Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,612,540	T→C	W226R (TGG→CGG)	yhhT →	UPF0118 family putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,612,540	0	T	C	100.0%	102.6 / NA	30	W226R (TGG→CGG)	yhhT	UPF0118 family putative transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/14); total (16/14)

GAATAATCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGTGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATGGT  >  NC_000913/3612392‑3612685

gAATAATCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCgg                                                                                                                                                 >  2:550889/1‑151 (MQ=255)

    aatCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCa                                                                                                                                              <  2:385299/150‑1 (MQ=255)

        cacaGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACt                                                                                                                                         <  1:350675/151‑1 (MQ=255)

        cacaGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACt                                                                                                                                         >  1:453432/1‑151 (MQ=255)

                  caTCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTg                                                                                                                               >  1:54921/1‑151 (MQ=255)

                    tCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCt                                                                                                                             >  2:300647/1‑151 (MQ=255)

                            ttACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACg                                                                                                                     >  2:290411/1‑151 (MQ=255)

                                           aaaGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGgcg                                                                                                      >  1:656710/1‑151 (MQ=255)

                                                cGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTa                                                                                                 <  1:524912/151‑1 (MQ=255)

                                                 gTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTa                                                                                                 >  1:141484/1‑150 (MQ=255)

                                                         cTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTccgc                                                                                         >  1:644748/1‑150 (MQ=255)

                                                           aTCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTccgcc                                                                                        <  2:483923/149‑1 (MQ=255)

                                                              ttGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTAcc                                                                                   >  2:55593/1‑151 (MQ=255)

                                                                     gAAGACGCTACTCAGTTTATGGACAGGTGCCATCTTCTGGCGGGGGCTGGAGTTGATGGGGGTGCAGTTTGCGTTGATGCGGGCAGTACTGGCTTTTTGGCTCAACTACGTGCCCAATACCGGCGGGGTAATTTCCGCCGTACCGCCAAt                                                                             <  1:400496/150‑1 (MQ=255)

                                                                             tACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGt                                                                    >  1:494366/1‑151 (MQ=255)

                                                                                 cAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGtgct                                                                 <  1:580338/150‑1 (MQ=255)

                                                                                 cAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGtgct                                                                 <  2:44918/150‑1 (MQ=255)

                                                                                  aGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGtgctgc                                                               >  2:64565/1‑151 (MQ=255)

                                                                                       aTGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGttt                                                          <  1:605645/151‑1 (MQ=255)

                                                                                       aTGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGATTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGttt                                                          >  1:350940/1‑151 (MQ=255)

                                                                                                 gtCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGttt                                                <  1:32422/151‑1 (MQ=255)

                                                                                                   cATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTAc                                              <  2:181191/151‑1 (MQ=255)

                                                                                                       gTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAAt                                          >  1:51455/1‑151 (MQ=255)

                                                                                                             ctggggctggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTc                                    >  2:592503/1‑151 (MQ=255)

                                                                                                                    tggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCgg                             >  2:630829/1‑151 (MQ=255)

                                                                                                                           gATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTg                      <  1:577777/151‑1 (MQ=255)

                                                                                                                              gggggTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGtt                    <  1:630819/150‑1 (MQ=255)

                                                                                                                                   tGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGt              >  2:606976/1‑151 (MQ=255)

                                                                                                                                            cgcTGATGCGGGCAGTACTGGCGTTGTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCata      <  2:133708/150‑1 (MQ=255)

                                                                                                                                                gATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATGGt  <  2:430579/150‑1 (MQ=255)

GAATAATCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGTGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATGGT  >  NC_000913/3612392‑3612685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A