Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,684,690	G→A	A273V (GCG→GTG)	glyA ←	serine hydroxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,684,690	0	G	A	97.4%	130.4 / NA	38	A273V (GCG→GTG)	glyA	serine hydroxymethyltransferase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (24/13); total (25/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAGTGGTGG  >  NC_000913/2684548‑2684834

cagGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCg                                                                                                                                          >  1:472722/1‑151 (MQ=255)

             ttATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGc                                                                                                                              >  1:285010/1‑150 (MQ=255)

              tATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCAt                                                                                                                            >  2:574578/1‑151 (MQ=255)

                       cgccgGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGAcc                                                                                                                   >  1:207091/1‑151 (MQ=255)

                        gccgGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCg                                                                                                                  <  2:508463/151‑1 (MQ=255)

                            gAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGAt                                                                                                                                     <  2:375929/128‑1 (MQ=255)

                            gAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGAt                                                                                                                                     >  1:375925/1‑128 (MQ=255)

                            gAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCt                                                                                                              <  2:605405/151‑1 (MQ=255)

                                     ttGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGAccacca                                                                                                      <  1:562483/150‑1 (MQ=255)

                                             gcgcTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACg                                                                                             >  1:226022/1‑151 (MQ=255)

                                                cTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGc                                                                                           >  2:288988/1‑150 (MQ=255)

                                                cTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGc                                                                                           >  1:481391/1‑150 (MQ=255)

                                                   gAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCag                                                                                         >  1:108317/1‑149 (MQ=255)

                                                     ggAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGtt                                                                                     >  1:352921/1‑151 (MQ=255)

                                                       aaCACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCa                                                                                   <  1:34794/151‑1 (MQ=255)

                                                           cTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGCAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGttt                                                                               >  1:178364/1‑151 (MQ=255)

                                                            ttCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGtttt                                                                              >  2:224765/1‑151 (MQ=255)

                                                                   aTCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTAc                                                                        >  1:351632/1‑150 (MQ=255)

                                                                     cGCTTTAGCGTTTCTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGc                                                                     <  2:529914/151‑1 (MQ=255)

                                                                          tAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCtt                                                                 <  2:317431/150‑1 (MQ=255)

                                                                                   tAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTacca                                                         >  1:643845/1‑149 (MQ=255)

                                                                                    aGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTaccacc                                                       <  2:285009/150‑1 (MQ=255)

                                                                                       gACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTc                                                   >  1:82424/1‑151 (MQ=255)

                                                                                         ccTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGc                                                 <  1:144365/151‑1 (MQ=255)

                                                                                          ctgctgctgGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGcc                                                >  1:474497/1‑151 (MQ=255)

                                                                                              tgctgGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAgg                                             >  1:548503/1‑150 (MQ=255)

                                                                                              tgctgGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAgg                                             >  2:489787/1‑150 (MQ=255)

                                                                                                  ggTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATc                                          >  2:531417/1‑149 (MQ=255)

                                                                                                   gTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAgg                                       <  2:352924/151‑1 (MQ=255)

                                                                                                     gagttgggAACTCAGGCTCCAGGGCAACGTGCAGAGCAAACGCTTTACCGGCGCTGGCGTGCGTCAACGGACAAAAAAAACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGgcc                                     >  1:53897/8‑151 (MQ=255)

                                                                                                              aCTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGGTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCgg                            >  2:252701/1‑151 (MQ=255)

                                                                                                                    gCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGc                      >  1:611927/1‑151 (MQ=255)

                                                                                                                            gCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGttt              >  2:122551/1‑151 (MQ=255)

                                                                                                                              ttcttTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGtttt             >  2:448891/1‑150 (MQ=255)

                                                                                                                                     agagCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAgtgg     >  1:346164/1‑151 (MQ=255)

                                                                                                                                     agagCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAgtg      <  2:589875/150‑1 (MQ=255)

                                                                                                                                         cAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAGtggtgg  <  1:505795/150‑1 (MQ=255)

                                                                                                                                          aaCCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGCCCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAGtggtgg  <  1:574573/149‑1 (MQ=255)

CAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAGTGGTGG  >  NC_000913/2684548‑2684834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A