Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,086,618	A→G	intergenic (+216/‑88)	yqgC → / → metK	uncharacterized protein/S‑adenosylmethionine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,086,618	0	A	G	100.0%	203.5 / NA	55	intergenic (+216/‑88)	yqgC/metK	uncharacterized protein/S‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (33/22); total (33/22)

AGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCT  >  NC_000913/3086477‑3086765

aGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGc                                                                                                                                               >  1:13098/1‑148 (MQ=255)

   aaaaaTTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTc                                                                                                                                         >  1:322194/1‑151 (MQ=255)

    aaaaTTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCg                                                                                                                                        <  1:81856/151‑1 (MQ=255)

      aaTTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTg                                                                                                                                      >  1:46701/1‑151 (MQ=255)

       aTTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTG‑TTTTGACCTGCAGAGTCGTGGt                                                                                                                                    <  2:326712/151‑1 (MQ=255)

            cAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAgg                                                                                                                                 >  2:343255/1‑150 (MQ=255)

                       ttAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACcac                                                                                                                     >  1:236471/1‑151 (MQ=255)

                        tAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACcac                                                                                                                     <  2:159792/150‑1 (MQ=255)

                         aaCAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACcaca                                                                                                                    <  1:373937/150‑1 (MQ=255)

                           cAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGa                                                                                                                  >  1:395555/1‑150 (MQ=255)

                              aaTGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAAt                                                                                                              >  1:380781/1‑151 (MQ=255)

                              aaTGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAAt                                                                                                              <  1:441014/151‑1 (MQ=255)

                                  agaCACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCcac                                                                                                          <  2:578418/151‑1 (MQ=255)

                                      acGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCcacaca                                                                                                       >  1:631701/1‑150 (MQ=255)

                                            tcaaaaaaaGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGttt                                                                                                >  1:331021/3‑151 (MQ=255)

                                            cAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGttt                                                                                                >  2:12230/1‑151 (MQ=255)

                                              aaaaaaaGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTg                                                                                               >  2:658123/1‑150 (MQ=255)

                                                aaaaaGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGc                                                                                            <  2:172395/151‑1 (MQ=255)

                                                  aaaGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTa                                                                                          <  1:437621/151‑1 (MQ=255)

                                                      tGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAAcc                                                                                       >  1:658585/1‑150 (MQ=255)

                                                        gAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCaa                                                                                     >  1:576285/1‑150 (MQ=255)

                                                           aTAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATtc                                                                                 >  1:277662/1‑151 (MQ=255)

                                                             aGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATtctc                                                                               <  1:537544/151‑1 (MQ=255)

                                                             aGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATtctc                                                                               <  1:394129/151‑1 (MQ=255)

                                                              gggTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTctct                                                                              >  2:650304/1‑151 (MQ=255)

                                                               ggTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCtt                                                                             <  1:126940/151‑1 (MQ=255)

                                                                   aagaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAgg                                                                         <  1:67822/151‑1 (MQ=255)

                                                                    agaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGt                                                                        <  2:536837/151‑1 (MQ=255)

                                                                     gaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTg                                                                       >  2:460674/1‑151 (MQ=255)

                                                                        ttGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGat                                                                     >  2:518252/1‑150 (MQ=255)

                                                                        ttGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGat                                                                     >  1:581374/1‑150 (MQ=255)

                                                                               aaaaTAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTaaa                                                               <  2:485706/149‑1 (MQ=255)

                                                                                  aTAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATgg                                                          <  2:194780/151‑1 (MQ=255)

                                                                                       cATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCaaa                                                      >  2:383340/1‑150 (MQ=255)

                                                                                        aTCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAac                                                    >  2:255300/1‑151 (MQ=255)

                                                                                         accAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGATTTTGACGTGCAGAGTCGTGGTAGGATCCGCTAAGACAGAACATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCaaaa                                                     >  1:662329/2‑149 (MQ=255)

                                                                                          ccAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAacac                                                  >  1:456686/1‑151 (MQ=255)

                                                                                            aGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCt                                                <  1:615348/151‑1 (MQ=255)

                                                                                               tGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCtttt                                             >  2:411501/1‑151 (MQ=255)

                                                                                                gTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCtttt                                             <  1:336804/150‑1 (MQ=255)

                                                                                                      ccatccatACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTcc                                      >  1:265913/1‑151 (MQ=255)

                                                                                                       catccatACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCg                                     >  2:427326/1‑151 (MQ=255)

                                                                                                       catccatACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCg                                     <  2:145225/151‑1 (MQ=255)

                                                                                                            atACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTcc                                <  2:442748/151‑1 (MQ=255)

                                                                                                              aCCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCg                               <  2:236472/150‑1 (MQ=255)

                                                                                                              aCCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGt                              <  2:631711/151‑1 (MQ=255)

                                                                                                                 gATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGtctc                           >  2:500114/1‑151 (MQ=255)

                                                                                                                     aaCACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGa                        >  1:514892/1‑150 (MQ=255)

                                                                                                                       cacTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAgg                     <  1:518249/151‑1 (MQ=255)

                                                                                                                          tCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGc                   >  2:524285/1‑150 (MQ=255)

                                                                                                                            aGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATc                >  1:119073/1‑151 (MQ=255)

                                                                                                                             gACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATcc               >  1:659756/1‑151 (MQ=255)

                                                                                                                                 gCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGAc           >  2:543250/1‑151 (MQ=255)

                                                                                                                                     gtgtTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACaaa        >  2:39775/1‑150 (MQ=255)

                                                                                                                                          tttGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCt  >  1:641650/1‑151 (MQ=255)

AGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCT  >  NC_000913/3086477‑3086765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A