Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F50 I2 R1 104 44.1 1376607 98.9% 1361464 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,382,351 C→T intergenic (‑33/‑292) frdA ← / → epmA anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit/Elongation Factor P Lys34 lysyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,382,3510CT100.0% 212.9 / NA 57intergenic (‑33/‑292)frdA/epmAanaerobic fumarate reductase catalytic and NAD/flavoprotein subunit/Elongation Factor P Lys34 lysyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/31);  total (26/31)

TTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTAAATTATC  >  NC_000913/4382205‑4382495
                                                                                                                                                  |                                                                                                                                                
ttttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGt                                                                                                                                              >  1:340714/1‑151 (MQ=255)
  ttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGTGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGACTGTTTTTATCACACAGCTAAGTAc                                                                                                                                            >  1:87439/1‑151 (MQ=255)
  ttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTa                                                                                                                                             <  2:635569/150‑1 (MQ=255)
  ttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTAc                                                                                                                                            >  2:25527/1‑151 (MQ=255)
            tGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCCGATTGTTTTTATCCCACCGCTACGTACTTCAGGGTa                                                                                                                                   >  1:488435/1‑150 (MQ=255)
            tGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTa                                                                                                                                   >  2:317667/1‑150 (MQ=255)
                  ttttGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTAcc                                                                                                                             <  1:415257/150‑1 (MQ=255)
                    ttGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTg                                                                                                                           <  1:556400/150‑1 (MQ=255)
                    tagCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTg                                                                                                                           >  2:156495/3‑150 (MQ=255)
                     tGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGa                                                                                                                          >  1:412914/1‑150 (MQ=255)
                      gCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGaa                                                                                                                         >  1:618123/1‑150 (MQ=255)
                       cATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTg                                                                                                                           >  2:382872/1‑147 (MQ=255)
                       cATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAg                                                                                                                       <  1:555732/151‑1 (MQ=255)
                        aTTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGt                                                                                                                      <  1:83698/151‑1 (MQ=255)
                         ttCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGtt                                                                                                                     >  2:610340/1‑151 (MQ=255)
                          tCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGtt                                                                                                                     >  2:70188/1‑150 (MQ=255)
                           cGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTa                                                                                                                    <  1:262460/150‑1 (MQ=255)
                           cGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTAc                                                                                                                   <  2:453186/151‑1 (MQ=255)
                            ggATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACg                                                                                                                  <  1:300331/151‑1 (MQ=255)
                                  gCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGc                                                                                                            <  2:340725/151‑1 (MQ=255)
                                      gcgcGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGaa                                                                                                         <  1:154887/150‑1 (MQ=255)
                                      gcgcGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAAc                                                                                                        <  1:625182/151‑1 (MQ=255)
                                        gcgGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGc                                                                                                      <  2:679421/151‑1 (MQ=255)
                                          ggCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTa                                                                                                    <  2:613866/151‑1 (MQ=255)
                                            cagcaATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGCTCGGCTTGAAAGGTCTGCCCGACATTCCTCCAGATTGTTTTTATCCCACAGCTACTTACTTCAGGGTAAGTACCTCAAAGTTACGGTCTGCGAATGCTAtt                                                                                                  >  1:344121/1‑151 (MQ=255)
                                            cagcaATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTAtt                                                                                                  >  1:532948/1‑151 (MQ=255)
                                                aaTTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCAc                                                                                              >  1:248673/1‑151 (MQ=255)
                                                                   gcgcCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTCTTCCACTGCTCCTTTATAGGTaca                                                                            >  1:624442/1‑150 (MQ=255)
                                                                   gcgcCACCGGCGCATACAATGTCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTGCGTACTTCAGGGTAAGTGCCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGGaca                                                                            <  1:457468/150‑1 (MQ=255)
                                                                     gcCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTacaaca                                                                         <  1:113253/151‑1 (MQ=255)
                                                                       cACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGt                                                                       <  1:376427/151‑1 (MQ=255)
                                                                        aCCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGt                                                                       >  2:271121/1‑150 (MQ=255)
                                                                          cGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGGTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGtat                                                                     >  2:511567/1‑150 (MQ=255)
                                                                           ggCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAg                                                                   <  1:573248/151‑1 (MQ=255)
                                                                             cgcCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGt                                                                  >  1:217161/1‑150 (MQ=255)
                                                                              gcCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCt                                                                <  1:181821/151‑1 (MQ=255)
                                                                                  aCAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATg                                                            <  2:672550/151‑1 (MQ=255)
                                                                                       ggCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATaa                                                       <  1:14652/151‑1 (MQ=255)
                                                                                          aaGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTc                                                    >  2:108094/1‑151 (MQ=255)
                                                                                           aGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCt                                                   <  1:3144/151‑1 (MQ=255)
                                                                                                 gCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAAtt                                             <  1:383459/151‑1 (MQ=255)
                                                                                                 gCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAAtt                                             >  1:42753/1‑151 (MQ=255)
                                                                                                     gAATGGTTTGATCGGAATTCCTCCAGATTGTTTTTATCCCACAGCTGCGTTCGTCAGGGTAAGTGCCTGAAAGTTACGGTCTGCGAACGCTATGCCACTGCTCCTGTATAGGTACACCAGGATAGTCTGATGGATAAGTCTGAAATATGa                                          <  1:544846/150‑1 (MQ=255)
                                                                                                       aaGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACga                                       <  1:528917/151‑1 (MQ=255)
                                                                                                            ttGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACg                                        >  1:578868/1‑145 (MQ=255)
                                                                                                            ttGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATc                                   <  1:60933/150‑1 (MQ=255)
                                                                                                              gCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGa                                 <  1:72798/150‑1 (MQ=255)
                                                                                                                aCGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGAttt                              <  2:336091/151‑1 (MQ=255)
                                                                                                                   aCATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGAtttttt                           >  2:156653/1‑151 (MQ=255)
                                                                                                                    cATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGAttttttt                          >  2:437233/1‑151 (MQ=255)
                                                                                                                        cctccAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTg                      <  1:228003/151‑1 (MQ=255)
                                                                                                                         ctccAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTgc                     >  2:180499/1‑151 (MQ=255)
                                                                                                                                  gTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAggg            <  2:621588/151‑1 (MQ=255)
                                                                                                                                  gTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAggg            >  2:568523/1‑151 (MQ=255)
                                                                                                                                  gTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAgg             <  1:465838/150‑1 (MQ=255)
                                                                                                                                          cccACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTaaagt     <  1:564936/150‑3 (MQ=255)
                                                                                                                                             acaGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTAAATTATc  >  1:667523/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTAAATTATC  >  NC_000913/4382205‑4382495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A