Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 193 39.9 1341687 91.8% 1231668 149.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,914,638 G→C T188S (ACC→AGC)  glmU ← fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,914,6380GC100.0% 159.7 / NA 43T188S (ACC→AGC) glmUfused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (23/20);  total (23/20)

CCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGCTTAAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTC  >  NC_000913/3914495‑3914782
                                                                                                                                               |                                                                                                                                                
ccAGACGGGAGAGTTGCAGGCGGTTAGTCACGCCTTCTACTTCGCTTAAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCtt                                                                                                                                           <  1:405269/151‑1 (MQ=255)
   gACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGCTTAAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCgc                                                                                                                                        <  2:137622/151‑1 (MQ=255)
          gagTTGCAGGCGGTTATTCACGCCTTCTACTTCGCTTAAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGGGATGTAGTATTTGCCCTGAGCATTATTGTTGCTCAGCTTCgccagcc                                                                                                                                   >  2:331443/1‑149 (MQ=255)
                        tATTCACGCCTTCTACTTCGCTTAAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTg                                                                                                                    <  2:592412/150‑1 (MQ=255)
                               gCCTTCTACTTCGCTTAAACGGTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGAAACGCCAGCGCAATAATGTAGGTGATGTAGTATTCCCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGtt                                                                                                             >  1:487342/1‑150 (MQ=255)
                                      aCTTCGCTTAAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCaa                                                                                                        <  2:506187/148‑1 (MQ=255)
                                      aCTTCGCTTAAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAAt                                                                                                       <  1:647951/149‑1 (MQ=255)
                                         tCGCTTAAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGa                                                                                                   >  1:266940/1‑150 (MQ=255)
                                          cGCTTAAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGaa                                                                                                  <  2:376484/150‑1 (MQ=255)
                                          cGCTTAAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAAt                                                                                                 >  1:357544/1‑151 (MQ=255)
                                                      gCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGAt                                                                                     <  2:106988/151‑1 (MQ=255)
                                                       cGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGAtc                                                                                    <  1:155375/151‑1 (MQ=255)
                                                         gATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGAtctc                                                                                  >  2:544398/1‑151 (MQ=255)
                                                         gATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTACTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGAtctc                                                                                  >  2:488258/1‑151 (MQ=255)
                                                          aTGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTcc                                                                                 >  1:470787/1‑151 (MQ=255)
                                                              aCGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCTGGTGTTGATCTCCTGaa                                                                             <  2:666283/151‑1 (MQ=255)
                                                              aCGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGa                                                                              >  2:483917/1‑150 (MQ=255)
                                                                 gCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCGGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCt                                                                          <  1:597672/151‑1 (MQ=255)
                                                                         ttCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACgctgc                                                                  >  2:179059/1‑151 (MQ=255)
                                                                           cACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCtc                                                                <  2:324292/151‑1 (MQ=255)
                                                                             cGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCtcgt                                                              >  2:232872/1‑151 (MQ=255)
                                                                                     cTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGc                                                       >  2:249535/1‑150 (MQ=255)
                                                                                       gATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCa                                                      <  2:343032/149‑1 (MQ=255)
                                                                                       gATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCa                                                      <  1:483915/149‑1 (MQ=255)
                                                                                            gCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTtg                                                >  1:264468/1‑150 (MQ=255)
                                                                                                 cgcAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTc                                           >  1:469911/1‑150 (MQ=255)
                                                                                                  gcAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTcaa                                         <  2:236142/151‑1 (MQ=255)
                                                                                                     ataatGTCGGTTATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGGTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTcaacaa                                      <  2:566389/151‑1 (MQ=255)
                                                                                                     ataatGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTcaacaa                                      <  1:217257/151‑1 (MQ=255)
                                                                                                      taatGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAAt                                     >  1:80899/1‑151 (MQ=255)
                                                                                                      taatGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAAt                                     >  2:84359/1‑151 (MQ=255)
                                                                                                       aatGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATg                                    <  2:251580/151‑1 (MQ=255)
                                                                                                       aatGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATg                                    >  2:10248/1‑151 (MQ=255)
                                                                                                       aatGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGGGGCATCTTTGTGCTCAACAATg                                    >  1:69333/1‑151 (MQ=255)
                                                                                                            cGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGt                               >  2:339973/1‑151 (MQ=255)
                                                                                                                 aTGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACtt                          >  2:200776/1‑151 (MQ=255)
                                                                                                                  tGTAGTATTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACttt                         >  1:26996/1‑151 (MQ=255)
                                                                                                                     agtaTTCGCCCTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGcc                      >  2:638593/1‑151 (MQ=255)
                                                                                                                               cTGAGCATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACgg            >  1:19702/1‑151 (MQ=255)
                                                                                                                                    cATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGAt       <  2:574967/151‑1 (MQ=255)
                                                                                                                                    cATTATTGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGAt       >  1:504878/1‑151 (MQ=255)
                                                                                                                                     attattGTTGCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATa      <  1:232872/151‑1 (MQ=255)
                                                                                                                                          tgttgCTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTc  <  2:172611/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGCTTAAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGCGTTTCATATCTGCGCCGTTGGCAATCAGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTC  >  NC_000913/3914495‑3914782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A