Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,537,161	A→G	Y117C (TAC→TGC)	greB →	transcript cleavage factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,537,161	0	A	G	100.0%	158.1 / NA	42	Y117C (TAC→TGC)	greB	transcript cleavage factor
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/25); total (17/25)

GCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACC  >  NC_000913/3537014‑3537303

gCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCa                                                                                                                                              <  2:90759/150‑1 (MQ=255)

      aaaaTCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTAt                                                                                                                                       <  2:346781/151‑1 (MQ=255)

        aaTCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCg                                                                                                                                     <  1:443742/151‑1 (MQ=255)

          tctcAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGAt                                                                                                                                   >  1:597857/1‑151 (MQ=255)

             cAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTcc                                                                                                                                >  1:102642/1‑151 (MQ=255)

                       gATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCTCCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATTGccc                                                                                                                      <  2:141070/151‑1 (MQ=255)

                        aTTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCcg                                                                                                                     >  1:164529/1‑151 (MQ=255)

                           aCTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCgcgc                                                                                                                  >  1:272597/1‑151 (MQ=255)

                            cTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGAt                                                                                                                           <  1:33105/141‑1 (MQ=255)

                            cTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGAt                                                                                                                           >  2:33105/1‑141 (MQ=255)

                                ccTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGc                                                                                                             <  1:474519/151‑1 (MQ=255)

                                   cagcagGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTg                                                                                                           <  1:578340/150‑1 (MQ=255)

                                     gcagGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGaaa                                                                                                        <  1:522498/151‑1 (MQ=255)

                                        ggAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGaaaaaa                                                                                                     <  2:272590/151‑1 (MQ=255)

                                             gCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGt                                                                                                >  2:500867/1‑151 (MQ=255)

                                               aaaGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTc                                                                                               <  1:583226/150‑1 (MQ=255)

                                               aaaGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCg                                                                                              <  2:124494/151‑1 (MQ=255)

                                                        tttGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTg                                                                                      <  2:390309/150‑1 (MQ=255)

                                                          tGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCg                                                                                   >  1:572126/1‑151 (MQ=255)

                                                               cgTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCggtgg                                                                               >  2:135307/1‑150 (MQ=255)

                                                                    gtggAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAAt                                                                          >  1:170382/1‑150 (MQ=255)

                                                                       gagaTTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATAccc                                                                      >  1:48323/1‑151 (MQ=255)

                                                                       gagaTTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATAccc                                                                      <  2:70632/151‑1 (MQ=255)

                                                                         gaTTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCt                                                                    <  2:182261/151‑1 (MQ=255)

                                                                          aTTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTg                                                                   >  2:289285/1‑151 (MQ=255)

                                                                               aaaCGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCggg                                                              <  2:613810/151‑1 (MQ=255)

                                                                                  cgacgaTGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGaa                                                           <  2:161946/151‑1 (MQ=255)

                                                                                   gacgaTGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGa                                                            >  1:130124/1‑149 (MQ=255)

                                                                                          gCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTg                                                   <  2:249045/151‑1 (MQ=255)

                                                                                           cGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTgg                                                  >  2:310632/1‑151 (MQ=255)

                                                                                            gTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGt                                                 <  1:135310/151‑1 (MQ=255)

                                                                                              gACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTa                                                <  2:375047/150‑1 (MQ=255)

                                                                                               aCTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATg                                              <  2:630304/151‑1 (MQ=255)

                                                                                               aCTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATg                                              >  1:279277/1‑151 (MQ=255)

                                                                                                     cGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATg                                        <  2:406890/151‑1 (MQ=255)

                                                                                                         tCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTAt                                    >  2:77341/1‑151 (MQ=255)

                                                                                                               ttGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGt                               >  2:4552/1‑150 (MQ=255)

                                                                                                               ttGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGt                               <  1:309796/150‑1 (MQ=255)

                                                                                                                      cTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGaa                        >  1:19895/1‑150 (MQ=255)

                                                                                                                                aTTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGaa             <  2:19895/151‑1 (MQ=255)

                                                                                                                                         cGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTaa    <  1:509360/151‑1 (MQ=255)

                                                                                                                                            aaaGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAAcc  <  2:331924/150‑1 (MQ=255)

GCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACC  >  NC_000913/3537014‑3537303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A