Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,698,706	C→A	intergenic (‑156/‑53)	pgpC ← / → yfhH	phosphatidylglycerophosphatase C, membrane bound/putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,698,706	0	C	A	100.0%	157.1 / NA	43	intergenic (‑156/‑53)	pgpC/yfhH	phosphatidylglycerophosphatase C, membrane bound/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (25/18); total (25/18)

CTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGC  >  NC_000913/2698567‑2698853

cTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGtctc                                                                                                                                          >  1:141905/1‑151 (MQ=255)

      cATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTg                                                                                                                                    >  1:198307/1‑151 (MQ=255)

         ccTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCt                                                                                                                                 >  1:660009/1‑151 (MQ=255)

            gATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCtgtt                                                                                                                              >  2:336900/1‑151 (MQ=255)

                aaTTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTc                                                                                                                           <  2:204881/150‑1 (MQ=255)

                aaTTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCg                                                                                                                          >  1:58953/1‑151 (MQ=255)

                 aTTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGc                                                                                                                         <  2:51302/151‑1 (MQ=255)

                 aTTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGc                                                                                                                         >  2:392526/1‑151 (MQ=255)

                   tttCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGcc                                                                                                                        <  2:323456/150‑1 (MQ=255)

                            tacATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCAt                                                                                                              >  2:69056/1‑151 (MQ=255)

                                     tAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGa                                                                                                      >  1:358585/1‑150 (MQ=255)

                                      aaCAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGaaa                                                                                                    <  2:111808/151‑1 (MQ=255)

                                             agatCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATg                                                                                              <  1:450091/150‑1 (MQ=255)

                                                    caCTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCtt                                                                                      <  2:198308/151‑1 (MQ=255)

                                                         ttCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACtt                                                                                  <  1:651703/150‑1 (MQ=255)

                                                               caacaaTCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATcc                                                                           <  1:77278/151‑1 (MQ=255)

                                                                      cGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCg                                                                    >  2:126040/1‑151 (MQ=255)

                                                                              tcatGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAgg                                                            >  2:62816/1‑151 (MQ=255)

                                                                                   gCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCtt                                                       <  1:576793/151‑1 (MQ=255)

                                                                                     tAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGc                                                     >  1:501856/1‑151 (MQ=255)

                                                                                       aCTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGccc                                                   <  2:488861/151‑1 (MQ=255)

                                                                                        cTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGccc                                                   >  1:238074/1‑150 (MQ=255)

                                                                                          ggAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGccc                                                   >  2:45909/1‑148 (MQ=255)

                                                                                          ggAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCaga                                                <  1:251438/151‑1 (MQ=255)

                                                                                           gAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATACTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCag                                                 >  1:111886/1‑149 (MQ=255)

                                                                                              tttttAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTg                                             >  2:426345/1‑150 (MQ=255)

                                                                                                tttAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAt                                           >  2:151377/1‑150 (MQ=255)

                                                                                                 ttAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATa                                          >  1:503/1‑150 (MQ=255)

                                                                                                  tAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATaa                                         <  2:548188/150‑1 (MQ=255)

                                                                                                  tAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATaa                                         <  1:217124/150‑1 (MQ=255)

                                                                                                         tGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTgg                                 >  1:45273/1‑151 (MQ=255)

                                                                                                              aTTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGAt                            >  1:51257/1‑151 (MQ=255)

                                                                                                                 aGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACTAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTa                          <  1:573438/150‑1 (MQ=255)

                                                                                                                 aGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTa                          <  1:17384/150‑1 (MQ=255)

                                                                                                                   cGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATct                       <  1:150432/151‑1 (MQ=255)

                                                                                                                       aaTGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATctgctg                   >  1:293938/1‑151 (MQ=255)

                                                                                                                         tGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTgctgct                 >  1:551255/1‑151 (MQ=255)

                                                                                                                          ggAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTCACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTgctgct                 >  1:493556/1‑150 (MQ=255)

                                                                                                                           gAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTAc               >  2:156735/1‑151 (MQ=255)

                                                                                                                                    aTTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATa      <  1:372420/151‑1 (MQ=255)

                                                                                                                                     ttCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCGGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATAc     >  1:379763/1‑151 (MQ=255)

                                                                                                                                       cACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACgg   >  1:479577/1‑151 (MQ=255)

                                                                                                                                        aCTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGgc  <  1:284376/151‑1 (MQ=255)

CTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGC  >  NC_000913/2698567‑2698853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A