Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,132,321	C→T	T156T (ACC→ACT)	flgD →	flagellar hook assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,132,321	0	C	T	100.0%	176.4 / NA	44	T156T (ACC→ACT)	flgD	flagellar hook assembly protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/23); total (21/23)

TGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACCGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGG  >  NC_000913/1132177‑1132464

tGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGActgccg                                                                                                                                             <  1:20529/149‑1 (MQ=255)

 gCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCgg                                                                                                                                            >  2:272405/1‑149 (MQ=255)

    ggAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTc                                                                                                                                       >  1:46151/1‑151 (MQ=255)

       aCCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTcaca                                                                                                                                    <  2:207337/151‑1 (MQ=255)

          ggCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGtt                                                                                                                                 <  1:577760/151‑1 (MQ=255)

                     agGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGAc                                                                                                                      <  1:316604/151‑1 (MQ=255)

                               accacgaccacgCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTg                                                                                                             >  1:590831/1‑150 (MQ=255)

                                ccacgaccacgCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGAc                                                                                                           >  1:440888/1‑151 (MQ=255)

                                   cgaccacgCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTg                                                                                                         >  1:661777/1‑150 (MQ=255)

                                          gCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGgcagc                                                                                                 <  1:571774/151‑1 (MQ=255)

                                           ccGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGgcagca                                                                                                <  2:506185/151‑1 (MQ=255)

                                                 ggTGTTGGGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTgcgc                                                                                          <  1:261006/151‑1 (MQ=255)

                                                      tGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAAc                                                                                     >  2:389838/1‑151 (MQ=255)

                                                       gAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACg                                                                                    <  2:353280/151‑1 (MQ=255)

                                                       gAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACg                                                                                    <  2:398012/151‑1 (MQ=255)

                                                       gAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCAATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAAc                                                                                     <  2:350261/150‑1 (MQ=255)

                                                             cAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCtt                                                                              <  1:674539/151‑1 (MQ=255)

                                                             cAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCtt                                                                              <  2:570745/151‑1 (MQ=255)

                                                             cAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACATTGACTGATGGCAGCACTGCGCCGAACGGTTCtt                                                                              <  1:4275/151‑1 (MQ=255)

                                                                 aGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACa                                                                           >  2:319690/1‑150 (MQ=255)

                                                                  ggcggcAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAAt                                                                         <  1:164043/151‑1 (MQ=255)

                                                                       cAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGc                                                                    <  1:590705/151‑1 (MQ=255)

                                                                        aGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCg                                                                   <  2:265064/151‑1 (MQ=255)

                                                                           cAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGAtt                                                                >  1:573633/1‑151 (MQ=255)

                                                                             aaGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAg                                                              <  1:319314/151‑1 (MQ=255)

                                                                              aGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAgc                                                             <  2:389012/151‑1 (MQ=255)

                                                                              aGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAgc                                                             <  1:389849/151‑1 (MQ=255)

                                                                                     gCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGt                                                       >  1:488004/1‑150 (MQ=255)

                                                                                        accaTCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACg                                                   <  2:442183/151‑1 (MQ=255)

                                                                                         ccaTCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACgg                                                  >  1:677878/1‑151 (MQ=255)

                                                                                           aTCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACggtg                                                >  2:263331/1‑151 (MQ=255)

                                                                                              aCCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACggtggt                                              >  1:28605/1‑150 (MQ=255)

                                                                                                 gATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTacac                                          >  1:670142/1‑151 (MQ=255)

                                                                                                   tAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAGCGGTGGTAcaca                                         >  2:153580/1‑150 (MQ=255)

                                                                                                      aaaTGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTg                                     >  1:595847/1‑151 (MQ=255)

                                                                                                        aTGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGt                                   <  1:171848/151‑1 (MQ=255)

                                                                                                        aTGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGt                                   <  2:552316/151‑1 (MQ=255)

                                                                                                                   gtgCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGc                        >  2:458926/1‑151 (MQ=255)

                                                                                                                        cACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAg                   <  1:594576/151‑1 (MQ=255)

                                                                                                                                    tGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTg       >  2:176936/1‑151 (MQ=255)

                                                                                                                                     ggTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGc      <  2:421724/151‑1 (MQ=255)

                                                                                                                                       tGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGc      >  2:612635/1‑149 (MQ=255)

                                                                                                                                        gAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAgg   >  2:335177/1‑151 (MQ=255)

                                                                                                                                        aaaCTGACTGCCGGAGATCACAGTTTCACCAAGGACGGTACGTTGACTGATAGCAGCACTGCGCAGAACGGTTCTTACAATGTAGCGATTAGCGCCAGAAACGGAGGTACACAACTGGTTCTCCAGCCGCAGCATCGTGCTCTGGTGCAgg   >  2:545750/2‑151 (MQ=255)

                                                                                                                                         aaCTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAggg  >  2:627343/1‑151 (MQ=255)

                                                                                                                                          actgactgCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGt                                                       >  1:13015/1‑97 (MQ=255)

                                                                                                                                          actgactgCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGt                                                       <  2:13015/97‑1 (MQ=255)

TGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACCGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGG  >  NC_000913/1132177‑1132464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A