Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,449,100	T→C	T24A (ACG→GCG)	rplP ←	50S ribosomal subunit protein L16

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,449,100	0	T	C	100.0%	137.3 / NA	38	T24A (ACG→GCG)	rplP	50S ribosomal subunit protein L16
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/14); total (24/14)

GTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGTACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAAC  >  NC_000913/3448956‑3449244

gTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTg                                                                                                                                            <  1:127745/151‑1 (MQ=255)

          gAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACcg                                                                                                                                  <  2:436328/151‑1 (MQ=255)

                       cAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCtt                                                                                                                     >  1:247346/1‑151 (MQ=255)

                           tCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTtgtg                                                                                                                 >  2:359550/1‑151 (MQ=255)

                           tCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTtgtg                                                                                                                 >  1:649620/1‑151 (MQ=255)

                              tACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTtgt                                                                                                                  <  2:296683/147‑1 (MQ=255)

                              tACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCa                                                                                                               >  1:371016/1‑150 (MQ=255)

                                     aCGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACgg                                                                                                       <  2:85496/151‑1 (MQ=255)

                                       gCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGa                                                                                                      >  2:144310/1‑150 (MQ=255)

                                       gCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGa                                                                                                      >  1:578065/1‑150 (MQ=255)

                                        cTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAAt                                                                                                    >  1:614457/1‑151 (MQ=255)

                                          tAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAAt                                                                                                    <  2:523885/149‑1 (MQ=255)

                                                      tCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTgg                                                                                      >  1:654966/1‑151 (MQ=255)

                                                       cATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTgg                                                                                      <  1:4330/150‑1 (MQ=255)

                                                        aTAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGtt                                                                                    >  2:337817/1‑151 (MQ=255)

                                                        aTAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGt                                                                                     <  2:249581/150‑1 (MQ=255)

                                                           gCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGt                                                                                  <  1:104852/150‑1 (MQ=255)

                                                             acgacgTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAAc                                                                               <  2:479329/151‑1 (MQ=255)

                                                                 cgTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATc                                                                            >  2:37726/1‑150 (MQ=255)

                                                                  gTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATc                                                                            >  2:632251/1‑149 (MQ=255)

                                                                        ctTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAAGTTTGTACGCTTTGGTTGTAACATCAGCGACg                                                                     >  1:52560/1‑150 (MQ=255)

                                                                                 gACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCTCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTAttt                                                           <  1:571843/151‑1 (MQ=255)

                                                                                 gACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTa                                                              <  1:318810/148‑1 (MQ=255)

                                                                                 gACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTAt                                                             <  1:592985/149‑1 (MQ=255)

                                                                                                   cACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACgctgctg                                         <  2:667008/151‑1 (MQ=255)

                                                                                                         cAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCttttt                                   >  1:233211/1‑151 (MQ=255)

                                                                                                          aaCAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCttttt                                   >  1:358130/1‑150 (MQ=255)

                                                                                                                   cAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGagc                          >  2:245652/1‑150 (MQ=255)

                                                                                                                    aGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGagc                          >  1:70441/1‑149 (MQ=255)

                                                                                                                         gAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTACTTTTTAGGCTGAGCAGCCGGt                   >  1:596698/1‑151 (MQ=255)

                                                                                                                           aGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGttt                 >  2:542451/1‑151 (MQ=255)

                                                                                                                            gCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGtttt                <  1:144306/151‑1 (MQ=255)

                                                                                                                            gCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGtttt                >  1:284447/1‑151 (MQ=255)

                                                                                                                                ccGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCg            >  2:115566/1‑151 (MQ=255)

                                                                                                                                 cGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCgg           >  1:569374/1‑151 (MQ=255)

                                                                                                                                        aaCATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTc     >  1:452128/1‑150 (MQ=255)

                                                                                                                                         aCATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCa    >  1:231496/1‑150 (MQ=255)

                                                                                                                                            tCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAAc  >  1:357901/1‑149 (MQ=255)

GTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGTACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAAC  >  NC_000913/3448956‑3449244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A