Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,770,892	T→C	D163G (GAC→GGC)	yibH ←	putative membrane fusion protein (MFP) component of efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,770,892	0	T	C	100.0%	149.8 / NA	42	D163G (GAC→GGC)	yibH	putative membrane fusion protein (MFP) component of efflux pump
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/26); total (16/26)

TCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTAT  >  NC_000913/3770745‑3771036

tCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCAc                                                                                                                                               >  1:668237/1‑151 (MQ=255)

tCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCAc                                                                                                                                               >  1:644203/1‑151 (MQ=255)

 cAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACg                                                                                                                                              <  1:360379/151‑1 (MQ=255)

     aaGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTcc                                                                                                                                          <  1:178215/151‑1 (MQ=255)

     aaGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTcc                                                                                                                                          <  2:427658/151‑1 (MQ=255)

     aaGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTc                                                                                                                                           <  2:384521/150‑1 (MQ=255)

      aGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCg                                                                                                                                         >  1:218574/1‑151 (MQ=255)

            gcTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACg                                                                                                                                   >  1:379084/1‑151 (MQ=255)

                ttAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGAtt                                                                                                                               >  1:264924/1‑151 (MQ=255)

                    gCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCAcc                                                                                                                           >  2:90505/1‑151 (MQ=255)

                    gCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCAcc                                                                                                                           <  2:218575/151‑1 (MQ=255)

                       cacaATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCAccgcc                                                                                                                        <  2:454357/151‑1 (MQ=255)

                        acaATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCAccgcc                                                                                                                        >  1:8916/1‑150 (MQ=255)

                                   ttGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCt                                                                                                            >  2:145984/1‑151 (MQ=255)

                                    tGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCt                                                                                                            <  1:119028/150‑1 (MQ=255)

                                           cGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCCCCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTa                                                                                                    <  1:529219/151‑1 (MQ=255)

                                           cGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTa                                                                                                    <  2:294786/151‑1 (MQ=255)

                                              tAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAAc                                                                                                  <  2:462041/150‑1 (MQ=255)

                                                           gAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCATCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTa                                                                                    <  1:247059/151‑1 (MQ=255)

                                                                 gCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGa                                                                              <  2:7041/151‑1 (MQ=255)

                                                                      ggATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACgg                                                                           <  1:92742/149‑1 (MQ=255)

                                                                      ggATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACg                                                                            <  2:509850/148‑1 (MQ=255)

                                                                            gcgcCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTcgc                                                                       <  1:408567/147‑1 (MQ=255)

                                                                            gcgcCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTcgc                                                                       <  2:369292/147‑1 (MQ=255)

                                                                            gcgcCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTc                                                                   <  1:18287/151‑1 (MQ=255)

                                                                                         cACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTgg                                                      <  2:331572/151‑1 (MQ=255)

                                                                                              aGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGggtggt                                                 >  2:337986/1‑151 (MQ=255)

                                                                                                ccTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGgtgt                                               >  2:503642/1‑151 (MQ=255)

                                                                                                       ccAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTg                                        <  1:51685/151‑1 (MQ=255)

                                                                                                          gcgcATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTgcgc                                     >  1:19750/1‑151 (MQ=255)

                                                                                                             cATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTc                                  <  1:340409/151‑1 (MQ=255)

                                                                                                              aTCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTc                                  >  2:362465/1‑150 (MQ=255)

                                                                                                              aTCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCa                                 <  1:574630/151‑1 (MQ=255)

                                                                                                              aTCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCa                                 <  1:488739/151‑1 (MQ=255)

                                                                                                                ccTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGt                               >  2:108354/1‑151 (MQ=255)

                                                                                                                      cgAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTg                         <  2:248037/151‑1 (MQ=255)

                                                                                                                       gAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTgc                        >  2:520136/1‑151 (MQ=255)

                                                                                                                             aTTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGcgcacgc                  <  1:556281/151‑1 (MQ=255)

                                                                                                                                 tGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCg              >  2:137583/1‑151 (MQ=255)

                                                                                                                                   ccGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTc            >  1:344496/1‑151 (MQ=255)

                                                                                                                                         aTCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTtata      <  1:411750/151‑1 (MQ=255)

                                                                                                                                              cgATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTAtattat  <  2:665791/150‑1 (MQ=255)

TCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTAT  >  NC_000913/3770745‑3771036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A