Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,368,566	G→A	G247E (GGG→GAG)	yhcG →	DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,368,566	0	G	A	100.0%	173.1 / NA	45	G247E (GGG→GAG)	yhcG	DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/20); total (25/20)

CCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGGGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATG  >  NC_000913/3368424‑3368709

ccTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGgatga                                                                                                                                           <  2:306844/149‑1 (MQ=255)

ccTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGAtt                                                                                                                                         >  2:303209/1‑151 (MQ=255)

ccTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGAtt                                                                                                                                         >  2:497592/1‑151 (MQ=255)

   gCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTg                                                                                                                                      <  1:110942/151‑1 (MQ=255)

      gagaCGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCt                                                                                                                                   >  2:568867/1‑151 (MQ=255)

             atatTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTgg                                                                                                                            <  1:509983/151‑1 (MQ=255)

                     ccGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGc                                                                                                                    <  1:493528/151‑1 (MQ=255)

                                gATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGc                                                                                                         <  2:591677/151‑1 (MQ=255)

                                    cgcgATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATtg                                                                                                     >  1:327095/1‑151 (MQ=255)

                                     gcgATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATtga                                                                                                    <  1:220308/151‑1 (MQ=255)

                                       gATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGTATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATtgatg                                                                                                  <  1:412994/151‑1 (MQ=255)

                                       gATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATtgatg                                                                                                  >  2:465934/1‑151 (MQ=255)

                                         tCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGAc                                                                                                >  1:152077/1‑151 (MQ=255)

                                          cccTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGAc                                                                                                >  2:427026/1‑150 (MQ=255)

                                           ccTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACa                                                                                               >  2:195042/1‑150 (MQ=255)

                                              ttGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACt                                                                                            <  2:277193/150‑1 (MQ=255)

                                               tgtgCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTg                                                                                           <  2:33474/150‑1 (MQ=255)

                                                    tCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCg                                                                                     >  1:165155/1‑151 (MQ=255)

                                                     cGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCg                                                                                     >  2:311748/1‑150 (MQ=255)

                                                       aaTTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCgtgt                                                                                  >  1:602233/1‑151 (MQ=255)

                                                              ggAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGAt                                                                              <  1:481754/148‑1 (MQ=255)

                                                              ggAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATctg                                                                           >  1:154026/1‑151 (MQ=255)

                                                                aaTTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATctgct                                                                         >  1:105142/1‑151 (MQ=255)

                                                                      aaGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGttttt                                                                   >  1:270780/1‑151 (MQ=255)

                                                                            aaTATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCAccg                                                             <  2:294476/151‑1 (MQ=255)

                                                                                ttCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGt                                                         >  2:512714/1‑151 (MQ=255)

                                                                                  cAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGttt                                                       >  2:460253/1‑151 (MQ=255)

                                                                                   aGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTa                                                      <  1:499361/151‑1 (MQ=255)

                                                                                        ccGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGctg                                                 >  1:275247/1‑151 (MQ=255)

                                                                                         cGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGctgc                                                >  1:588855/1‑151 (MQ=255)

                                                                                              ttGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGctgcctgc                                            <  2:93739/150‑1 (MQ=255)

                                                                                                gaggagGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTa                                          <  1:662067/150‑1 (MQ=255)

                                                                                                          tGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCt                               >  1:662817/1‑151 (MQ=255)

                                                                                                           gATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATGGATGACAACTGGTTTCTCGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCt                               <  2:130518/150‑1 (MQ=255)

                                                                                                                  caccTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTgg                       <  2:66602/151‑1 (MQ=255)

                                                                                                                   accTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTggg                      >  1:395978/1‑151 (MQ=255)

                                                                                                                    ccTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTggg                      <  2:638039/150‑1 (MQ=255)

                                                                                                                       gATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCaa                   >  1:383437/1‑150 (MQ=255)

                                                                                                                        aTGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAAt                 >  1:678452/1‑151 (MQ=255)

                                                                                                                          ggATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGTCAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTc               >  2:161924/1‑151 (MQ=255)

                                                                                                                              ttCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCt           >  1:136041/1‑151 (MQ=255)

                                                                                                                                cATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCtat         <  1:497419/151‑1 (MQ=255)

                                                                                                                                 aTGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCtata        <  2:153027/151‑1 (MQ=255)

                                                                                                                                 aTGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCtata        <  2:327088/151‑1 (MQ=255)

                                                                                                                                       gAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATg  >  1:551907/1‑151 (MQ=255)

CCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGGGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATG  >  NC_000913/3368424‑3368709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A