Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,511,488	A→G	L286P (CTC→CCC)	yhfZ ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,511,488	0	A	G	100.0%	97.9 / NA	29	L286P (CTC→CCC)	yhfZ	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/11); total (18/11)

ACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAG  >  NC_000913/3511340‑3511630

aCGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATgggcgggc                                                                                                                                              <  1:393967/151‑1 (MQ=255)

             gCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTa                                                                                                                                  <  2:668851/150‑1 (MQ=255)

                        ttGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGc                                                                                                                       >  2:95037/1‑150 (MQ=255)

                                gTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACgcagc                                                                                                                >  2:322039/1‑149 (MQ=255)

                                      aTCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGttgttg                                                                                                        <  2:125414/151‑1 (MQ=255)

                                       tCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGc                                                                                                       >  1:581754/1‑151 (MQ=255)

                                          aTAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCAtt                                                                                                    <  2:607601/151‑1 (MQ=255)

                                             aCGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTggg                                                                                                 <  2:329791/151‑1 (MQ=255)

                                               gcctgcctCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTGTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTa                                                                                               <  2:581759/151‑1 (MQ=255)

                                                  tgcctCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAatc                                                                                            >  1:218495/1‑151 (MQ=255)

                                                  tgcctCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAAATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAatc                                                                                            >  2:654352/1‑151 (MQ=255)

                                                         cAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACt                                                                                     >  1:279337/1‑151 (MQ=255)

                                                         cAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACt                                                                                     >  2:123862/1‑151 (MQ=255)

                                                               aGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTc                                                                               >  2:613619/1‑151 (MQ=255)

                                                                  ttGAGACTGGTTTCCATATCAGATGACTTTAGTTTAATTACTTGGAAGAGGATGCGTACTCAATACCCGAGGGAGATGGGCGGGCAGGGCGTGTGTAGCTACAACGGCACGCAGCAGTTGTTGCATTGGGAAATCATCGACTCGCGTCAg                                                                             >  2:63986/1‑150 (MQ=255)

                                                                        cTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACg                                                                      >  2:393811/1‑151 (MQ=255)

                                                                           gTTTCCATATCCGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCt                                                                   >  1:479275/1‑151 (MQ=255)

                                                                                   atCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTgg                                                            <  2:131563/150‑1 (MQ=255)

                                                                                   atCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGc                                                           >  2:491747/1‑151 (MQ=255)

                                                                                    tCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGcc                                                          >  1:537041/1‑151 (MQ=255)

                                                                                     cAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGcc                                                          <  2:360353/150‑1 (MQ=255)

                                                                                                 gTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATcgcg                                             >  2:50100/1‑151 (MQ=255)

                                                                                                       taaCTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGt                                       >  1:403686/1‑151 (MQ=255)

                                                                                                        aaCTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTc                                      >  2:194430/1‑151 (MQ=255)

                                                                                                            cGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCtgt                                   <  2:256469/150‑1 (MQ=255)

                                                                                                                  cTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAgcgg                             >  1:233475/1‑150 (MQ=255)

                                                                                                                      tCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCg                        >  1:459965/1‑151 (MQ=255)

                                                                                                                              cactACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTaa                 <  1:440601/150‑1 (MQ=255)

                                                                                                                                     cGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCa         <  2:479737/151‑1 (MQ=255)

                                                                                                                                            gATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAg  <  2:448876/151‑1 (MQ=255)

ACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAG  >  NC_000913/3511340‑3511630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A