Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,116,797	A→G	intergenic (‑215/‑10)	lpxL ← / → yceA	lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase/putative rhodanese‑related sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,116,797	0	A	G	100.0%	159.2 / NA	45	intergenic (‑215/‑10)	lpxL/yceA	lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase/putative rhodanese‑related sulfurtransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/23); total (22/23)

AAATTTGTGGCTGAAGACTGGGCGAAATTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGC  >  NC_000913/1116649‑1116937

aaaTTTGTGGCTGAAGACTGGGCGAAATTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTGAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGcc                                                                                                                                            <  1:219944/151‑1 (MQ=255)

                aCTGGGCGAAATTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAgtg                                                                                                                            <  1:144088/151‑1 (MQ=255)

                aCTGGGCGAAATTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAgtg                                                                                                                            <  2:292586/151‑1 (MQ=255)

                   gggCGAAATTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTa                                                                                                                         <  2:652113/151‑1 (MQ=255)

                        aaaTTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACaa                                                                                                                    <  1:607932/151‑1 (MQ=255)

                          aTTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAAcc                                                                                                                  <  2:403154/151‑1 (MQ=255)

                                     tGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCaa                                                                                                        <  1:283893/150‑1 (MQ=255)

                                     tGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAac                                                                                                       >  2:558951/1‑151 (MQ=255)

                                                 ataatTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTaaa                                                                                            >  1:6513/1‑150 (MQ=255)

                                                   aatTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGc                                                                                         >  1:383024/1‑151 (MQ=255)

                                                      ttttAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCa                                                                                       <  2:166633/150‑1 (MQ=255)

                                                       tttAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCaa                                                                                      <  2:571261/150‑1 (MQ=255)

                                                        ttAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCaaa                                                                                     >  2:502033/1‑150 (MQ=255)

                                                         tAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAAt                                                                                   >  1:576366/1‑151 (MQ=255)

                                                             gcgcAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGtt                                                                                <  1:206248/150‑1 (MQ=255)

                                                              cgcAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTgg                                                                              >  1:77231/1‑151 (MQ=255)

                                                                cAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCt                                                                            <  1:455256/151‑1 (MQ=255)

                                                                   aTGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTgag                                                                         <  2:303587/151‑1 (MQ=255)

                                                                   aTGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTgag                                                                         <  2:376845/151‑1 (MQ=255)

                                                                       aGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcg                                                                     >  1:66490/1‑151 (MQ=255)

                                                                        gCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcga                                                                    <  2:40264/151‑1 (MQ=255)

                                                                           tAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcgaacc                                                                 >  1:288283/1‑151 (MQ=255)

                                                                            aaaaTGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcgaacc                                                                 <  2:564249/150‑1 (MQ=255)

                                                                               aTGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcgaaccgcga                                                             <  2:185379/151‑1 (MQ=255)

                                                                               aTGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcgaaccgcg                                                              >  1:496369/1‑150 (MQ=255)

                                                                                         gTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCAttt                                                   >  2:561920/1‑151 (MQ=255)

                                                                                         gTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCAttt                                                   >  1:222780/1‑151 (MQ=255)

                                                                                           tAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCg                                                 >  1:144006/1‑151 (MQ=255)

                                                                                             aTTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGtt                                               <  2:302801/151‑1 (MQ=255)

                                                                                                    aTAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACa                                          >  2:423209/1‑149 (MQ=255)

                                                                                                     tAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGt                                       >  2:143366/1‑151 (MQ=255)

                                                                                                             atatCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTcc                                 <  2:438294/149‑1 (MQ=255)

                                                                                                               atCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACAt                             >  2:451481/1‑151 (MQ=255)

                                                                                                                 cATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATc                            <  2:352/150‑1 (MQ=255)

                                                                                                                 cATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATc                            >  1:644577/1‑150 (MQ=255)

                                                                                                                  aTGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGc                          >  1:560617/1‑151 (MQ=255)

                                                                                                                         ggATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCt                   >  1:367340/1‑151 (MQ=255)

                                                                                                                          gATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCt                   >  1:196842/1‑150 (MQ=255)

                                                                                                                           aTTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTaa                 <  2:460071/151‑1 (MQ=255)

                                                                                                                              tttACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAgg               <  2:49129/150‑1 (MQ=255)

                                                                                                                                  cTTTCCCATCTCGCAGGAGCCGTACACCATGTCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGAcc          >  2:417342/1‑151 (MQ=255)

                                                                                                                                         aTCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACg   >  1:86382/1‑151 (MQ=255)

                                                                                                                                          tctcGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACg   >  1:104980/1‑150 (MQ=255)

                                                                                                                                             cGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACg   <  1:155993/147‑1 (MQ=255)

                                                                                                                                             cGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGc  <  2:499296/148‑1 (MQ=255)

AAATTTGTGGCTGAAGACTGGGCGAAATTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGC  >  NC_000913/1116649‑1116937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A