Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,546,644	T→C	V29A (GTC→GCC)	gntT →	gluconate transporter, high‑affinity GNT I system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,546,644	0	T	C	100.0%	184.6 / NA	53	V29A (GTC→GCC)	gntT	gluconate transporter, high‑affinity GNT I system
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/26); total (27/26)

AATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGA  >  NC_000913/3546498‑3546788

aaTTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTc                                                                                                                                              <  1:55061/151‑1 (MQ=255)

 aTTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCg                                                                                                                                             <  1:176709/151‑1 (MQ=255)

    agagTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTg                                                                                                                                           <  2:396378/150‑1 (MQ=255)

       gTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGgcgc                                                                                                                                       >  2:562681/1‑151 (MQ=255)

        tttGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCt                                                                                                                                      >  1:213448/1‑151 (MQ=255)

        tttGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCt                                                                                                                                      >  2:295838/1‑151 (MQ=255)

          tGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCtt                                                                                                                                     <  1:460936/150‑1 (MQ=255)

          tGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTg                                                                                                                                    >  2:536398/1‑151 (MQ=255)

             cgtcAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTg                                                                                                                                 >  2:325077/1‑151 (MQ=255)

               tcAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTg                                                                                                                                 <  1:231732/149‑1 (MQ=255)

                cAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTg                                                                                                                              >  1:622411/1‑151 (MQ=255)

                  gAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTgg                                                                                                                             >  1:209011/1‑150 (MQ=255)

                  gAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGa                                                                                                                            <  2:367605/151‑1 (MQ=255)

                    aaaTTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGAtt                                                                                                                          >  1:412791/1‑151 (MQ=255)

                     aaTTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGAtt                                                                                                                          >  1:528014/1‑150 (MQ=255)

                         gACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATg                                                                                                                      <  1:32546/150‑1 (MQ=255)

                           cGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCaa                                                                                                                   <  2:161816/151‑1 (MQ=255)

                              tACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAgg                                                                                                                 <  1:79126/150‑1 (MQ=255)

                                    tAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGcc                                                                                                           >  2:670089/1‑150 (MQ=255)

                                     aaCAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGc                                                                                                         >  2:450947/1‑151 (MQ=255)

                                        aaaTGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTgg                                                                                                      >  2:458739/1‑151 (MQ=255)

                                            gAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATa                                                                                                   <  2:127645/150‑1 (MQ=255)

                                              aaGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATaa                                                                                                  >  1:212362/1‑149 (MQ=255)

                                              aaGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATaa                                                                                                  >  2:301907/1‑149 (MQ=255)

                                                 gCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGtt                                                                                              >  1:358857/1‑150 (MQ=255)

                                                  ccAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTAt                                                                                            >  2:608136/1‑151 (MQ=255)

                                                       tAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCt                                                                                       >  1:674578/1‑151 (MQ=255)

                                                        aaaTCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTc                                                                                      >  2:655579/1‑151 (MQ=255)

                                                            cATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCAt                                                                                   <  2:387125/150‑1 (MQ=255)

                                                             aTGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCa                                                                                 <  1:655600/151‑1 (MQ=255)

                                                              tGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCa                                                                                 >  2:601973/1‑150 (MQ=255)

                                                               gCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCaaa                                                                               <  1:627916/151‑1 (MQ=255)

                                                                    tAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCg                                                                           <  2:5434/150‑1 (MQ=255)

                                                                              tgCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTcggcgg                                                                 <  1:126303/150‑1 (MQ=255)

                                                                              tgCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCggg                                                                >  2:366772/1‑151 (MQ=255)

                                                                               gCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCggg                                                                <  1:475897/150‑1 (MQ=255)

                                                                                  aTCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGAc                                                              <  1:587274/149‑1 (MQ=255)

                                                                                  aTCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGc                                                            <  1:252651/151‑1 (MQ=255)

                                                                                        gtACTCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGAc                                                              >  2:138993/1‑143 (MQ=255)

                                                                                                  tgttgCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGccc                                             >  2:5281/1‑150 (MQ=255)

                                                                                                             atgatCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGtt                                 <  2:213443/151‑1 (MQ=255)

                                                                                                              tgatCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGttt                                >  2:190693/1‑151 (MQ=255)

                                                                                                               gatCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGtttt                               <  1:677708/151‑1 (MQ=255)

                                                                                                               gatCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGttt                                <  2:388581/150‑1 (MQ=255)

                                                                                                                       tCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                         <  2:115132/149‑1 (MQ=255)

                                                                                                                              gAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                >  2:34987/1‑151 (MQ=255)

                                                                                                                                  ggCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa            <  2:358847/151‑1 (MQ=255)

                                                                                                                                     ttCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc         >  2:411558/1‑151 (MQ=255)

                                                                                                                                     ttCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc         >  2:592031/1‑151 (MQ=255)

                                                                                                                                       cATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg       <  2:444286/151‑1 (MQ=255)

                                                                                                                                        aTCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg      <  2:32624/151‑1 (MQ=255)

                                                                                                                                         tCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCTGGGCAAAATGCTgg      >  2:192792/1‑150 (MQ=255)

                                                                                                                                              ctcGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGa  <  2:530135/149‑1 (MQ=255)

AATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGA  >  NC_000913/3546498‑3546788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A