Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I1 R1 163 55.0 1534755 98.6% 1513268 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,503,797 A→G intergenic (‑366/‑261) insG ← / → yjhB IS4 transposase/putative MFS transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,503,7970AG100.0% 50.7 / NA 15intergenic (‑366/‑261)insG/yjhBIS4 transposase/putative MFS transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/12);  total (3/12)

AAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAATGAATAAGTCAATTTTTGAAGCAGGGTTGAACTGTGCCAGTGGTGGAATATCTGGCGAACATTGTTTTTTGTTGGTGACCCAAAACTGTAGGTGGGTATCAGTTTTATCTTTCATAGAGTGAAATATGTTAAGAAGAAATGGAGGAAAAGATTGTA  >  NC_000913/4503657‑4503930
                                                                                                                                            |                                                                                                                                     
aaGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAATGAATAAGTCAATTTTTGAAGCGGGGTTGAACt                                                                                                                             <  1:430780/151‑1 (MQ=255)
 aGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAATGAATAAGTCAATTTTTGAAGCGGGGTTGAACtg                                                                                                                            >  2:92583/1‑151 (MQ=255)
  gAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAATGAATAAGTCAATTTTTGAAGCGGGGTTGAACtgt                                                                                                                           <  2:672814/151‑1 (MQ=255)
          gCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAATGAATAAGTCAATTTTTGAAGCGGGGTTGAACTGTGCCAgtg                                                                                                                    <  2:339982/150‑1 (MQ=255)
                            aaCGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAATGAATAAGTCAATTTTTGAAGCGGGGTTGAACTGTGCCAGTGGTGGAATATCTGGCGAACa                                                                                                 <  2:361551/151‑1 (MQ=255)
                                   gCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAATGAATAAGTCAATTTTTGAAGCGGGGTTGAACTGTGCCAGTGGTGGAATATCTGGCGAACATTGtttt                                                                                          <  1:37853/151‑1 (MQ=255)
                                      aaaGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAATGAATAAGTCAATTTTTGAAGCGGGGTTGAACTGTGCCAGTGGTGGAATATCTGGCGAACATTGttttt                                                                                         <  1:753420/149‑1 (MQ=255)
                                                         cTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAATGAATAAGTCAATTTTTGAAGCGGGGTTGAACTGTGCCAGTGGTGGAATATCTGGCGAACATTGTTTTTTGTTGGTGACCCAAAACTGt                                                                     >  1:574204/1‑150 (MQ=255)
                                                                           gggAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAATGAATAAGTCAATTTTTGAAGCGGGGTTGAACTGTGCCAGTGGTGGAATATCTGGCGAACATTGTTTTTTGTTGGTGACCCAAAACTGTAGGTGGGTATCAGTTTTAt                                                  <  1:8997/151‑1 (MQ=255)
                                                                              aGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAATGAATAAGTCAATTTTTGAAGCGGGGTTGAACTGTGCCAGTGGTGGAATATCTGGCGAACATTGTTTTTTGTTGGTGACCCAAAACTGTAGGTGGGTATCAGTTTTATCtt                                               <  1:92584/151‑1 (MQ=255)
                                                                                  ttATTCAACCTGAACGGACTACGAAAATGAGCACAATGAATAAGTCAATTTTTGAAGCGGGGTTGAACTGTGCCAGTGGTGGAATATCTGGCGAACATTGTTTTTTGTTGGTGACCCAAAACTGTAGGTGGGTATCAGTTTTATCTTTCa                                            <  2:72652/150‑1 (MQ=255)
                                                                                      tCAACCTGAACGGACTACGAAAATGAGCACAATGAATAAGTCAATTTTTGAAGCGGGGTTGAACTGTGCCAGTGGTGGAATATCTGGCGAACATTGTTTTTTGTTGGTGACCCAAAACTGTAGGTGGGTATCAGTTTTATCTTTCATaga                                        <  1:503232/150‑1 (MQ=255)
                                                                                                 ggACTACGAAAATGAGCACAATGAATAAGTCAATTTTTGAAGCGGGGTTGAACTGTGCCAGTGGTGGAATATCTGGCGAACATTGTTTTTTGTTGGTGACCCAAAACTGTAGGTGGGTATCAGTTTTATCTTTCATAGAGTGAAATATGtt                            >  2:342139/1‑151 (MQ=255)
                                                                                                                 cacaATGAATAAGTCAATTTTTGAAGCGGGGTTGAACTGTGCCAGTGGTGGAATATCTGGCGAACATTGTTTTTTGTTGGTGACCCAAAACTGTAGGTGGGTATCAGTTTTATCTTTCATAGAGTGAAATATGTTAAGAAGAAATggagga            <  2:420454/151‑1 (MQ=255)
                                                                                                                           aaGTCAATTTTTGAAGCGGGGTTGAACTGTGCCAGTGGTGGAATATCTGGCGAACATTGTTTTTTGTTGGTGACCCAAAACTGTAGGTGGGTATCAGTTTTATCTTTCATAGAGTGAAATATGTTAAGAAGAAATGGAGGAAAAGATTGTa  <  2:547318/151‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                     
AAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAATGAATAAGTCAATTTTTGAAGCAGGGTTGAACTGTGCCAGTGGTGGAATATCTGGCGAACATTGTTTTTTGTTGGTGACCCAAAACTGTAGGTGGGTATCAGTTTTATCTTTCATAGAGTGAAATATGTTAAGAAGAAATGGAGGAAAAGATTGTA  >  NC_000913/4503657‑4503930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A