Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F50 I2 R1
|
209 |
44.2 |
1360995 |
98.7% |
1343302 |
150.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,532,256 |
+T |
coding (184/1725 nt) |
yhgE ← |
DUF4153 family putative inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,532,253 | 1 | . | T | 84.4%
| 145.2
/ 21.7
| 45 | coding (187/1725 nt) | yhgE | DUF4153 family putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (4/3); new base T (23/15); total (27/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 |
CATTGCCATCAACAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAGACCCTGAAGTAATCCGGT > NC_000913/3532104‑3532391
|
caTTGCCATCAACAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑t > 2:400945/1‑151 (MQ=255)
tGCCATCAACAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑ttt < 1:408134/150‑1 (MQ=255)
ccATCAACAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGGCAGCCATCCCCAAAGGCGCTTCTG‑TTTtaa < 1:524951/151‑1 (MQ=255)
cATCAACAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTtaaa < 2:126132/151‑1 (MQ=255)
aTCAACAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTtaaa > 1:94133/1‑150 (MQ=255)
aaCAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCTCTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAagag < 2:165307/151‑1 (MQ=255)
aaCAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAagag < 2:68985/151‑1 (MQ=255)
aaCAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTtaaaag > 1:673665/1‑147 (MQ=255)
aCAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAgaga < 2:452455/151‑1 (MQ=255)
agcagATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTtaaaag > 1:664043/1‑144 (MQ=255)
tAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACgg > 2:73653/1‑149 (MQ=255)
aaCCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGgaga > 1:197317/1‑148 (MQ=255)
ccAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGt > 1:151492/1‑151 (MQ=255)
aaaaTCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTaa < 1:218103/151‑1 (MQ=255)
aaaTCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACAGAGAAGAGTaa > 2:520810/1‑150 (MQ=255)
aaTCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTaaaa < 2:563737/151‑1 (MQ=255)
gcCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAAc > 2:286135/1‑151 (MQ=255)
cAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACgg > 1:286288/1‑151 (MQ=255)
cAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACg < 2:178298/150‑1 (MQ=255)
aGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTtaaa < 2:510431/109‑1 (MQ=255)
aGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTtaaa > 1:510445/1‑109 (MQ=255)
ctcCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGc > 2:191799/1‑150 (MQ=255)
aGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAgg > 2:619095/1‑149 (MQ=255)
aGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGc > 1:522625/1‑150 (MQ=255)
gggTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACg < 1:79897/151‑1 (MQ=255)
aTGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATa > 2:645367/1‑150 (MQ=255)
cATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACa > 1:248585/1‑150 (MQ=255)
aTCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAAt > 2:512091/1‑151 (MQ=255)
ttGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTAGCAGGCACGCCATAAACAATCCAACt > 2:244380/1‑151 (MQ=255)
aCTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGAt > 1:603236/1‑151 (MQ=255)
agccagccGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGAtttttt < 1:653739/151‑1 (MQ=255)
ccagccGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTcc < 1:220323/151‑1 (MQ=255)
cGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGaa > 2:201663/1‑150 (MQ=255)
tCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGcc > 1:518748/1‑151 (MQ=255)
aGCGGGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGAt < 2:393408/150‑1 (MQ=255)
cAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAgg > 2:498455/1‑150 (MQ=255)
cAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGt < 1:269441/151‑1 (MQ=255)
aaaCACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCa < 2:664022/151‑1 (MQ=255)
cTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAAcc > 2:620416/1‑150 (MQ=255)
aGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAAcc > 1:173970/1‑148 (MQ=255)
aGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCa < 1:45884/149‑1 (MQ=255)
aGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAg < 2:601722/150‑1 (MQ=255)
cgcCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAGAcc > 2:87001/1‑151 (MQ=255)
cagccaTCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAGACCCTg > 1:214590/1‑151 (MQ=255)
ccccAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAGACCCTGAAGTaa < 1:216102/150‑1 (MQ=255)
aaaGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAGACCCTGAAGTAATCCgg > 2:421059/1‑151 (MQ=255)
aGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAGACCCTGAAGTAATCCGGt > 1:75681/1‑150 (MQ=255)
|
CATTGCCATCAACAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAGACCCTGAAGTAATCCGGT > NC_000913/3532104‑3532391
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
