Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F50 I2 R1 104 44.1 1376607 98.9% 1361464 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,370,354 A→G V228A (GTG→GCG)  nanE ← putative N‑acetylmannosamine‑6‑P epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,370,3540AG100.0% 144.1 / NA 40V228A (GTG→GCG) nanEputative N‑acetylmannosamine‑6‑P epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/14);  total (26/14)

AATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACC  >  NC_000913/3370208‑3370497
                                                                                                                                                  |                                                                                                                                               
aaTGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGcgccgc                                                                                                                                             >  1:199436/1‑151 (MQ=255)
 aTGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCgccgcc                                                                                                                                            >  1:153616/1‑151 (MQ=255)
  tGCGGATAAGGCATCACGCAGGGCTTCGGGTGTCTGGCGGGCTGGCGTAGGAAGTTCCCGACGATCGGGGATCTCCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCgccgcc                                                                                                                                            <  2:292226/150‑1 (MQ=255)
   gCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCtt                                                                                                                                          >  2:438197/1‑151 (MQ=255)
     cgATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGAAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGACGATATCAATCGCCAGTGTGGTCATAGCGCCGCCtttt                                                                                                                                        >  2:54317/2‑151 (MQ=255)
      gATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCttttt                                                                                                                                       >  1:330826/1‑151 (MQ=255)
             cATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCt                                                                                                                                >  2:584126/1‑151 (MQ=255)
                                  tCTGGTTGGCTGGAGTAGGAAGTCCCCGCCGATCGCGTATCTGCCCGGCAGCGCCAATCGGGGCGGGGGCGATTTTTTTGCCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAAt                                                                                                            <  1:514064/150‑1 (MQ=255)
                                     ggcgggctgggGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAACCGCCGCGGCGGCAAGTTTTTTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACaa                                                                                                              <  1:369677/145‑1 (MQ=255)
                                                   ggAAGTTCGCGGCGATCGCGGATCTGCCCATCAGCGCAAATCGGCGCGGGGGAAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGAcgcg                                                                                              <  2:270656/147‑1 (MQ=255)
                                                        ttCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGc                                                                                       >  2:30488/1‑149 (MQ=255)
                                                           acgacgATCGCGGATCTGCCCGGCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGaa                                                                                   <  1:627799/150‑1 (MQ=255)
                                                             gacgaTCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAacc                                                                                 >  2:264377/1‑150 (MQ=255)
                                                                gaTCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAaccgacc                                                                             >  2:275509/1‑151 (MQ=255)
                                                                    gcgGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTc                                                                          >  2:468595/1‑150 (MQ=255)
                                                                    gcgGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTc                                                                          >  1:2078/1‑150 (MQ=255)
                                                                      ggATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCAcc                                                                       >  1:68897/1‑151 (MQ=255)
                                                                                gTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACgcgc                                                             >  1:13744/1‑151 (MQ=255)
                                                                                 tCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGcc                                                            >  1:540866/1‑151 (MQ=255)
                                                                                   aGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGt                                                          >  2:21531/1‑151 (MQ=255)
                                                                                         aaTCAGCGGGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgca                                                    <  2:578058/151‑1 (MQ=255)
                                                                                          accAGCGGGGCGGAAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgcat                                                   <  2:488928/149‑1 (MQ=255)
                                                                                              gcgcGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgcatcgc                                                >  1:611541/1‑150 (MQ=255)
                                                                                               cgcgGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgcatcgcat                                              >  1:325572/1‑151 (MQ=255)
                                                                                                  ggcggcAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATccg                                           >  2:356844/1‑151 (MQ=255)
                                                                                                  ggcggcAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATcc                                            <  2:459177/150‑1 (MQ=255)
                                                                                                     ggcAAGTTTATTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATccgcc                                         <  2:188200/150‑1 (MQ=255)
                                                                                                     ggcAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATccgccg                                        >  2:201765/1‑151 (MQ=255)
                                                                                                     ggaaaGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATccgccg                                        <  2:509109/148‑1 (MQ=255)
                                                                                                         attttAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCgccgcc                                      <  2:28260/147‑1 (MQ=255)
                                                                                                          gTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTg                                    >  2:262875/1‑150 (MQ=255)
                                                                                                          gTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTg                                    >  1:670947/1‑150 (MQ=255)
                                                                                                           tttAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCt                                     >  2:597391/1‑148 (MQ=255)
                                                                                                           tttAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTg                                    >  1:456322/1‑149 (MQ=255)
                                                                                                                tACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGc                             >  1:487908/1‑151 (MQ=255)
                                                                                                                  ccgccgATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCgt                           <  2:218074/151‑1 (MQ=255)
                                                                                                                   cgccgATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCgtg                          <  1:677003/151‑1 (MQ=255)
                                                                                                                       gATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGtgttgt                      >  1:41464/1‑151 (MQ=255)
                                                                                                                          atCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAAc                   >  1:233528/1‑151 (MQ=255)
                                                                                                                                cGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCtt             <  2:330832/151‑1 (MQ=255)
                                                                                                                                 gCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGACCCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTc            <  2:684017/151‑1 (MQ=255)
                                                                                                                                     gtgtGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGc         >  2:113044/1‑150 (MQ=255)
                                                                                                                                           tCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCAcc  >  1:408236/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
AATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACC  >  NC_000913/3370208‑3370497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A