‑1 (MQ=255)

                                cATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGttttt                                                                                                           <  2:718570/151‑1 (MQ=255)

                                  tCTGGTAACGGGTGAAATTCTGAATCAGCGGGCGGAGATGCCGCGCGGTAACATCGGCGTACTGCTGCTGTTGCTGCAACATACCTCCGCCGCGCTGACACTTAATGAGAGGTGCGATCCCACCGTGCGCCACGACATGGAGCGtgtttt                                                                                                          >  2:291601/1‑150 (MQ=255)

                                            gATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCAcc                                                                                                >  1:513766/1‑150 (MQ=255)

                                             aTGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGt                                                                                              >  2:344727/1‑151 (MQ=255)

                                               gAAATTCTGAATCAGGTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCATCATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGtt                                                                                             <  1:465319/150‑1 (MQ=255)

                                                         aTCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACgg                                                                                  >  2:732513/1‑151 (MQ=255)

                                                           cAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGa                                                                                 <  1:121781/150‑1 (MQ=255)

                                                           cAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCACCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGa                                                                                 >  1:164845/1‑150 (MQ=255)

                                                            aGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGaa                                                                                >  1:155991/1‑150 (MQ=255)

                                                               tggctgATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAAtt                                                                             >  2:364277/1‑150 (MQ=255)

                                                               tggctgATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAAtt                                                                             >  1:675502/1‑150 (MQ=255)

                                                                ggctgATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTAt                                                                           >  1:602715/1‑151 (MQ=255)

                                                                ggctgATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTAt                                                                           >  1:381644/1‑151 (MQ=255)

                                                                    gATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAg                                                                        <  2:615068/150‑1 (MQ=255)

                                                                    gATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGc                                                                       >  2:700249/1‑151 (MQ=255)

                                                                     atatGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGc                                                                       >  1:432614/1‑150 (MQ=255)

                                                                       atGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCAt                                                                     <  2:249093/150‑1 (MQ=255)

                                                                       atGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATg                                                                    >  1:512179/1‑151 (MQ=255)

                                                                         gCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGAc                                                                  <  1:5993/151‑1 (MQ=255)

                                                                          ccGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACt                                                                 <  2:256189/151‑1 (MQ=255)

                                                                          ccGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACt                                                                 >  2:310388/1‑151 (MQ=255)

                                                                            gcgcgTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTAt                                                               >  1:588037/1‑151 (MQ=255)

                                                                              gcgTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATg                                                              >  1:502825/1‑150 (MQ=255)

                                                                                         ggCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACg                                                  <  1:740812/151‑1 (MQ=255)

                                                                                                tGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGcc                                           >  2:66558/1‑151 (MQ=255)

                                                                                                  cATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCtt                                         >  1:20965/1‑151 (MQ=255)

                                                                                                       gTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCat                                    >  2:709029/1‑151 (MQ=255)

                                                                                                       gTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCat                                    >  2:605584/1‑151 (MQ=255)

                                                                                                           cttcAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATc                                 <  2:427038/147‑1 (MQ=255)

                                                                                                              cAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCaa                               <  1:153071/149‑1 (MQ=255)

                                                                                                              cAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAAt                             <  1:690654/151‑1 (MQ=255)

                                                                                                                    aCCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCa                        <  2:393981/150‑1 (MQ=255)

                                                                                                                       tCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGc                    >  1:299834/1‑151 (MQ=255)

                                                                                                                         cGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTg                  >  1:648442/1‑151 (MQ=255)

                                                                                                                         cGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTg                  >  1:315058/1‑151 (MQ=255)

                                                                                                                                  gACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATc          <  2:685842/150‑1 (MQ=255)

                                                                                                                                     acTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCtt      >  1:407309/1‑151 (MQ=255)

                                                                                                                                      cTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTg     >  1:596715/1‑151 (MQ=255)

                                                                                                                                         aaTGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTAt  >  2:204613/1‑151 (MQ=255)

CGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTAT  >  NC_000913/4270254‑4270541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A