Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I1 R1 157 93.7 2947622 98.1% 2891617 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,432,487 A→G Y358Y (TAT→TAC)  smf ← DNA recombination‑mediator A family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,432,4870AG98.1% 383.6 / ‑1.3 105Y358Y (TAT→TAC) smfDNA recombination‑mediator A family protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (46/57);  total (47/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

Only 100 of 105 total aligned reads displayed.

TGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACATAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGTTAGCCAGGAGCTCAGGAAAT  >  NC_000913/3432341‑3432634
                                                                                                                                                  |                                                                                                                                                   
tGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAgc                                                                                                                                                 >  2:541161/1‑151 (MQ=255)
tGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGACCTCCTCAATCGGACGTAgc                                                                                                                                                 >  2:1192110/1‑151 (MQ=255)
 gTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAgcc                                                                                                                                                <  1:854255/151‑1 (MQ=255)
    gATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAgccgcc                                                                                                                                             >  2:224558/1‑151 (MQ=255)
       atCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGccgccg                                                                                                                                            >  1:1127731/1‑149 (MQ=255)
       atCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCgg                                                                                                                                           <  2:1389025/150‑1 (MQ=255)
           tctcGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTa                                                                                                                                        <  1:216303/149‑1 (MQ=255)
           tctcGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTa                                                                                                                                        <  1:1237622/149‑1 (MQ=255)
            ctcGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTAc                                                                                                                                       >  2:1374647/1‑149 (MQ=255)
              cGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACa                                                                                                                                      <  1:405820/148‑1 (MQ=255)
              cGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCt                                                                                                                                   <  2:510620/151‑1 (MQ=255)
               gCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTg                                                                                                                                  <  1:449259/151‑1 (MQ=255)
               gCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTg                                                                                                                                  <  1:81993/151‑1 (MQ=255)
               gCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACATAGCCGCCGGGTACAGCTg                                                                                                                                  >  1:318675/1‑151 (MQ=255)
                 tCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCg                                                                                                                                >  2:496999/1‑151 (MQ=255)
                 tCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCg                                                                                                                                >  2:290502/1‑151 (MQ=255)
                  cAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCg                                                                                                                                <  1:1048155/150‑1 (MQ=255)
                  cAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCg                                                                                                                                >  2:1040491/1‑150 (MQ=255)
                   aaaaCCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGat                                                                                                                              >  1:41809/1‑151 (MQ=255)
                   aaaaCCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGat                                                                                                                              >  1:1070987/1‑151 (MQ=255)
                    aaaCCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGatc                                                                                                                             >  1:420292/1‑151 (MQ=255)
                      aCCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGatcc                                                                                                                            >  1:471285/1‑150 (MQ=255)
                       ccTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGatccat                                                                                                                          >  1:459468/1‑151 (MQ=255)
                        cTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGatccatc                                                                                                                         >  1:1166830/1‑151 (MQ=255)
                           cGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTg                                                                                                                      <  1:297706/151‑1 (MQ=255)
                           cGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTg                                                                                                                      >  2:1435155/1‑151 (MQ=255)
                             tCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCt                                                                                                                    >  1:1262526/1‑151 (MQ=255)
                                  aaGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACt                                                                                                                <  2:144539/150‑1 (MQ=255)
                                  aaGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTc                                                                                                               <  1:1018874/151‑1 (MQ=255)
                                  aaGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTc                                                                                                               >  2:4603/1‑151 (MQ=255)
                                   aGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTcc                                                                                                              <  1:1227013/151‑1 (MQ=255)
                                    gATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCa                                                                                                             <  1:1089124/151‑1 (MQ=255)
                                          gTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTc                                                                                                         <  1:4603/149‑1 (MQ=255)
                                          gTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTc                                                                                                         <  2:930987/149‑1 (MQ=255)
                                           ttaaaGTTTGTC‑TGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGag                                                                                                      <  2:793510/147‑1 (MQ=255)
                                           ttCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGGTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTc                                                                                                         >  2:184604/1‑148 (MQ=255)
                                              aaGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGagt                                                                                                     >  1:563352/1‑149 (MQ=255)
                                               aGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGagtagt                                                                                                  >  1:429137/1‑151 (MQ=255)
                                                    gTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGt                                                                                             <  1:1309313/151‑1 (MQ=255)
                                                      cTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTa                                                                                            >  2:91382/1‑150 (MQ=255)
                                                       ttGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAAc                                                                                          >  1:1099764/1‑151 (MQ=255)
                                                          aTCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTAc                                                                                       <  2:866001/151‑1 (MQ=255)
                                                          aTCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTAc                                                                                       <  2:620433/151‑1 (MQ=255)
                                                             cacaCGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACctc                                                                                    >  2:395328/1‑151 (MQ=255)
                                                              acacGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACctct                                                                                   >  1:894607/1‑151 (MQ=255)
                                                              acacGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACctct                                                                                   >  1:618409/1‑151 (MQ=255)
                                                              acacGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACATAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACctct                                                                                   <  2:318637/151‑1 (MQ=255)
                                                               cacGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTg                                                                                  <  2:1070886/151‑1 (MQ=255)
                                                                  gCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGca                                                                               <  1:1160580/151‑1 (MQ=255)
                                                                        cAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAgg                                                                           <  1:351565/149‑1 (MQ=255)
                                                                          gCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTgg                                                                       >  2:1053285/1‑151 (MQ=255)
                                                                               tgtgtgAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGc                                                                  >  1:316321/1‑151 (MQ=255)
                                                                                gtgtgAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCa                                                                 <  1:535733/151‑1 (MQ=255)
                                                                                  gtgAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCAc                                                                <  1:1225798/150‑1 (MQ=255)
                                                                                  gtgAATATAGGTTACAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACg                                                               >  2:615446/1‑151 (MQ=255)
                                                                                    gAATATAGTTTTCAAACAAATACATTAGGCCGTCGAACATGGCAGGCCCTCCTCAAACGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGTCACGtt                                                             <  2:1020524/151‑1 (MQ=255)
                                                                                    gAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGtt                                                             <  2:516117/151‑1 (MQ=255)
                                                                                    gAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGtt                                                             >  1:152123/1‑151 (MQ=255)
                                                                                        ataGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAg                                                          <  2:1313415/150‑1 (MQ=255)
                                                                                         taGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGcg                                                        >  2:1332919/1‑151 (MQ=255)
                                                                                           ggTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGcgac                                                      <  2:543915/151‑1 (MQ=255)
                                                                                           ggTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGcgac                                                      >  2:263785/1‑151 (MQ=255)
                                                                                           ggTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGcg                                                        <  1:816849/149‑1 (MQ=255)
                                                                                             tttCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGcgacg                                                     >  2:861014/1‑150 (MQ=255)
                                                                                               tCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCgacgac                                                   <  2:420236/150‑1 (MQ=255)
                                                                                                caaacaaaTACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGacgacg                                                  <  1:988097/150‑1 (MQ=255)
                                                                                                  aacaaaTACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCa                                               <  2:1209399/151‑1 (MQ=255)
                                                                                                  aacaaaTACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCa                                               <  2:13369/151‑1 (MQ=255)
                                                                                                   acaaaTACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCa                                               >  2:412423/1‑150 (MQ=255)
                                                                                                     aaaTACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACa                                            <  2:176879/151‑1 (MQ=255)
                                                                                                         aCATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGgtg                                        >  2:417446/1‑151 (MQ=255)
                                                                                                          cATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGgtgt                                       >  2:592887/1‑151 (MQ=255)
                                                                                                           aTTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGtt                                      <  2:922773/151‑1 (MQ=255)
                                                                                                             tAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTa                                     <  1:1258190/150‑1 (MQ=255)
                                                                                                              aGTACGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTa                                     >  1:1386324/1‑149 (MQ=255)
                                                                                                                  cGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCAt                               <  1:961132/151‑1 (MQ=255)
                                                                                                                  cGTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCAt                               <  1:1090373/151‑1 (MQ=255)
                                                                                                                   gTCGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCAtc                              <  1:290534/151‑1 (MQ=255)
                                                                                                                     cGAACATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCAtctc                            >  1:111452/1‑151 (MQ=255)
                                                                                                                       aaCATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCt                          <  1:633883/151‑1 (MQ=255)
                                                                                                                        aCATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCt                          >  1:988380/1‑150 (MQ=255)
                                                                                                                        aCATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCt                          >  2:461237/1‑150 (MQ=255)
                                                                                                                         cATGGCATGCCCTCCTCAATCGGACGTCGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTa                         <  1:1170970/150‑1 (MQ=255)
                                                                                                                         cATGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTAc                        <  2:1448284/151‑1 (MQ=255)
                                                                                                                          aTGGCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACg                       >  1:1074116/1‑151 (MQ=255)
                                                                                                                            ggCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGtt                     <  2:459413/151‑1 (MQ=255)
                                                                                                                            ggCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGtt                     <  2:627418/151‑1 (MQ=255)
                                                                                                                            ggCATGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGt                      <  2:158690/150‑1 (MQ=255)
                                                                                                                               aTGCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGTTAGc                  <  1:1424116/151‑1 (MQ=255)
                                                                                                                                 gCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGTTAGcc                 <  2:491156/150‑1 (MQ=255)
                                                                                                                                 gCCCTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGTTAGCCa                <  1:1374752/151‑1 (MQ=255)
                                                                                                                                  cccTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGTTAGcc                 >  1:1047207/1‑149 (MQ=255)
                                                                                                                                  cccTCCTCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGTTAGCCa                <  2:41802/150‑1 (MQ=255)
                                                                                                                                   cctcctCAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGTTAGCCAgg              <  1:608625/151‑1 (MQ=255)
                                                                                                                                     tcctcAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGTTAGCCAgg              >  1:389928/1‑149 (MQ=255)
                                                                                                                                       ctcAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGTTAGCCAGGAGCt          >  2:250435/1‑151 (MQ=255)
                                                                                                                                         cAATCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGTTAGCCAGGAGCt          <  2:471230/149‑1 (MQ=255)
                                                                                                                                           aTCGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGTTAGCCAGGAGCTCAgg      >  1:13006/1‑151 (MQ=255)
                                                                                                                                             cGGACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGTTAGCCAGGAGCTCAGGa     <  1:1435260/150‑1 (MQ=255)
                                                                                                                                               gACGTAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGTTAGCCAGGAGCTCAGGAAAt  <  1:542632/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTCGAACATGGCATGCCCTCCTCAATCGGACATAGCCGCCGGGTACAGCTGCGATCCATCCTGCTAACTCCAGTTCGAGTAGTTGAGTAACTACCTCTGGCACAGGTTGGCCGGCACGTTCAGCGACGACGTCAACAGGTGTTACCTCATCTCCTACGTTAGCCAGGAGCTCAGGAAAT  >  NC_000913/3432341‑3432634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A