Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,805,310	T→C	S166P (TCT→CCT)	proV →	glycine betaine/proline ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,805,310	0	T	C	100.0%	140.6 / NA	37	S166P (TCT→CCT)	proV	glycine betaine/proline ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/18); total (19/18)

CTTAATGCCGCATATGACCGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAGGCGAAAC  >  NC_000913/2805162‑2805454

cTTAATGCCGCATATGACCGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAActccct                                                                                                                                                >  2:304946/1‑151 (MQ=255)

 ttAATGCCGCATATGACCGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTg                                                                                                                                               >  2:39894/1‑151 (MQ=255)

          cATATGACCGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGc                                                                                                                                      <  1:216117/151‑1 (MQ=255)

                aCCGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAAc                                                                                                                                >  2:418651/1‑151 (MQ=255)

                aCCGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAAc                                                                                                                                >  1:327299/1‑151 (MQ=255)

                  cGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCTGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGTGATGCGTCAAc                                                                                                                                >  2:385702/1‑149 (MQ=255)

                              tACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGcc                                                                                                                  >  2:581618/1‑151 (MQ=255)

                                 tGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCcgc                                                                                                               >  1:127692/1‑151 (MQ=255)

                                   cGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCcgcgc                                                                                                             <  1:478416/151‑1 (MQ=255)

                                    gTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCcgcgcg                                                                                                            <  1:278769/151‑1 (MQ=255)

                                       cGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGAGCGtt                                                                                                          >  2:255289/1‑150 (MQ=255)

                                         gTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGc                                                                                                       <  1:490451/151‑1 (MQ=255)

                                                  tGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATc                                                                                               <  1:426740/150‑1 (MQ=255)

                                                    gCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCgg                                                                                            >  2:48048/1‑151 (MQ=255)

                                                       ggAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGata                                                                                         <  1:359789/151‑1 (MQ=255)

                                                       ggAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGat                                                                                          >  1:546198/1‑150 (MQ=255)

                                                              aTGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATattatt                                                                                  >  1:289497/1‑151 (MQ=255)

                                                                gCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTaa                                                                                <  2:505660/151‑1 (MQ=255)

                                                                  cGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATg                                                                              <  2:19997/151‑1 (MQ=255)

                                                                    aagaaCGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATgg                                                                             >  1:459646/1‑150 (MQ=255)

                                                                            cGGGAAAAAGCACTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGAAGCGTCAACCCGTGGGATTAGCCTGCGCGTTAGCGATTAATCCGGAAATATTATTAATGGACGa                                                                         >  2:334732/1‑146 (MQ=255)

                                                                                   aaGCCCTTGATGCACTGCGTCAGGTGGGGCTGGAAAATTATGCCCACGGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGgc                                                             <  2:399080/151‑1 (MQ=255)

                                                                                   aaGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGgc                                                             >  1:494284/1‑151 (MQ=255)

                                                                                    aGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGgcg                                                            <  2:639331/151‑1 (MQ=255)

                                                                                           gATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTAGa                                                       <  1:465005/149‑1 (MQ=255)

                                                                                                      tCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAAAGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCg                                           >  2:221460/1‑150 (MQ=255)

                                                                                                       cAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCa                                         <  1:311608/151‑1 (MQ=255)

                                                                                                            cGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCga                                     <  1:474948/150‑1 (MQ=255)

                                                                                                            cGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTCGGATTAGCCCGCGCGTTAGCGATTATTCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCg                                      >  2:601925/1‑149 (MQ=255)

                                                                                                                   gAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAgg                             <  1:401213/151‑1 (MQ=255)

                                                                                                                   gAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAgg                             <  2:533300/151‑1 (MQ=255)

                                                                                                                   gAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAgg                             <  1:304953/151‑1 (MQ=255)

                                                                                                                            gCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTgg                    >  2:609120/1‑151 (MQ=255)

                                                                                                                               cacaGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTaa                 <  1:441415/151‑1 (MQ=255)

                                                                                                                                acaGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTaa                 >  2:125707/1‑150 (MQ=255)

                                                                                                                                     tACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTAc           >  1:80273/1‑151 (MQ=255)

                                                                                                                                         cGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAGGc       <  1:84376/151‑1 (MQ=255)

                                                                                                                                              gAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAGGCGAAAc  >  1:481881/1‑151 (MQ=255)

CTTAATGCCGCATATGACCGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAGGCGAAAC  >  NC_000913/2805162‑2805454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A