Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,342,193	A→G	T95A (ACG→GCG)	melA →	alpha‑galactosidase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,342,193	0	A	G	97.5%	122.7 / NA	40	T95A (ACG→GCG)	melA	alpha‑galactosidase, NAD(P)‑binding
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (19/20); minor base T (1/0); total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

GGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGAAG  >  NC_000913/4342048‑4342328

ggAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAGCTTGCGCgggg                                                                                                                                    >  1:202589/1‑149 (MQ=255)

ggAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTTCGCgggg                                                                                                                                    >  2:452532/1‑149 (MQ=255)

ggAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCgg                                                                                                                                      <  1:452527/149‑1 (MQ=255)

         gCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACTTGCGCGGGGACTGAttt                                                                                                                            >  1:593880/1‑150 (MQ=255)

            tatTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACTTGCGCGGTGAATGAt                                                                                                                              >  1:472966/1‑145 (MQ=255)

              ttGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAgg                                                                                                                       <  1:100332/150‑1 (MQ=255)

               tgtgGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGt                                                                                                                      <  2:598622/150‑1 (MQ=255)

                     gCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTaa                                                                                                                <  2:115111/150‑1 (MQ=255)

                       gTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCg                                                                                                             <  1:587365/151‑1 (MQ=255)

                          aGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTTCGCGGGGACTGATTTTGAGGTCTGTAAGCgg                                                                                                            >  2:570176/1‑149 (MQ=255)

                               aTGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTc                                                                                                     <  1:607548/151‑1 (MQ=255)

                                   aTTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTgg                                                                                                  <  1:63189/150‑1 (MQ=255)

                                         cagGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGGGACTGATTTCGAGGTATGTAAGGGGGAAGGGCTGGAACaaa                                                                                            >  2:645328/1‑150 (MQ=255)

                                                 aGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTTCGCGGGGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCa                                                                                         >  1:643092/1‑145 (MQ=255)

                                                    ggCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATa                                                                                <  1:324632/151‑1 (MQ=255)

                                                    ggCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGGCTGTAAGCGGCATGGGCTGGGACAAAACCTTGcc                                                                                    >  2:24495/1‑147 (MQ=255)

                                                     gCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGGGACTGATTTCGAGGGCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGAt                                                                                 >  2:420044/1‑149 (MQ=255)

                                                            cACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTggg                                                                         <  1:501568/150‑1 (MQ=255)

                                                                 gCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCgg                                                                    <  1:289694/150‑1 (MQ=255)

                                                                 gCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCgg                                                                    <  2:204845/150‑1 (MQ=255)

                                                                            cAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGTGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAGCCATTGCCGATACGTTGGGGCCGGGCGGtattat                                                          <  1:303796/149‑1 (MQ=255)

                                                                             agaaagaaGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATgcg                                                       <  1:577518/151‑1 (MQ=255)

                                                                             agaaagaaGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCCTGCGCGGTGACTGATTTCGGGGTCTGTAAGCGGCATGGGCTGGGACAAAACATTGCCGATACGTTGGGGCCGGGGGGgattat                                                          >  2:278308/1‑148 (MQ=255)

                                                                                aagaagCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATTAACCTTGCGCGGGGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAAACATTGCCGATACGTTGGGGCCGGGCGGTATTATgcgcg                                                     >  2:559797/1‑150 (MQ=255)

                                                                                  gaagCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATTAAACCTGTGCGGGGGCTGGTTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAATGCCGGTAAGTTGGGGCCGGGCGGGATTATgcgcgc                                                    >  1:263942/1‑149 (MQ=255)

                                                                                     gCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTAc                                               <  2:384470/151‑1 (MQ=255)

                                                                                      ccTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACCTGCGCGGGGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGGCGGGCGGTATTATGCGCGCGCTAc                                               >  2:86623/1‑150 (MQ=255)

                                                                                       cTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACTTGCGCGGGGGCTGGTTTTGAGGTCTGTAAGCGGCATGGTCTGGAAAAAACCATTGCCGATAAGTTGGGGCCGGGGGGGATTATGGGCGCGCTAc                                               >  1:571824/1‑149 (MQ=255)

                                                                                          agagGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTAc                                           <  1:492082/150‑1 (MQ=255)

                                                                                                 gCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTTTGAACCTTTCTCGGTGACCGGTTTCTCGGTCTGTAAGGGGCATGGTCTGGAACAAACCACTTCCCATACGTTGGGGCCGGGGGGGATTATGCCGGCGCTACGCAcc                                          >  1:56540/1‑144 (MQ=255)

                                                                                                       tttGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCtg                              <  2:530705/150‑1 (MQ=255)

                                                                                                               ggtggCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCa                                  <  2:279845/138‑1 (MQ=255)

                                                                                                               ggtggCATTTCAGATTGGCGGTTATGAAACTTGCGCGGGGACTGATTTTGAGGTCTGTAAGCGGCATGGTCTGGAACAAAACATTGCCGATACGGTGGGGGCGGGGGGGATTATGCGCGCGCTACGTACCATTTCGc                                   >  1:279846/1‑137 (MQ=255)

                                                                                                                gtggCATTTCAGATTGGCGGTTATGAAACTTGCGCGGGGAATGATTTTGAGGTCTGTAAGCGGCAAGGTCTGGAAAAAACCATTGCCGATAAGTTGGGGCCGGGCGGGATTATGCGCCCGCTACGTACCATTCCCCATCTGTGGCAAAttt                    >  2:148905/1‑151 (MQ=255)

                                                                                                                    cATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGa                <  2:76722/151‑1 (MQ=255)

                                                                                                                     aTTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAg               <  2:339855/151‑1 (MQ=255)

                                                                                                                                 cGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGa    >  2:340496/1‑150 (MQ=255)

                                                                                                                                  ggTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGa    >  2:54336/1‑149 (MQ=255)

                                                                                                                                  ggTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGa    >  2:172092/1‑149 (MQ=255)

                                                                                                                                  ggTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGAAg  <  1:232514/151‑1 (MQ=255)

GGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGAAG  >  NC_000913/4342048‑4342328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A