Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F50 I2 R1 209 44.2 1360995 98.7% 1343302 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,381,504 T→C E272G (GAA→GGA)  frdA ← anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,381,5040TC100.0% 144.3 / NA 42E272G (GAA→GGA) frdAanaerobic fumarate reductase catalytic and NAD/flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/23);  total (19/23)

CCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGC  >  NC_000913/4381357‑4381647
                                                                                                                                                   |                                                                                                                                               
ccGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCg                                                                                                                                              <  1:97119/151‑1 (MQ=255)
 cGAGGTGACGCAAGTCGAGATAGACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCgg                                                                                                                                             >  2:427278/1‑151 (MQ=255)
  gAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCggg                                                                                                                                            >  2:465152/1‑151 (MQ=255)
   aGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGc                                                                                                                                           >  2:342616/1‑151 (MQ=255)
     gTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGccc                                                                                                                                         <  2:344241/151‑1 (MQ=255)
          gCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGcc                                                                                                                                    <  1:390254/151‑1 (MQ=255)
               tCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAAt                                                                                                                               >  2:587923/1‑151 (MQ=255)
                cGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAAt                                                                                                                               <  2:615096/150‑1 (MQ=255)
                      aaaCCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCtt                                                                                                                            >  1:61331/1‑147 (MQ=255)
                       aaCCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGc                                                                                                                          >  1:315738/1‑148 (MQ=255)
                        aCCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGc                                                                                                                          >  1:560884/1‑147 (MQ=255)
                                     gcggcgTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCAtt                                                                                                         <  2:678976/151‑1 (MQ=255)
                                     gcggcgTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCAtt                                                                                                         >  2:241698/1‑151 (MQ=255)
                                       ggcgTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCAtttt                                                                                                       <  2:49935/151‑1 (MQ=255)
                                            ggAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTttgttg                                                                                                  <  1:396433/151‑1 (MQ=255)
                                               gaTGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGAcc                                                                                               <  1:115917/151‑1 (MQ=255)
                                                 tGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGAcc                                                                                               <  2:244711/149‑1 (MQ=255)
                                                     gtTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAAt                                                                                          <  1:244340/150‑1 (MQ=255)
                                                        gCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCg                                                                                      >  1:426656/1‑151 (MQ=255)
                                                            ttACGCCATTCGTGGCATAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGATTTCCCGGGCCCAGGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGc                                                                                     <  2:32672/148‑1 (MQ=255)
                                                               cGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCAc                                                                               >  1:537742/1‑151 (MQ=255)
                                                                          ccAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCtt                                                                                  >  1:607193/1‑137 (MQ=255)
                                                                          ccAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCtt                                                                                  <  2:607175/137‑1 (MQ=255)
                                                                          ccAGAAGGCCGGAGAGCCTTTTTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCCCGCCCAGCGGATTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCa                                                                        <  2:304351/147‑1 (MQ=255)
                                                                           cAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCtt                                                                   <  1:18931/151‑1 (MQ=255)
                                                                              aaGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTc                                                                  <  2:672352/149‑1 (MQ=255)
                                                                                    tGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGtcatc                                                           >  1:368672/1‑150 (MQ=255)
                                                                                                           cAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCg                                   <  2:27564/151‑1 (MQ=255)
                                                                                                             gTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTTCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGt                                 >  2:433521/1‑151 (MQ=255)
                                                                                                                   tatatTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGt                            <  1:240624/150‑1 (MQ=255)
                                                                                                                    atatTTGTTTTGCGGCTCGCCCAGCGGTGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTg                           <  1:100605/150‑1 (MQ=255)
                                                                                                                     tatTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTTCAGATCACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGAt                         >  2:174229/1‑151 (MQ=255)
                                                                                                                     tatTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGa                          <  1:503425/150‑1 (MQ=255)
                                                                                                                           tttttCGGCTCGCCCAGGGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGa                    <  2:453103/150‑1 (MQ=255)
                                                                                                                           tttttCGGCCCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGa                    <  2:382932/150‑1 (MQ=255)
                                                                                                                            ttttGGGCTCGCCCAGCGCAGTTCCCAGGCCAATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACt                      <  2:29933/147‑1 (MQ=255)
                                                                                                                                cGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAAt              <  1:190273/151‑1 (MQ=255)
                                                                                                                                  gCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTc            >  1:562453/1‑151 (MQ=255)
                                                                                                                                      gCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATg        >  2:159068/1‑151 (MQ=255)
                                                                                                                                        ccAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTc      >  1:129847/1‑151 (MQ=255)
                                                                                                                                         cAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTc      >  2:621739/1‑150 (MQ=255)
                                                                                                                                            cGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGc  >  1:325240/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGC  >  NC_000913/4381357‑4381647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A