Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,843,430	C→T	W120* (TGG→TGA)	adeQ ←	adenine permease, high affinity; adenine:H+ symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,843,430	0	C	T	100.0%	149.1 / NA	39	W120* (TGG→TGA)	adeQ	adenine permease, high affinity; adenine:H+ symporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/25); total (14/25)

CGACAATAACGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAA  >  NC_000913/3843282‑3843560

cGACAATAACGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCa                                                                                                                                  <  2:611023/151‑1 (MQ=255)

 gACAATAACGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAg                                                                                                                                 <  1:383591/151‑1 (MQ=255)

    aaTAACGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAat                                                                                                                              <  2:97907/151‑1 (MQ=255)

       aaCGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAatat                                                                                                                            <  1:615350/150‑1 (MQ=255)

         cGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTgc                                                                                                                         <  2:466099/151‑1 (MQ=255)

         cGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCCGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTgc                                                                                                                         >  2:554077/1‑151 (MQ=255)

              gTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGccc                                                                                                                     >  2:33350/1‑150 (MQ=255)

                aTTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATc                                                                                                                  >  2:548628/1‑151 (MQ=255)

                    tttAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCg                                                                                                               <  1:582229/150‑1 (MQ=255)

                     ttAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGt                                                                                                             >  1:95480/1‑151 (MQ=255)

                      tAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTc                                                                                                            >  1:360468/1‑151 (MQ=255)

                          cccATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGcc                                                                                                        >  2:428110/1‑151 (MQ=255)

                                aaGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGgaga                                                                                                  <  2:427723/151‑1 (MQ=255)

                                    cGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGcc                                                                                              >  2:208567/1‑151 (MQ=255)

                                     gATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGccc                                                                                             >  2:645182/1‑151 (MQ=255)

                                         aataatCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCg                                                                                         >  1:482452/1‑151 (MQ=255)

                                                 aaTTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCccacg                                                                                 <  2:100951/151‑1 (MQ=255)

                                                       gCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCccacgaccacg                                                                           >  1:219543/1‑151 (MQ=255)

                                                          ggTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCccacgaccacga                                                                          >  1:422304/1‑149 (MQ=255)

                                                             gATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCa                                                                     <  1:406731/151‑1 (MQ=255)

                                                              aTACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCaa                                                                    <  2:95478/151‑1 (MQ=255)

                                                                   aaTACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAaagaag                                                               <  2:536190/151‑1 (MQ=255)

                                                                   aaTACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAaagaag                                                               <  1:54363/151‑1 (MQ=255)

                                                                    aTACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAaagaag                                                               >  1:292356/1‑150 (MQ=255)

                                                                       cGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGt                                                           <  1:453466/151‑1 (MQ=255)

                                                                           aaCTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAg                                                       <  2:528831/151‑1 (MQ=255)

                                                                            aCTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGc                                                      <  2:669297/151‑1 (MQ=255)

                                                                            aCTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGc                                                      <  2:6954/151‑1 (MQ=255)

                                                                             cTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGcc                                                     >  2:372276/1‑151 (MQ=255)

                                                                                    gAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGc                                              >  1:664712/1‑151 (MQ=255)

                                                                                            gagaTCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCa                                      <  1:462374/151‑1 (MQ=255)

                                                                                                       gTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCAACGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGt                           <  1:433487/151‑1 (MQ=255)

                                                                                                         aCCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTaa                         <  2:173878/151‑1 (MQ=255)

                                                                                                          ccGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAg                        <  1:330793/151‑1 (MQ=255)

                                                                                                              aTACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAg                    <  2:68006/151‑1 (MQ=255)

                                                                                                                aCGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCa                  <  1:441113/151‑1 (MQ=255)

                                                                                                                             aGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCg     <  2:592435/151‑1 (MQ=255)

                                                                                                                              gCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGc    <  2:300735/151‑1 (MQ=255)

                                                                                                                                aaaaaTAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCaa  <  1:8946/151‑1 (MQ=255)

CGACAATAACGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAA  >  NC_000913/3843282‑3843560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A