Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 193 39.9 1341687 91.8% 1231668 149.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,790,211 T→C intergenic (‑130/+109) waaH ← / ← tdh LPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,790,2110TC100.0% 148.6 / NA 39intergenic (‑130/+109)waaH/tdhLPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/26);  total (13/26)

GGTGCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCG  >  NC_000913/3790067‑3790357
                                                                                                                                                |                                                                                                                                                  
ggTGCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATaa                                                                                                                                              <  2:264086/151‑1 (MQ=255)
   gCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAagag                                                                                                                                           <  2:318835/151‑1 (MQ=255)
   gCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAaga                                                                                                                                            <  1:285331/150‑1 (MQ=255)
        tcatcaTAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCa                                                                                                                                       <  2:492061/150‑1 (MQ=255)
           tcatAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGcac                                                                                                                                   >  2:474522/1‑151 (MQ=255)
            catAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGcaca                                                                                                                                  <  1:358176/151‑1 (MQ=255)
            catAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGcac                                                                                                                                   <  2:184621/150‑1 (MQ=255)
                aaTTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAAtt                                                                                                                               >  2:132414/1‑150 (MQ=255)
                aaTTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTa                                                                                                                              <  1:93592/151‑1 (MQ=255)
                  ttACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACg                                                                                                                            >  2:146778/1‑151 (MQ=255)
                        acgacCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAActc                                                                                                                       <  2:618479/150‑1 (MQ=255)
                                  aaGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACt                                                                                                            <  1:151534/151‑1 (MQ=255)
                                      tATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGAc                                                                                                         >  2:632747/1‑150 (MQ=255)
                                       aTCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACa                                                                                                        <  2:37383/150‑1 (MQ=255)
                                       aTCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACa                                                                                                        <  1:629801/150‑1 (MQ=255)
                                       aTCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAg                                                                                                       <  2:644678/151‑1 (MQ=255)
                                           tGACGTTTTACAGGCCTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGAtt                                                                                                   >  1:335210/1‑151 (MQ=255)
                                               gTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTAc                                                                                               >  2:232493/1‑151 (MQ=255)
                                                      aGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTttatt                                                                                                                 >  1:467500/1‑126 (MQ=255)
                                                      aGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTttatt                                                                                                                 <  2:467504/126‑1 (MQ=255)
                                                              caTTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTa                                                                                <  2:309641/150‑1 (MQ=255)
                                                               aTTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTa                                                                                <  2:667535/150‑1 (MQ=255)
                                                                 tAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATcc                                                                             >  2:109665/1‑151 (MQ=255)
                                                                  aaGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCt                                                                            <  1:405896/151‑1 (MQ=255)
                                                                      aaCTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGa                                                                        <  1:115372/151‑1 (MQ=255)
                                                                              aGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAggg                                                                <  1:624795/151‑1 (MQ=255)
                                                                              aGCCTGTGAGATATTATTTTTACAGGCGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAggg                                                                >  1:262528/1‑151 (MQ=255)
                                                                                ccTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCt                                                              <  1:427078/151‑1 (MQ=255)
                                                                                     gagaTATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAAt                                                         <  2:375744/151‑1 (MQ=255)
                                                                                       gaTATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATAc                                                       <  1:461413/151‑1 (MQ=255)
                                                                                                   aCAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTc                                           <  2:20833/151‑1 (MQ=255)
                                                                                                          agagagTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTaa                                    <  1:663476/151‑1 (MQ=255)
                                                                                                                   cTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTc                           >  1:164870/1‑151 (MQ=255)
                                                                                                                     tCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTc                           <  2:126343/149‑1 (MQ=255)
                                                                                                                          gTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATaa                    <  2:159265/151‑1 (MQ=255)
                                                                                                                              agagaTAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACttt                >  1:151678/1‑151 (MQ=255)
                                                                                                                                        agagCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTgg      >  1:666046/1‑151 (MQ=255)
                                                                                                                                          agcagcCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGcc    <  2:501047/151‑1 (MQ=255)
                                                                                                                                            cagcCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCg  >  1:263155/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GGTGCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCG  >  NC_000913/3790067‑3790357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A