Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I2 R1	150	68.2	2132745	98.7%	2105019	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,192,212	A→G	D140D (GAT→GAC)	hflD ←	putative lysogenization regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,192,212	0	A	G	100.0%	197.3 / NA	52	D140D (GAT→GAC)	hflD	putative lysogenization regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/30); total (22/30)

GACGTCCGCCGCCGACCTGGTGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCAC  >  NC_000913/1192067‑1192352

gACGTCCGCCGCCGACCTGGTGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAAc                                                                                                                                         >  1:408041/1‑151 (MQ=255)

  cGTCCGCCGCCGACCTGGTGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACat                                                                                                                                       <  1:311839/151‑1 (MQ=255)

      cgccgccgACCTGGTGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACat                                                                                                                                       >  1:536445/1‑147 (MQ=255)

       gccgccgACCTGGTGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACatagata                                                                                                                                  <  1:827243/151‑1 (MQ=255)

          gccgACCTGGTGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATagca                                                                                                                               <  1:344392/151‑1 (MQ=255)

           ccgACCTGGTGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATagcag                                                                                                                              <  1:14416/151‑1 (MQ=255)

            cgACCTGGTGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATagcagc                                                                                                                             >  1:381412/1‑151 (MQ=255)

               ccTGGTGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCAt                                                                                                                          >  2:84618/1‑151 (MQ=255)

                  ggTGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTTACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATcg                                                                                                                        <  2:509397/150‑1 (MQ=255)

                      ccAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCtc                                                                                                                   <  2:852545/151‑1 (MQ=255)

                      ccAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCtc                                                                                                                   >  1:963432/1‑151 (MQ=255)

                        agagCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCtcat                                                                                                                 <  1:144178/151‑1 (MQ=255)

                        agagCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCtcat                                                                                                                 >  1:424672/1‑151 (MQ=255)

                           gCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAg                                                                                                              <  1:848653/151‑1 (MQ=255)

                              cggcggCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAGCATAGATAGCAGCCATCGCGCTCATCAGc                                                                                                             <  2:259222/149‑1 (MQ=255)

                              cggcggCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGt                                                                                                           >  2:492953/1‑151 (MQ=255)

                                   gcgcGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCgg                                                                                                      <  1:579/151‑1 (MQ=255)

                                      cgAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACt                                                                                                   <  2:968282/151‑1 (MQ=255)

                                       gAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTg                                                                                                  >  1:450784/1‑151 (MQ=255)

                                         aTGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTa                                                                                                <  1:346921/151‑1 (MQ=255)

                                           gcctgccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTaa                                                                                               <  2:275591/150‑1 (MQ=255)

                                            cctgccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAAt                                                                                             >  1:398952/1‑151 (MQ=255)

                                            cctgccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAAt                                                                                             <  2:807087/151‑1 (MQ=255)

                                             ctgccAGCAGGGATGCGGGAACTTTCTCCTGCACGTGCGGGCTTTGCAGTACAGCAAGGGAACCTGTGCCCTGAATGCGCGGGCCAAACGGGCTAATAACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGAATGTAAATc                                                                                            >  2:520663/1‑151 (MQ=255)

                                                   gcagGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAgtg                                                                                      <  1:269634/151‑1 (MQ=255)

                                                           gcgcGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCt                                                                              <  1:16940/151‑1 (MQ=255)

                                                            cgcgAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTg                                                                             >  1:753310/1‑151 (MQ=255)

                                                            cgcgAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTg                                                                             <  1:422921/151‑1 (MQ=255)

                                                                 aCTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGtt                                                                        <  2:152773/151‑1 (MQ=255)

                                                                     tCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCCCGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCCAAGTGTTCGAGCTGGCGTTGCa                                                                     <  2:194021/150‑1 (MQ=255)

                                                                            cACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTg                                                             >  2:654534/1‑151 (MQ=255)

                                                                             aCTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTg                                                             >  2:693625/1‑150 (MQ=255)

                                                                             aCTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGa                                                            <  1:692233/151‑1 (MQ=255)

                                                                             aCTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGa                                                            <  1:791825/151‑1 (MQ=255)

                                                                                          gCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCa                                                <  1:831364/150‑1 (MQ=255)

                                                                                           cAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCaga                                              <  1:352912/151‑1 (MQ=255)

                                                                                            aGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCag                                               >  2:54872/1‑149 (MQ=255)

                                                                                               aCAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAgtgt                                          >  1:810420/1‑151 (MQ=255)

                                                                                                      gggAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAgcgc                                   <  2:155988/151‑1 (MQ=255)

                                                                                                            cGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAGCGCGCCttt                             <  2:363731/151‑1 (MQ=255)

                                                                                                                gACCTGAATGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAGCGCGCCTTTCGCt                         >  2:418992/1‑151 (MQ=255)

                                                                                                                      aaTGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAGCGCGCCTTTCGCTgaggag                   <  1:419003/151‑1 (MQ=255)

                                                                                                                       aTGCGCGGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAGCGCGCCTTTCGCTgagg                     >  2:152405/1‑148 (MQ=255)

                                                                                                                            cgGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAGCGCGCCTTTCGCTGAGGAGAGTTTg             <  1:605562/151‑1 (MQ=255)

                                                                                                                            cgGGCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAGCGCGCCTTTCGCTGAGGAGAGTTTg             <  2:923506/151‑1 (MQ=255)

                                                                                                                               gCCAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAGCGCGCCTTTCGCTGAGGAGAGTTTgcgc          <  2:587170/151‑1 (MQ=255)

                                                                                                                                ccAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCt         >  2:676181/1‑151 (MQ=255)

                                                                                                                                ccAAGCGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCt         <  2:1037260/151‑1 (MQ=255)

                                                                                                                                     cGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGc    >  2:395351/1‑151 (MQ=255)

                                                                                                                                     cGGGCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGc    >  2:313987/1‑151 (MQ=255)

                                                                                                                                      gggCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGc    >  2:995817/1‑150 (MQ=255)

                                                                                                                                       ggCTAATCACGTCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCAc  >  1:915419/1‑151 (MQ=255)

GACGTCCGCCGCCGACCTGGTGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGAGCTGGCGTTGCAGGCCGTTGATCCGGTTGCCCAGAGTGTCGAGCGCGCCTTTCGCTGAGGAGAGTTTGCGCTCAAGCAC  >  NC_000913/1192067‑1192352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A