Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,788,944	T→C	F282L (TTT→CTT)	yibQ →	putative polysaccharide deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,788,944	0	T	C	100.0%	142.7 / NA	37	F282L (TTT→CTT)	yibQ	putative polysaccharide deacetylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/19); total (18/19)

GCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGA  >  NC_000913/3788811‑3789088

gCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGc                                                                                                                                    <  1:194668/148‑1 (MQ=255)

gCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGa                                                                                                                                  <  2:652923/150‑1 (MQ=255)

    aatgaaCCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCa                                                                                                                             <  1:197001/151‑1 (MQ=255)

     atgaaCCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCAt                                                                                                                            >  1:318974/1‑151 (MQ=255)

       gaaCCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATc                                                                                                                           <  1:309634/150‑1 (MQ=255)

              aGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCag                                                                                                                     >  1:458416/1‑149 (MQ=255)

                    aTACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCt                                                                                                             <  2:125502/151‑1 (MQ=255)

                      aCTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGa                                                                                                           <  2:350233/151‑1 (MQ=255)

                               ccgccgAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACt                                                                                                  >  1:104687/1‑151 (MQ=255)

                                cgccgAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACtt                                                                                                 >  1:575509/1‑151 (MQ=255)

                                 gccgAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTc                                                                                                >  2:338590/1‑151 (MQ=255)

                                  ccgAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTcc                                                                                               >  1:213953/1‑151 (MQ=255)

                                        aaCGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAACAt                                                                                          <  1:282664/150‑1 (MQ=255)

                                        aaCGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAACAt                                                                                          >  2:282663/1‑150 (MQ=255)

                                              gtgCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTg                                                                                     <  1:520590/149‑1 (MQ=255)

                                                               tAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAAc                                                                    >  2:322621/1‑149 (MQ=255)

                                                                aaTCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGc                                                                 <  2:380056/151‑1 (MQ=255)

                                                                     gttccgtGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGa                                                             >  1:627331/1‑150 (MQ=255)

                                                                       tccgtGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAg                                                           <  2:434999/150‑1 (MQ=255)

                                                                         cgtGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAgcgg                                                        >  1:649153/1‑151 (MQ=255)

                                                                            ggCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGa                                                     <  2:86218/151‑1 (MQ=255)

                                                                                  aaGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTa                                               <  1:185198/151‑1 (MQ=255)

                                                                                        tgCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCt                                          >  2:599990/1‑150 (MQ=255)

                                                                                           aaaCCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAgcg                                      >  2:220462/1‑151 (MQ=255)

                                                                                             aCCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCa                                    <  1:19941/151‑1 (MQ=255)

                                                                                              ccGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCaa                                   <  1:351327/151‑1 (MQ=255)

                                                                                                 aagaaACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAAtt                                >  2:187204/1‑151 (MQ=255)

                                                                                                    aaaCCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTaa                              >  2:53722/1‑150 (MQ=255)

                                                                                                      aCCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTaa                              <  1:605702/148‑1 (MQ=255)

                                                                                                      aCCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTaa                              >  2:605708/1‑148 (MQ=255)

                                                                                                       ccGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGgc                          <  2:506632/151‑1 (MQ=255)

                                                                                                          aTAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCt                       <  2:347802/151‑1 (MQ=255)

                                                                                                            aGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTAc                     >  1:653716/1‑151 (MQ=255)

                                                                                                                      tATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCg           >  1:23494/1‑151 (MQ=255)

                                                                                                                           tAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGt      >  1:614013/1‑151 (MQ=255)

                                                                                                                            aaTCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATgg       <  2:121004/149‑1 (MQ=255)

                                                                                                                               cGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGa  <  2:207318/151‑1 (MQ=255)

GCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGA  >  NC_000913/3788811‑3789088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A