Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,048,315	C→T	V243I (GTC→ATC)	gcvP ←	glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,048,315	0	C	T	100.0%	152.2 / NA	41	V243I (GTC→ATC)	gcvP	glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/19); total (22/19)

AAAGAATGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCA  >  NC_000913/3048181‑3048460

aaaGAATGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGAt                                                                                                                                   <  2:264174/151‑1 (MQ=255)

aaaGAATGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGAt                                                                                                                                   >  2:109566/1‑151 (MQ=255)

 aaGAATGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGAtt                                                                                                                                  >  1:89550/1‑151 (MQ=255)

     aTGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTc                                                                                                                                >  2:166297/1‑149 (MQ=255)

     aTGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTc                                                                                                                                >  1:509868/1‑149 (MQ=255)

     aTGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTc                                                                                                                                >  2:490672/1‑149 (MQ=255)

       gCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGtt                                                                                                                            <  1:58911/151‑1 (MQ=255)

            gtgtGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCta                                                                                                                       <  2:528730/151‑1 (MQ=255)

             tgtgGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCta                                                                                                                       >  1:158298/1‑150 (MQ=255)

                 ggCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAg                                                                                                                  <  2:426068/151‑1 (MQ=255)

                          aGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAgtgt                                                                                                          >  2:80171/1‑150 (MQ=255)

                            cccATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGt                                                                                                       <  1:580804/151‑1 (MQ=255)

                             ccATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTc                                                                                                      <  1:608976/151‑1 (MQ=255)

                                  ggCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGaa                                                                                                 <  1:273086/151‑1 (MQ=255)

                                      cGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTc                                                                                             >  1:3003/1‑151 (MQ=255)

                                            aGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGt                                                                                       <  1:379707/151‑1 (MQ=255)

                                                        aaCCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACctg                                                                           <  1:250690/151‑1 (MQ=255)

                                                         aCCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACctgc                                                                          <  2:339377/151‑1 (MQ=255)

                                                           cAAAAACAATATGCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACctgctg                                                                        >  1:224213/1‑151 (MQ=255)

                                                              aaaCAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTa                                                                      >  2:331672/1‑150 (MQ=255)

                                                                            cccTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGa                                                          >  1:371513/1‑148 (MQ=255)

                                                                              cTGTTTACCCTGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCCGATCACAGTGTTGCGTGATTGCAGTTCGCTAATACGGGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGa                                                       >  1:59831/1‑149 (MQ=255)

                                                                                   tACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCt                                                 <  2:269967/150‑1 (MQ=255)

                                                                                   tACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTg                                                >  2:472230/1‑151 (MQ=255)

                                                                                   tACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTg                                                >  2:175313/1‑151 (MQ=255)

                                                                                     cccGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGAt                                              >  2:175154/1‑151 (MQ=255)

                                                                                          cgcAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCg                                         <  2:645553/151‑1 (MQ=255)

                                                                                           gcAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCg                                         >  1:115900/1‑150 (MQ=255)

                                                                                              gTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCa                                     <  1:175312/151‑1 (MQ=255)

                                                                                                  aCAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACttt                                 >  1:660130/1‑151 (MQ=255)

                                                                                                   cAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACtttt                                <  1:112815/151‑1 (MQ=255)

                                                                                                     gCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTg                              >  1:57163/1‑151 (MQ=255)

                                                                                                      cACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTgc                             <  1:72701/151‑1 (MQ=255)

                                                                                                        ccAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTgcgc                           <  2:154822/151‑1 (MQ=255)

                                                                                                        ccAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTgcgc                           <  2:592127/151‑1 (MQ=255)

                                                                                                          aGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGcgcg                          >  1:75818/1‑150 (MQ=255)

                                                                                                               cATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCg                    >  1:470992/1‑151 (MQ=255)

                                                                                                                     atCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGGAGTCGTGAATTTCACCAGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATc              >  1:385021/1‑151 (MQ=255)

                                                                                                                              aaCGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCaaa      >  1:64532/1‑150 (MQ=255)

                                                                                                                                 gCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCa  <  2:307642/151‑1 (MQ=255)

                                                                                                                                 gCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCa  <  2:624547/151‑1 (MQ=255)

AAAGAATGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCA  >  NC_000913/3048181‑3048460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A