Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,844,146	T→C	G61G (GGT→GGC)	adeD →	cryptic adenine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,844,146	0	T	C	100.0%	156.4 / NA	44	G61G (GGT→GGC)	adeD	cryptic adenine deaminase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/24); total (20/24)

TAGCCGGGCTGAATACCAGGAATTGTTAGCCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCC  >  NC_000913/3843999‑3844289

tAGCCGGGCTGAATACCAGGAATTGTTAGCCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGtt                                                                                                                                              >  1:207998/1‑151 (MQ=255)

                   gAATTGTTAGCCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATg                                                                                                                           <  2:80465/151‑1 (MQ=255)

                         ttAGCCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGgcgc                                                                                                                                         >  2:186198/1‑131 (MQ=255)

                         ttAGCCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGgcgc                                                                                                                                         <  1:186195/131‑1 (MQ=255)

                          tAGCCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCgg                                                                                                                     <  1:162012/150‑1 (MQ=255)

                                        ggCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGAtt                                                                                                       <  2:664680/150‑1 (MQ=255)

                                           gACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATg                                                                                                   >  1:279097/1‑151 (MQ=255)

                                              gCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTc                                                                                                >  2:460241/1‑151 (MQ=255)

                                                tGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTcgc                                                                                              >  1:308881/1‑151 (MQ=255)

                                                 gTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTcg                                                                                               <  2:271944/149‑1 (MQ=255)

                                                   tGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCgcgg                                                                                            <  1:561436/150‑1 (MQ=255)

                                                        attatattatTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAc                                                                                      >  1:598300/1‑151 (MQ=255)

                                                          tatattatTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAcgg                                                                                    >  2:591398/1‑151 (MQ=255)

                                                          tatattatTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAcg                                                                                     <  1:116282/150‑1 (MQ=255)

                                                           atattatTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAcggc                                                                                   <  2:291182/151‑1 (MQ=255)

                                                            tattatTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAcggcg                                                                                  >  2:343604/1‑151 (MQ=255)

                                                                atcgATAATGTCTCTATTCTCGACCTGCTCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAcggcgg                                                                                 >  2:239634/4‑148 (MQ=255)

                                                                         gTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGttt                                                                      <  2:486634/150‑1 (MQ=255)

                                                                             ctATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTAtt                                                                   >  2:388485/1‑149 (MQ=255)

                                                                                    cGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGGGCCAGGGGTTTTTTATGCTc                                                            >  1:100732/1‑149 (MQ=255)

                                                                                    cGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGATACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGGCCCAGGGTTTATTgctcct                                                             >  1:537069/1‑143 (MQ=255)

                                                                                        cTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTg                                                       >  2:511459/1‑150 (MQ=255)

                                                                                           aTCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCCGGGGTTATTTATGCTCACCCGc                                                      >  2:498320/1‑148 (MQ=255)

                                                                                             cAATAGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATAtt                                                 >  1:398463/1‑151 (MQ=255)

                                                                                                     gagaAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGCTTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAg                                         <  1:44125/151‑1 (MQ=255)

                                                                                                        aaaTTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCat                                      <  2:49594/151‑1 (MQ=255)

                                                                                                          aTTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTATTTATGTTCACCTGCATATTGAATCCAGCat                                      >  1:46774/1‑149 (MQ=255)

                                                                                                            ttCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatgat                                   <  2:468713/150‑1 (MQ=255)

                                                                                                            ttCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTATTGATGCTCACCTGCCTATTGAATCCAGCatgat                                   >  1:273443/1‑150 (MQ=255)

                                                                                                              ccGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCAtgatga                                 <  2:600372/150‑1 (MQ=255)

                                                                                                              ccGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCAtgatga                                 <  1:611889/150‑1 (MQ=255)

                                                                                                              ccGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGAc                                <  2:510373/151‑1 (MQ=255)

                                                                                                                   ccAATTGTGATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCgg                           <  2:49311/151‑1 (MQ=255)

                                                                                                                        tgtgATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACt                      >  2:27534/1‑151 (MQ=255)

                                                                                                                         gtgATTAAAGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACt                      <  1:384620/150‑1 (MQ=255)

                                                                                                                               aaaGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGaaa               <  1:590305/151‑1 (MQ=255)

                                                                                                                                aaGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAAc              <  1:416376/151‑1 (MQ=255)

                                                                                                                                 aGGACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAAcc             <  1:178920/151‑1 (MQ=255)

                                                                                                                                  ggACGTTACATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCg            <  1:639144/151‑1 (MQ=255)

                                                                                                                                         aCATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTAccc      >  2:543869/1‑150 (MQ=255)

                                                                                                                                         aCATTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCt     <  1:107311/151‑1 (MQ=255)

                                                                                                                                          cATTGCCGGCGTTGGCGCAGAATCCACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCGCCTGCATATTGAATCCAGCATGATGACGCCGGTCCCTTTTGAAACCGCTACCCt     <  1:78554/150‑1 (MQ=255)

                                                                                                                                           aTTGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGc   <  1:147393/151‑1 (MQ=255)

                                                                                                                                             tGCCGGCGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTCTTGATGCTCACCTGCATATTGAATCCAGCATGGTGACGCCGGTCACTTTTGAAACCGCTACCCTTcc  >  1:64177/1‑150 (MQ=255)

TAGCCGGGCTGAATACCAGGAATTGTTAGCCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCC  >  NC_000913/3843999‑3844289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A