Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,828,150	G→A	G172G (GGC→GGT)	gltS ←	glutamate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,828,150	0	G	A	100.0%	106.5 / NA	29	G172G (GGC→GGT)	gltS	glutamate transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/14); total (15/14)

ATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCA  >  NC_000913/3828004‑3828292

aTAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCAccc                                                                                                                                             >  2:323966/1‑150 (MQ=255)

aTAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCa                                                                                                                                            <  2:666529/151‑1 (MQ=255)

      tCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCAcc                                                                                                                                       >  1:641322/1‑150 (MQ=255)

               aCCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACg                                                                                                                             >  2:521143/1‑151 (MQ=255)

                    cGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGca                                                                                                                        <  2:298791/151‑1 (MQ=255)

                          gATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGcc                                                                                                                  <  2:262437/151‑1 (MQ=255)

                             cATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCAt                                                                                                                <  2:293296/150‑1 (MQ=255)

                                 cGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGcc                                                                                                            >  1:297593/1‑150 (MQ=255)

                                 cGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTAGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGcc                                                                                                            >  2:384789/1‑150 (MQ=255)

                                    cccACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACt                                                                                                         <  2:497626/150‑1 (MQ=255)

                                           ccGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCg                                                                                                  <  2:288397/150‑1 (MQ=255)

                                                 tttCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTgg                                                                                            <  1:520010/150‑1 (MQ=255)

                                                         gCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCAt                                                                                   <  2:467889/151‑1 (MQ=255)

                                                           cGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATa                                                                                  >  2:323878/1‑150 (MQ=255)

                                                              cGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACg                                                                               <  2:144533/150‑1 (MQ=255)

                                                                     tCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATg                                                                        <  1:567349/150‑1 (MQ=255)

                                                                                    aTACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACg                                                        <  1:323876/151‑1 (MQ=255)

                                                                                         gTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAgcg                                                   >  1:186363/1‑151 (MQ=255)

                                                                                                gtggtgGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTAc                                            >  2:52261/1‑151 (MQ=255)

                                                                                                                 cAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGaa                              >  2:360271/1‑148 (MQ=255)

                                                                                                                 cAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAaga                           <  2:376162/151‑1 (MQ=255)

                                                                                                                             cACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCg               >  2:490875/1‑151 (MQ=255)

                                                                                                                              aCCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCgg              >  2:514169/1‑151 (MQ=255)

                                                                                                                              aCCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCg               >  1:527283/1‑150 (MQ=255)

                                                                                                                                cGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGcc            <  2:216620/151‑1 (MQ=255)

                                                                                                                                      gCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGc      >  2:576113/1‑151 (MQ=255)

                                                                                                                                        cAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGccc    >  2:470947/1‑151 (MQ=255)

                                                                                                                                          aTCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGcccc   >  2:54237/1‑150 (MQ=255)

                                                                                                                                          aTCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCa  <  1:388158/151‑1 (MQ=255)

ATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCA  >  NC_000913/3828004‑3828292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A