Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,525,382	A→G	T838A (ACG→GCG)	mrcA →	penicillin‑binding protein 1a, murein transglycosylase and transpeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,525,382	0	A	G	100.0%	126.4 / NA	36	T838A (ACG→GCG)	mrcA	penicillin‑binding protein 1a, murein transglycosylase and transpeptidase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/21); total (15/21)

GCCGGAGCAGCCGCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTC  >  NC_000913/3525234‑3525527

gCCGGAGCAGCCGCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCg                                                                                                                                                 >  2:54925/1‑151 (MQ=255)

   ggAGCAGCCGCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGAcc                                                                                                                                              >  2:62223/1‑151 (MQ=255)

     agcagcCGCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCAt                                                                                                                                            <  2:647060/151‑1 (MQ=255)

              tGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATa                                                                                                                                    >  2:423557/1‑150 (MQ=255)

                aCGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATg                                                                                                                                 <  1:261294/151‑1 (MQ=255)

                aCGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATg                                                                                                                                 >  1:274464/1‑151 (MQ=255)

                             gTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGa                                                                                                                    <  2:274464/151‑1 (MQ=255)

                               aTTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGaa                                                                                                                   <  2:69596/150‑1 (MQ=255)

                                  gtgACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTg                                                                                                                <  2:296247/150‑1 (MQ=255)

                                     aCGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTc                                                                                                             <  2:521282/150‑1 (MQ=255)

                                     aCGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCt                                                                                                            <  2:327354/151‑1 (MQ=255)

                                       ggTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTg                                                                                                           >  1:279731/1‑150 (MQ=255)

                                            atatCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTaaa                                                                                                     <  1:323703/151‑1 (MQ=255)

                                                cgatcgCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTACACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGGTCTGATTAAAAAgg                                                                                                 >  1:161770/1‑151 (MQ=255)

                                                  atcgCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGCACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGgcg                                                                                               >  2:491860/1‑151 (MQ=255)

                                                      cagcaCCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTc                                                                                           >  2:45936/1‑151 (MQ=255)

                                                                  gTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCa                                                                               <  2:362570/151‑1 (MQ=255)

                                                                   ttAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTc                                                                                >  1:364596/1‑149 (MQ=255)

                                                                    tAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAg                                                                              <  1:387227/150‑1 (MQ=255)

                                                                    tAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAg                                                                              <  2:445353/150‑1 (MQ=255)

                                                                            ggtggCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGAc                                                                     <  2:79468/151‑1 (MQ=255)

                                                                            ggtggCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGAc                                                                     <  1:342302/151‑1 (MQ=255)

                                                                                    cAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGc                                                              >  2:353490/1‑150 (MQ=255)

                                                                                                     tCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGAt                                             >  1:521166/1‑150 (MQ=255)

                                                                                                         cGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGc                                        <  1:301349/151‑1 (MQ=255)

                                                                                                                  gCAGCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAAcgcg                               >  1:277555/1‑151 (MQ=255)

                                                                                                                     gCCGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTa                            <  2:482849/151‑1 (MQ=255)

                                                                                                                      ccGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTa                            <  1:353487/150‑1 (MQ=255)

                                                                                                                       cGACACAACAGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATc                          <  2:213976/151‑1 (MQ=255)

                                                                                                                             aacaGGCAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCt                     >  2:661857/1‑150 (MQ=255)

                                                                                                                                   cAGTGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACaaaa              <  1:339847/151‑1 (MQ=255)

                                                                                                                                      tGCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCg           >  2:252308/1‑151 (MQ=255)

                                                                                                                                       gCACGAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGt          <  2:265094/151‑1 (MQ=255)

                                                                                                                                           gAGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGaa       >  1:325864/1‑150 (MQ=255)

                                                                                                                                            aGGTGGGAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGaaaa     <  2:293208/151‑1 (MQ=255)

                                                                                                                                                gggAGCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTc  <  2:279730/150‑1 (MQ=255)

GCCGGAGCAGCCGCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTC  >  NC_000913/3525234‑3525527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A