Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I3 R1 143 109.6 3434427 98.5% 3382910 150.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,381,596 A→G G241G (GGT→GGC)  frdA ← anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,381,5960AG98.0% 365.0 / ‑1.9 98G241G (GGT→GGC) frdAanaerobic fumarate reductase catalytic and NAD/flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (46/50);  total (48/50)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.85e-01

TTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCA  >  NC_000913/4381450‑4381740
                                                                                                                                                  |                                                                                                                                                
ttGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGcc                                                                                                                                                <  1:202409/149‑1 (MQ=255)
ttGTCGCG‑GGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGcca                                                                                                                                               <  1:702146/149‑2 (MQ=255)
   tCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCGGCGGAGTTTCCGGCCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGaa                                                                                                                                            <  1:339608/150‑1 (MQ=255)
   tCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAAc                                                                                                                                           >  2:1134912/1‑151 (MQ=255)
     gcgTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCt                                                                                                                                         >  1:843857/1‑151 (MQ=255)
       gTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCt                                                                                                                                         <  1:499967/149‑1 (MQ=255)
       gTGGACCCAGTTCCATATATTTGTTTTGCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTgg                                                                                                                                       <  2:258949/151‑1 (MQ=255)
        tGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGc                                                                                                                                      >  2:469924/1‑151 (MQ=255)
        tGGACCCAGTTCCATATATTTGTTTTACGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTTACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTgg                                                                                                                                       <  2:1484126/150‑1 (MQ=255)
         ggACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGc                                                                                                                                      <  2:1569877/150‑1 (MQ=255)
         ggACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGc                                                                                                                                      >  2:809167/1‑150 (MQ=255)
          gACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGc                                                                                                                                      >  1:666729/1‑149 (MQ=255)
            cccAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGAc                                                                                                                                  <  1:906936/151‑1 (MQ=255)
             ccAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGAcc                                                                                                                                 <  1:1180964/151‑1 (MQ=255)
             ccAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGAcc                                                                                                                                 >  2:1103235/1‑151 (MQ=255)
              cAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCg                                                                                                                                <  1:1682044/151‑1 (MQ=255)
                  tCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGtt                                                                                                                             <  2:579072/150‑1 (MQ=255)
                  tCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTg                                                                                                                            >  1:691545/1‑151 (MQ=255)
                  tCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTg                                                                                                                            >  2:642157/1‑151 (MQ=255)
                   ccATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTgg                                                                                                                           >  1:1452739/1‑151 (MQ=255)
                   ccATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTgg                                                                                                                           <  1:365568/151‑1 (MQ=255)
                        tatTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGAt                                                                                                                      >  1:587539/1‑151 (MQ=255)
                         atTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGAt                                                                                                                      <  2:370589/150‑1 (MQ=255)
                          tttGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATa                                                                                                                     <  1:835266/150‑1 (MQ=255)
                           ttGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATAc                                                                                                                    <  2:1067011/150‑1 (MQ=255)
                                tttCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAAc                                                                                                               <  1:1027339/150‑1 (MQ=255)
                                tttCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACg                                                                                                              >  2:341479/1‑151 (MQ=255)
                                   cGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAAt                                                                                                           <  1:388800/151‑1 (MQ=255)
                                    ggCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAAtt                                                                                                          <  1:218782/151‑1 (MQ=255)
                                         gCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATg                                                                                                     >  1:703061/1‑151 (MQ=255)
                                         gCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATg                                                                                                     >  1:1008103/1‑151 (MQ=255)
                                         gCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATg                                                                                                     >  2:1020054/1‑151 (MQ=255)
                                         gCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATg                                                                                                     >  2:601335/1‑151 (MQ=255)
                                           ccAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTc                                                                                                   >  2:925789/1‑151 (MQ=255)
                                           ccAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTc                                                                                                   <  2:1261711/151‑1 (MQ=255)
                                           ccAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGCAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATAAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTc                                                                                                   >  1:767526/1‑151 (MQ=255)
                                            cAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTc                                                                                                   >  1:629358/1‑150 (MQ=255)
                                                ggAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGc                                                                                               <  1:1496436/150‑1 (MQ=255)
                                                 gAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGATGGGTGATACTGAACGAATTCCATGTCACGc                                                                                               >  1:1084405/1‑149 (MQ=255)
                                                   gTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGc                                                                                            <  1:963971/150‑1 (MQ=255)
                                                   gTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCg                                                                                           >  2:1593933/1‑151 (MQ=255)
                                                      tCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGa                                                                                         >  1:1405163/1‑150 (MQ=255)
                                                       ccGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGaa                                                                                        <  1:873592/150‑1 (MQ=255)
                                                       ccGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGa                                                                                         <  2:234753/149‑1 (MQ=255)
                                                       ccGGCCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGa                                                                                         <  2:890754/149‑1 (MQ=255)
                                                        cGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACg                                                                                      <  2:976263/151‑1 (MQ=255)
                                                          ggCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGcc                                                                                    <  2:407718/151‑1 (MQ=255)
                                                           gCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCg                                                                                   >  2:888859/1‑151 (MQ=255)
                                                           gCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCg                                                                                   >  2:842364/1‑151 (MQ=255)
                                                              cATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTgg                                                                                >  1:970871/1‑151 (MQ=255)
                                                               aTGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGc                                                                               >  1:778503/1‑151 (MQ=255)
                                                               aTGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGc                                                                               >  2:159703/1‑151 (MQ=255)
                                                                tGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCt                                                                              >  2:739068/1‑151 (MQ=255)
                                                                 gCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCt                                                                              <  1:711331/150‑1 (MQ=255)
                                                                  ccGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCtt                                                                             >  1:176693/1‑150 (MQ=255)
                                                                           tGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGccat                                                                     <  1:1117910/149‑1 (MQ=255)
                                                                           tGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGcc                                                                       <  2:1066619/147‑1 (MQ=255)
                                                                            gCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGccatacc                                                                  <  2:581138/151‑1 (MQ=255)
                                                                             cAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGccataccc                                                                 <  2:850564/151‑1 (MQ=255)
                                                                                 tAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGccatacctatac                                                             >  1:771293/1‑151 (MQ=255)
                                                                                  aaCGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCcatacccatacc                                                            >  1:713789/1‑151 (MQ=255)
                                                                                    cGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGt                                                          <  1:1124174/151‑1 (MQ=255)
                                                                                      gTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCa                                                        <  2:136294/151‑1 (MQ=255)
                                                                                       tAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCAc                                                       >  1:1377556/1‑151 (MQ=255)
                                                                                        aGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCAcc                                                      <  1:339998/151‑1 (MQ=255)
                                                                                        aGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCAcc                                                      >  2:1070036/1‑151 (MQ=255)
                                                                                        aGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCAcc                                                      >  2:1611541/1‑151 (MQ=255)
                                                                                        aGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCAcc                                                      >  2:375339/1‑151 (MQ=255)
                                                                                          ccATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCgg                                                    <  1:1028648/151‑1 (MQ=255)
                                                                                           cATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGt                                                   <  2:1591286/151‑1 (MQ=255)
                                                                                           cATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGt                                                   <  1:1512088/151‑1 (MQ=255)
                                                                                           cATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGt                                                   >  1:1307848/1‑151 (MQ=255)
                                                                                               tttGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAc                                                <  2:176704/150‑1 (MQ=255)
                                                                                               tttGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAc                                                <  1:220251/150‑1 (MQ=255)
                                                                                               tttGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACg                                               >  2:1278434/1‑151 (MQ=255)
                                                                                                ttgttgACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACg                                               <  2:756149/150‑1 (MQ=255)
                                                                                                  gttgACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGAt                                             <  2:741699/150‑1 (MQ=255)
                                                                                                  gttgACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGAt                                             <  1:1567262/150‑1 (MQ=255)
                                                                                                      aCCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATgccgc                                        >  2:860063/1‑151 (MQ=255)
                                                                                                       ccAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATgccgcc                                       >  2:1259619/1‑151 (MQ=255)
                                                                                                         aGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGt                                     <  2:1452837/151‑1 (MQ=255)
                                                                                                          gAATCCCGCTTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGAAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATCCCCATACCGTCACCGGTAACGATGCCGCCGtt                                    <  2:666788/151‑1 (MQ=255)
                                                                                                              aCCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGgtg                                >  1:1336599/1‑151 (MQ=255)
                                                                                                               ccGCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGgtgt                               >  1:1706543/1‑151 (MQ=255)
                                                                                                                 gCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGtgttg                             >  2:850933/1‑151 (MQ=255)
                                                                                                                 gCCTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGtgttg                             >  1:790294/1‑151 (MQ=255)
                                                                                                                  ccTTCACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGtgttgt                            >  1:45187/1‑151 (MQ=255)
                                                                                                                      cACCGCGGCAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAAc                         <  1:127340/150‑1 (MQ=255)
                                                                                                                         cgcgGCAACCTTCGGTCATCATGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTCGGTTAGCGCCATACCCATACCGTCCCCGGTAACGATGCCGCCGTTGGTGTTGTAACGATa                     <  2:278956/151‑1 (MQ=255)
                                                                                                                            ggCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATaaa                   >  2:1151742/1‑150 (MQ=255)
                                                                                                                              cAACCTTCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAAcgc                >  2:1445948/1‑151 (MQ=255)
                                                                                                                                   ttCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGAccc           >  2:58868/1‑151 (MQ=255)
                                                                                                                                   ttCGGTCATCAGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGAccc           >  2:1610877/1‑151 (MQ=255)
                                                                                                                                         catcaGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCAcc      <  1:159695/150‑1 (MQ=255)
                                                                                                                                         catcaGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCAcc      <  1:375311/150‑1 (MQ=255)
                                                                                                                                          atcaGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGc    <  2:1147781/151‑1 (MQ=255)
                                                                                                                                            caGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCa  <  1:1134824/151‑1 (MQ=255)
                                                                                                                                             aGGATGCCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGcc   >  2:1325821/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCA  >  NC_000913/4381450‑4381740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A