Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 193 39.9 1341687 91.8% 1231668 149.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,129,249 T→C L471L (TTG→CTG)  murJ → putative lipid II flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,129,2490TC100.0% 103.8 / NA 29L471L (TTG→CTG) murJputative lipid II flippase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/14);  total (15/14)

GCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGT  >  NC_000913/1129102‑1129395
                                                                                                                                                   |                                                                                                                                                    
gCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTgg                                                                                                                                                   <  1:271356/151‑1 (MQ=255)
       tgctTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCAt                                                                                                                                            >  2:614823/1‑151 (MQ=255)
        gctTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATg                                                                                                                                           >  2:116057/1‑151 (MQ=255)
         ctTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATg                                                                                                                                           >  1:523713/1‑150 (MQ=255)
          tttACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGcc                                                                                                                                         >  2:557468/1‑151 (MQ=255)
               tGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGc                                                                                                                                    >  2:180382/1‑151 (MQ=255)
                ggCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCg                                                                                                                                   <  2:251227/151‑1 (MQ=255)
                   aGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGttt                                                                                                                                >  1:23389/1‑151 (MQ=255)
                         gTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCg                                                                                                                          >  1:210759/1‑151 (MQ=255)
                         gTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCg                                                                                                                          <  1:81826/151‑1 (MQ=255)
                            aGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGttt                                                                                                                       <  2:189350/151‑1 (MQ=255)
                              cAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTa                                                                                                                      >  1:604302/1‑150 (MQ=255)
                               aGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAAt                                                                                                                    >  1:419498/1‑151 (MQ=255)
                                gAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATg                                                                                                                   >  2:276825/1‑151 (MQ=255)
                                  aaaTCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGc                                                                                                                 >  1:406477/1‑151 (MQ=255)
                                         tACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGGGAGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCgggcctg                                                                                                          >  1:304945/1‑146 (MQ=255)
                                              cGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGc                                                                                                     <  2:220385/151‑1 (MQ=255)
                                                      ggCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTg                                                                                            <  2:276696/151‑1 (MQ=255)
                                                         tGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑ggggggt                                                                                             >  2:335626/1‑144 (MQ=255)
                                                            aTGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑ggggg                                                                                               >  2:594347/1‑141 (MQ=255)
                                                                          gCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTg                                                                       <  2:604308/151‑1 (MQ=255)
                                                                                        cGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCtt                                                         <  1:645704/151‑1 (MQ=255)
                                                                                            aCTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCaa                                                      <  2:650345/150‑1 (MQ=255)
                                                                                                            gTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGccc                                     <  2:101322/151‑1 (MQ=255)
                                                                                                                 tttAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCccgcc                                  <  1:335623/149‑1 (MQ=255)
                                                                                                                         tGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGGGCTGGCGGGGGGTTGCC‑CGGTCTTCCCCGCCCCTGGGGGGACGGGGGTTCAAAATTAAAAAATTTTCCCCCCggggggg                           >  2:306786/1‑144 (MQ=255)
                                                                                                                          gTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTaa                       <  2:253914/151‑1 (MQ=255)
                                                                                                                                 atCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGc                 <  1:228467/150‑1 (MQ=255)
                                                                                                                                               gTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagt  <  1:585548/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGT  >  NC_000913/1129102‑1129395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A