Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,689,711	G→A	P184L (CCG→CTG)	bcsZ ←	endo‑1,4‑D‑glucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,689,711	0	G	A	100.0%	169.0 / NA	45	P184L (CCG→CTG)	bcsZ	endo‑1,4‑D‑glucanase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/28); total (17/28)

AGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAA  >  NC_000913/3689567‑3689854

aGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAgg                                                                                                                                            <  2:501428/150‑1 (MQ=255)

 gCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTa                                                                                                                                          <  2:374904/151‑1 (MQ=255)

  cTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGt                                                                                                                                           >  2:415713/1‑149 (MQ=255)

    gCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGc                                                                                                                                        >  1:126193/1‑150 (MQ=255)

      cAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTgg                                                                                                                                     <  2:493571/151‑1 (MQ=255)

           tttGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGtt                                                                                                                                 >  1:327037/1‑150 (MQ=255)

                cTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGCTTCCAGAAATAAACGTTGATTGGTTTCACACAGCGTGGACCACGGCGCGCCAAAGCGGGCGTAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAAc                                                                                                                            >  1:585970/1‑150 (MQ=255)

                   tctcATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGATTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGcc                                                                                                                         >  1:552824/1‑150 (MQ=255)

                    ctcATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGcc                                                                                                                         >  2:468762/1‑149 (MQ=255)

                       aTAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTg                                                                                                                    <  1:51518/151‑1 (MQ=255)

                          gcgcACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTa                                                                                                                 <  1:516773/151‑1 (MQ=255)

                              aCCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATcc                                                                                                              >  2:472998/1‑150 (MQ=255)

                              aCCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGGTTCGCGCAGCGTGGTGCACGGCCCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACCCCAGCTGTTATcc                                                                                                              >  1:358798/1‑150 (MQ=255)

                               cccAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTc                                                                                                            <  1:482574/151‑1 (MQ=255)

                                ccAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCa                                                                                                           <  2:660931/151‑1 (MQ=255)

                                      tGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCaaa                                                                                                      >  2:97516/1‑150 (MQ=255)

                                                         gCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTaaca                                                                                  <  1:73080/151‑1 (MQ=255)

                                                              ttCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATg                                                                             >  2:157284/1‑151 (MQ=255)

                                                               tCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATg                                                                             <  1:573476/150‑1 (MQ=255)

                                                               tCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATg                                                                             <  1:324238/150‑1 (MQ=255)

                                                                     aaTAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAAccc                                                                       >  1:316308/1‑150 (MQ=255)

                                                                        aaaCGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAAcccagcc                                                                   <  2:474602/151‑1 (MQ=255)

                                                                        aaaCGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAAcccagc                                                                    <  2:197812/150‑1 (MQ=255)

                                                                              tGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGCGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGc                                                              >  1:623155/1‑150 (MQ=255)

                                                                                aTTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCAcc                                                           <  2:485609/151‑1 (MQ=255)

                                                                                          cgcAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACtt                                                 <  2:377009/151‑1 (MQ=255)

                                                                                           gcAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACtt                                                 <  2:140472/150‑1 (MQ=255)

                                                                                             aGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTtcct                                              >  1:25227/1‑151 (MQ=255)

                                                                                             aGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTtcc                                               <  1:395755/150‑1 (MQ=255)

                                                                                                gTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTccc                                           <  2:497553/151‑1 (MQ=255)

                                                                                                  ggTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCcgc                                         <  1:406106/151‑1 (MQ=255)

                                                                                                     ccACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCcgcgc                                       <  1:464009/150‑1 (MQ=255)

                                                                                                       aCGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGAt                                    <  2:99335/151‑1 (MQ=255)

                                                                                                        cGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGAt                                    >  1:553338/1‑150 (MQ=255)

                                                                                                        cGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATa                                   <  2:224115/151‑1 (MQ=255)

                                                                                                           cgcgcCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGt                                <  1:159683/151‑1 (MQ=255)

                                                                                                                cAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGtttt                             >  1:29117/1‑149 (MQ=255)

                                                                                                                     cGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAAcg                      >  1:395625/1‑151 (MQ=255)

                                                                                                                          gAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCt                  <  1:638816/150‑1 (MQ=255)

                                                                                                                             aTACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCg              <  2:673533/151‑1 (MQ=255)

                                                                                                                                cTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCg              >  2:501182/1‑148 (MQ=255)

                                                                                                                                  gcgcCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCATCAAAACCCTCTTTGCCCGGTATCAACATGGAACCCAGCCCAGGCCCCGTCACCTCTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTc         <  2:527723/151‑1 (MQ=255)

                                                                                                                                    gcCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCgg       <  1:161927/151‑1 (MQ=255)

                                                                                                                                     ccAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGt      <  2:633215/151‑1 (MQ=255)

                                                                                                                                         cGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATaa  <  1:66656/151‑1 (MQ=255)

AGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAA  >  NC_000913/3689567‑3689854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A