Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F50 I3 R1 105 107.9 3491495 95.1% 3320411 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,982,684 (C)8→10 noncoding (79/87 nt) leuT → tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,982,6761.C98.1% 374.2 / ‑0.9 105noncoding (71/87 nt)leuTtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (46/57);  total (47/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
*NC_0009133,982,6762.C92.4% 357.4 / 23.4 105noncoding (71/87 nt)leuTtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (5/3);  new base C (42/55);  total (47/58)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.50e-01

Only 100 of 123 total aligned reads displayed.

TGGTAGAGCCCTGGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTG  >  NC_000913/3982526‑3982817
                                                                                                                                                         ||                                                                                                                                                
tGGTAGAGCCCTGGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑t                                                                                                                                                    >  1:241459/1‑151 (MQ=255)
 ggTAGAGCGCTGGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACACCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑t                                                                                                                                                    <  1:536850/150‑1 (MQ=255)
      agCCCTGGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGTGTTCAAG‑‑T‑‑‑ccccc‑‑c                                                                                                                                         <  4:212960/151‑1 (MQ=255)
       gCCCTGGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑‑ccccc‑‑cc                                                                                                                                        <  4:272439/151‑1 (MQ=255)
        cccTGGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑‑ccccc‑‑ccc                                                                                                                                       <  4:290483/151‑1 (MQ=255)
        cccTGGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑‑ccccc‑‑ccc                                                                                                                                       >  2:1099120/1‑151 (MQ=255)
         ccTGGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑‑ccccc‑‑cccc                                                                                                                                      >  1:803421/1‑150 (MQ=255)
         ccTGGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑‑ccccc‑‑cccc                                                                                                                                      >  1:905960/1‑150 (MQ=255)
          cTGGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑‑ccccc‑‑cccct                                                                                                                                     >  3:365237/1‑149 (MQ=255)
           tGGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑‑ccccc‑‑ccccc                                                                                                                                     >  1:899752/1‑148 (MQ=255)
            ggATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑‑ccccc‑‑ccccct                                                                                                                                    <  1:248766/150‑4 (MQ=255)
            ggATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑‑ccccc‑‑ccccc                                                                                                                                     >  1:572483/1‑147 (MQ=255)
             gATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCCCCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑‑ccccc‑‑ccccctcg                                                                                                                                  <  1:716224/151‑6 (MQ=255)
             gATTGTGATTCCAGGTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑‑ccccc‑‑ccccctc                                                                                                                                   <  3:24154/150‑5 (MQ=255)
              aTTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑‑ccccc‑‑ccccctcg                                                                                                                                  >  2:165883/1‑145 (MQ=255)
                 gtgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑‑CCCCC‑‑CCCTCGcacc                                                                                                                                >  4:402131/1‑150 (MQ=255)
                    aTTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACg                                                                                                                             <  1:1136682/151‑1 (MQ=255)
                       ccAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCCCGACt                                                                                                                          >  1:924548/1‑151 (MQ=255)
                        cAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACtt                                                                                                                         <  2:1212109/151‑1 (MQ=255)
                         aGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACttt                                                                                                                        <  3:301872/151‑1 (MQ=255)
                          gTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTa                                                                                                                       <  2:770833/151‑1 (MQ=255)
                               cGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGa                                                                                                                   >  1:1093107/1‑150 (MQ=255)
                                  gggTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTg                                                                                                               <  2:1170345/151‑1 (MQ=255)
                                   ggTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGa                                                                                                              <  4:391066/151‑1 (MQ=255)
                                    gTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGa                                                                                                              <  1:1029836/150‑1 (MQ=255)
                                     ttCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGaa                                                                                                             >  2:752736/1‑150 (MQ=255)
                                      tCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAAc                                                                                                            <  3:306584/150‑1 (MQ=255)
                                      tCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACt                                                                                                           >  2:759107/1‑151 (MQ=255)
                                       cGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACt                                                                                                           >  4:318536/1‑150 (MQ=255)
                                       cGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACt                                                                                                           >  4:164212/1‑150 (MQ=255)
                                         aaTCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTaa                                                                                                         >  4:124838/1‑150 (MQ=255)
                                         aaTCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTaaa                                                                                                        >  4:439530/1‑150 (MQ=255)
                                          aTCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTaaaa                                                                                                       <  3:201688/151‑1 (MQ=255)
                                          aTCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTaaaa                                                                                                       <  2:553576/151‑1 (MQ=255)
                                          aTCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTaaa                                                                                                        >  3:251318/1‑150 (MQ=255)
                                            cccATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGGCC‑‑‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAt                                                                                                     <  2:458600/151‑1 (MQ=255)
                                            cccATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGAGGCGGAATTGGTAGACGAGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑‑CCCCCCTCCCTCGCACCACCACTTTAAAGAATTGAACTaaaa                                                                                                       >  1:107159/1‑149 (MQ=21)
                                              cATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAtt                                                                                                    >  1:235263/1‑150 (MQ=255)
                                                 tAGCCCCCCCATTTTTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTTGTAGACGCGCCTGCTTCAGGTTTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCCCCCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCa                                                                                                  <  2:228342/150‑1 (MQ=21)
                                                  aGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaa                                                                                                <  2:1274775/150‑1 (MQ=255)
                                                   gCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                                                                              <  2:796296/151‑1 (MQ=255)
                                                   gCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                                                                              <  1:965144/151‑1 (MQ=255)
                                                     cACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGGAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                                                                              <  2:672483/149‑1 (MQ=255)
                                                        cccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGt                                                                                         <  1:1280433/151‑1 (MQ=255)
                                                        cccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGt                                                                                         >  4:123117/1‑151 (MQ=255)
                                                         ccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGt                                                                                         >  2:28266/1‑150 (MQ=255)
                                                         ccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGt                                                                                         <  1:28271/150‑1 (MQ=255)
                                                         ccATTATTAGAAGTTGTGACAATGCGAAGGGGGCGGAATTTGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAg                                                                                          <  1:818015/149‑1 (MQ=25)
                                                           attattAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAtt                                                                                      <  2:1092803/151‑1 (MQ=255)
                                                            ttattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAATGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAtt                                                                                      <  2:8613/150‑1 (MQ=255)
                                                             tattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAttt                                                                                     <  2:662101/150‑1 (MQ=255)
                                                              attaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                                                                    <  2:753201/150‑1 (MQ=255)
                                                              attaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                                                                    <  2:376107/150‑1 (MQ=255)
                                                              attaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCg                                                                                   <  1:994642/151‑1 (MQ=255)
                                                               ttaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCgg                                                                                  >  2:526888/1‑151 (MQ=255)
                                                                taGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCgg                                                                                  <  2:637566/150‑1 (MQ=255)
                                                                taGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCgg                                                                                  <  3:57546/150‑1 (MQ=255)
                                                                taGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGc                                                                                 >  2:1122187/1‑151 (MQ=255)
                                                                 aGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCg                                                                                >  1:1280967/1‑151 (MQ=255)
                                                                       tgtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGGGt                                                                             >  1:7379/1‑148 (MQ=255)
                                                                        gtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcg                                                                         >  1:397179/1‑151 (MQ=255)
                                                                        gtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgc                                                                          <  1:11617/150‑1 (MQ=255)
                                                                         tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcgc                                                                        >  4:287376/1‑151 (MQ=255)
                                                                            cAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCt                                                                    >  1:979566/1‑151 (MQ=255)
                                                                              aTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCtt                                                                   <  4:370982/151‑1 (MQ=255)
                                                                               tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTg                                                                  >  1:73245/1‑151 (MQ=255)
                                                                                gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGa                                                                                                              <  4:130937/106‑1 (MQ=255)
                                                                                gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGa                                                                                                              >  3:130936/1‑106 (MQ=255)
                                                                                  gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCCCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGt                                                                >  3:259673/1‑150 (MQ=255)
                                                                                   aaGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGt                                                                >  4:11409/1‑149 (MQ=255)
                                                                                       tGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAgcgc                                                           >  2:278378/1‑150 (MQ=255)
                                                                                         gCGGAATTGGTAGACGCGCTAGCTTCAGGGGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTACCACCACGACTTTAAAAAATAAAACTAAAAAATAAAAAAGCAGGATTTCGGCGAGTATCGCAGCTTTGTaaggca                                                          >  2:1186858/1‑144 (MQ=17)
                                                                                           ggAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTg                                                      <  3:154359/151‑1 (MQ=255)
                                                                                             aaTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGt                                                    >  2:621409/1‑151 (MQ=255)
                                                                                                tGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGttt                                                  <  2:195909/150‑1 (MQ=255)
                                                                                                      aCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGAcc                                            >  2:346075/1‑150 (MQ=255)
                                                                                                          gcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTgg                                       >  2:300146/1‑151 (MQ=255)
                                                                                                            tAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGt                                     >  2:672811/1‑151 (MQ=255)
                                                                                                              gCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCg                                   >  2:861712/1‑151 (MQ=255)
                                                                                                                 tCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGGGGGTCgg                                  >  2:371297/1‑149 (MQ=255)
                                                                                                                   aGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGt                              <  2:69364/151‑1 (MQ=255)
                                                                                                                     gtgtTAGTGTCCTTACGGACGTTGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAACAAGCAGTATTTCGGCGAAGAGCGCACCTTGGTAGGGAAACGGGATTGGGCCCAGTGGGACGGTGGTTc                            >  2:830693/1‑151 (MQ=25)
                                                                                                                       gtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCg                           <  4:343378/150‑1 (MQ=255)
                                                                                                                       gtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGa                          <  1:930833/151‑1 (MQ=255)
                                                                                                                       gtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCg                           >  1:480552/1‑149 (MQ=255)
                                                                                                                         tAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGaa                         <  2:1006704/150‑1 (MQ=255)
                                                                                                                         tAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAAt                        >  1:74057/1‑151 (MQ=255)
                                                                                                                          aGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAAt                        <  2:230258/150‑1 (MQ=255)
                                                                                                                             gtCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCctc                    >  3:175551/1‑151 (MQ=255)
                                                                                                                             gtCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCctc                    >  4:34502/1‑151 (MQ=255)
                                                                                                                              tCCTTACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCct                     <  1:1044423/149‑1 (MQ=255)
                                                                                                                                  tACGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCctctct                 <  2:1226568/149‑1 (MQ=255)
                                                                                                                                    cGGACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGcc             >  3:157219/1‑151 (MQ=255)
                                                                                                                                      gACGTGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGa           <  2:572372/151‑1 (MQ=255)
                                                                                                                                          tGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGGGTCCTCTCTCGCCGACCa        >  2:5876/1‑150 (MQ=255)
                                                                                                                                          tGGGGGTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCa        >  3:378600/1‑150 (MQ=255)
                                                                                                                                            ggggTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAAtt     <  1:1191513/151‑1 (MQ=255)
                                                                                                                                              ggTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAAtttt   <  1:216060/151‑1 (MQ=255)
                                                                                                                                              ggTTCAAG‑‑TC‑‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTg  <  2:89903/151‑1 (MQ=255)
                                                                                                                                               gTTCAAG‑‑TCC‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTg  >  4:56066/1‑151 (MQ=255)
                                                                                                                                                         ||                                                                                                                                                
TGGTAGAGCCCTGGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑‑CCCCC‑‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTG  >  NC_000913/3982526‑3982817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A