Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I1 R1 144 141.8 4677362 93.6% 4378010 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,342,193 A→G T95A (ACG→GCG)  melA → alpha‑galactosidase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,342,1930AG96.9% 395.7 / ‑5.7 130T95A (ACG→GCG) melAalpha‑galactosidase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (55/71);  minor base C (4/0);  total (59/71)
Fisher's exact test for biased strand distribution p-value = 4.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

Only 100 of 130 total aligned reads displayed.

TGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGAAGTCTGCCCCGAT  >  NC_000913/4342047‑4342339
                                                                                                                                                  |                                                                                                                                                  
aggAAGAGTCGCATATTGTGGTGCGTAAGATGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGCTATTAACCGTGCGCggg                                                                                                                                                >  1:1295792/2‑150 (MQ=255)
 ggAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGt                                                                                                                                                <  1:716476/150‑1 (MQ=255)
 ggAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTg                                                                                                                                               <  1:1112824/151‑1 (MQ=255)
    agagTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACt                                                                                                                                            >  2:486873/1‑151 (MQ=255)
    agagTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGCGGTGGCATTTCAGATTGGCGGTTATGAACCTTCCGCGGTggc                                                                                                                                             >  4:53034/1‑148 (MQ=255)
     gagTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATTGACCCCGCGCGCTGACTg                                                                                                                                           >  1:481142/1‑151 (MQ=255)
       gTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATTAAACTTTCGCggggggtgat                                                                                                                                         >  1:156061/1‑143 (MQ=255)
             tatTGTGGTGGGAAAGCTGATGGATTCGGCAGGGGCCAGCGGCAAAATAACCTGCCGCACCCAACAGAAAGAAGCCGTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAGCCTTGCGCGGTGACTGAttt                                                                                                                                       <  2:1321501/147‑1 (MQ=255)
             tatTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTTTGAAACCTGCGCGGTGAATGAttt                                                                                                                                       >  3:536566/1‑147 (MQ=255)
             tatTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGGGTCTGATTTCggg                                                                                                                                   >  1:476232/1‑149 (MQ=255)
             tatTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACTTTCGCGGGGGCTGAttt                                                                                                                                       >  1:1599682/1‑147 (MQ=255)
              atTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAgg                                                                                                                                  <  1:1183998/151‑1 (MQ=255)
              atTGAGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATAACCTGCCACACCCAACAGAAAGAAGCCTGAGAGGATGCCGATTTTGTCGGGGTGGCATTTCAGATTGGCGGCTATAAACATTGCGGGGTGaaatgttt                                                                                                                                       >  1:1429134/1‑139 (MQ=255)
                     tgCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACTTGCGCGGTGACTGATTTTGAggggtg                                                                                                                              >  2:430533/1‑144 (MQ=255)
                      gCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAg                                                                                                                          <  2:978806/151‑1 (MQ=255)
                       cGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGTGCGGGGACTGGTTTTGAGGTCTGTa                                                                                                                            >  1:1248090/1‑148 (MQ=255)
                       cGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGc                                                                                                                         <  2:311448/151‑1 (MQ=255)
                       cGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGGTATGAACCTTTCGCGGGGGCTGATTTTGGGGTCTGTaa                                                                                                                           >  1:701224/1‑149 (MQ=255)
                       cGTAAGCTGATGGATTCAGCAGGAGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGGGACTGATTTCGAGGGTTGTaa                                                                                                                           >  2:82366/1‑149 (MQ=255)
                         tAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCgg                                                                                                                       >  3:215039/1‑151 (MQ=255)
                            gCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTTTGAACCTTTCGCGGGGGCTGGTTTTGAGGTCTGTAAGCGGCAt                                                                                                                    >  2:703218/1‑151 (MQ=255)
                            gCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTTCGCGGGGGATGATTTCGAgggagatcgg                                                                                                                          >  4:180080/1‑137 (MQ=255)
                            gCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCAt                                                                                                                    >  3:128655/1‑151 (MQ=255)
                             cTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGt                                                                                                                                 <  3:180100/137‑1 (MQ=255)
                               gATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATgg                                                                                                                  <  2:604704/150‑1 (MQ=255)
                               gATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATgg                                                                                                                  <  2:1119498/150‑1 (MQ=255)
                                aTGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTGATTAGACCTGCGGGGGGACTGATTTCCAGGGTTGTAAGAggagt                                                                                                                    >  1:746303/1‑144 (MQ=255)
                                  ggATTCAGCAGGGGCCAGCGGCAAAATCCCCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCt                                                                                                               <  1:1634506/150‑1 (MQ=255)
                                  ggATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTg                                                                                                              <  3:347052/151‑1 (MQ=255)
                                   gATTAAGCGGGGGCCAGCGGCAAAATCCCCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTgg                                                                                                             <  1:565192/151‑1 (MQ=255)
                                      tCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGaa                                                                                                           <  2:1522699/150‑1 (MQ=255)
                                       cagcagGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATTAACCTTTCGCGGGGGCTGATTTCGAGGTCTGTAAACGGCATGGTCTggga                                                                                                           >  2:1191323/1‑147 (MQ=255)
                                        agcagGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTAATAACCCTTCCCTGTGGCTGATTTCGGCGGCCGTAAGCGGCCTGGGCCGGaaaa                                                                                                         >  1:1575433/1‑148 (MQ=255)
                                          cagGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACTTGCGCGGGGACTGGTTTTCAGGGCTTTAAGAGGCAAGGTGTGGGACaaa                                                                                                       >  1:1599820/1‑150 (MQ=255)
                                           agGGGCCAGGGGAAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAAc                                                                                                      <  1:1702356/150‑1 (MQ=255)
                                           agGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGGGGATGATTTTGAGGTTTGTTAAGGGCATGGTGTTGGACAAAcc                                                                                                     >  4:121079/1‑151 (MQ=255)
                                            ggggCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCCTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCa                                                                                                    <  2:417676/151‑1 (MQ=255)
                                                ccAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGc                                                                                                <  1:602717/151‑1 (MQ=255)
                                                ccAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGc                                                                                                <  2:938959/151‑1 (MQ=255)
                                                ccAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGc                                                                                                <  2:274240/151‑1 (MQ=255)
                                                     ggCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGAt                                                                                            <  2:1314287/150‑1 (MQ=255)
                                                     ggCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGAt                                                                                            <  1:1306272/150‑1 (MQ=255)
                                                        aaaaTCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGt                                                                                        <  1:329321/151‑1 (MQ=255)
                                                        aaaaTCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGGTAAGAACACTGCGCCGTGACTGTTTTTCAGGGCTGCAAAGAGCAAGGGATCGAAAAAACCATCTTCGATACg                                                                                         >  1:286673/1‑150 (MQ=255)
                                                          aaTCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTg                                                                                      <  2:934569/151‑1 (MQ=255)
                                                             cACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTggg                                                                                    <  4:382202/150‑1 (MQ=255)
                                                               ccTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGcc                                                                                 <  2:879286/151‑1 (MQ=255)
                                                                   ccACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGc                                                                             <  1:491856/151‑1 (MQ=255)
                                                                      cacCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCgg                                                                           <  1:440014/150‑1 (MQ=255)
                                                                          cAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACTTGCGCGGGGACTGATTTCGAGGGCTGTAAGCGGCCTGGGCTGGGACAAACCATTGCCGATAAGGTGGGGGCGGGCGGgatt                                                                       >  3:479123/1‑150 (MQ=255)
                                                                           aaCAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGAGCCGGGCGGtattat                                                                     <  1:699984/151‑1 (MQ=255)
                                                                            aCAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATg                                                                    <  3:381500/151‑1 (MQ=255)
                                                                            aCAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACCTACGCGGGGAATGTTTTCGTGGTCAGTAAGCGGCATGGTCTGGAAAAAACCATTGCCGACATGCTGTGGGCGGGCGGtact                                                                       >  1:1412621/1‑146 (MQ=255)
                                                                              agaaagaaGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTTTTTACCATGAGCCGTGATTTCTTTCGAGGGCTGTAAGGCGGATGGTCTGGGACAAAACAATTCCCGTACGGTTGGGCCTGGGGGGGTTATgcg                                                                  >  1:1568728/1‑151 (MQ=255)
                                                                                aaagaaGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATgcgcg                                                                <  2:434467/151‑1 (MQ=255)
                                                                                aaagaaGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGGTTTGAAACCTTCGCGGGGGATGGATTCGAGGGCTGTAAGCGGCCTGGGCTGGGAAAAAACACTTCCCATCCGTTGGGGCCGGGCGGgattat                                                                     >  1:488217/1‑146 (MQ=255)
                                                                                 aagaagCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATTAACCTTTCGCGGGGACTGATTTCGAGGTCTGTAAGCGGCATGGGCTGGGACAAAACATTGCCGGTACGTTGGGGGCGGGGGGGATTTTgcgcg                                                                >  2:583380/1‑150 (MQ=255)
                                                                                   gaagCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATgcgcgcgc                                                             <  4:282823/151‑1 (MQ=255)
                                                                                    aagCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCCTGCGCGGGGGCTGATTTTGAGGGCTGTAAGCGGCATGGGCTGGGACAAAACATTTGCGATACGTTTGGGCCGGGCGGGATTTTgcgcgcg                                                              >  2:1158645/1‑149 (MQ=255)
                                                                                     agCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGTGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTa                                                           <  1:1062884/151‑1 (MQ=255)
                                                                                     agCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTa                                                           <  2:1496816/151‑1 (MQ=255)
                                                                                     agCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTa                                                           <  1:1401182/151‑1 (MQ=255)
                                                                                      gCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTa                                                           <  1:1047888/150‑1 (MQ=255)
                                                                                      gCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGGGACTGATTTTGAGGGCTGTAAGCGGCATGGTCTGGAACAAAACATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTa                                                           >  2:49536/1‑150 (MQ=255)
                                                                                       ccTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACCTGCGCGGTGACTGGTTTCGAGGTCTGTAAGCGGCATGGGCTGGGACAAAACATTGCCGATACGGTGGGGCCGGGCGGTATTATgcgcg                                                                >  4:360462/1‑144 (MQ=255)
                                                                                         ttAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTTGAGGGCTGTAAGCGGCATGGGCTGGGACAAAACATTGCCGATACGTTGGGGCCGGGGGGGATTATGCGCGCGCTAc                                                          >  4:509169/1‑148 (MQ=255)
                                                                                         ttAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGGCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGAAACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACg                                                         >  3:129869/1‑149 (MQ=255)
                                                                                          tAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATTAAACTTTCGCGGGGACTGATTTTGAGGGCTGTAAGCGGCATGGTCTGGGACAAACCATTGCCGATACGTTGGGGGCGGGCGGGATTATTCGCGCGCTACGTa                                                       >  2:1378432/1‑150 (MQ=255)
                                                                                          tAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTa                                                       <  1:933251/150‑1 (MQ=255)
                                                                                               gATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTc                                                 <  1:1650699/151‑1 (MQ=255)
                                                                                                 tGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTTTTAACCTTGCGCGGGGACGGCTTTAGAGGGGTGTTAGCGGGGTGGGGTGGGATCAACCACTGGCGAAAAGTTTGGGCCGGGGGGTATTTTGCGCGCGGCACCTGCCAtt                                                  >  1:864366/1‑148 (MQ=255)
                                                                                                  gCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCg                                               <  1:1265494/150‑1 (MQ=255)
                                                                                                  gCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGc                                              <  2:270871/151‑1 (MQ=255)
                                                                                                       ttttGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTTCGCGGTGGCTGATTTCGAGGTCTGTAAGCGGCATGGGCTGGGACAAACCATTTCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCCACGTACCATTCCGCATCt                                          >  4:24370/1‑150 (MQ=255)
                                                                                                        tttGTCGTGGTGGCATTTCAGATTGGCGGTTTTGAACCTTGCGCGGGGGCTTATTTCGAGGGCTGTAAGCGGGATGGGCTGGGAAAAAACATTGGCGATACGTTGGGGGCGGGCGGGAATATGTGCGCGCCACGTACCAATTCGCATCt                                          >  3:340142/1‑149 (MQ=255)
                                                                                                        tttGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACTTTCGCGGGGGCTGATTTTGAGGGCTGTAAGGGGCAAGGGTTGGAAAAAAACATTTCCGATAAGTTGGGGCCGGGGGGGATTATTGGCGCGCCACGTACCATTCCCCaac                                           >  1:870775/1‑146 (MQ=255)
                                                                                                        tttGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACCTTCGCGGTGACTGATTTTGAGGGCTGTAAGCGGCCTGGTCTGGAACAAACCCTTCCCGATACGTTTGGGCCGGGGGGGATTATTCGCGGGCTACGTACCATTTCGc                                              >  1:1663146/1‑145 (MQ=255)
                                                                                                         ttGTCGTGGTGGCATTTCAGATTGGCGGGTATGAAACTTGGGCGGTGGATGATTTCGAGGGATGTAAGGGGCAAGGGAAGGAACAAAACAAAGCCGAAAAAGTGGGGGCGGGGGGGAGTAATCGCACGCGACGTTCCAATCAGccact                                          >  4:248600/1‑144 (MQ=255)
                                                                                                           gTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTgg                                      <  1:1135454/150‑1 (MQ=255)
                                                                                                            tCGTGGTGGCATTTCAGATTGGCGGTTATGGACCTTTCCCCTGGCCCTAATTCTAGGGGTGTAAGGGGCGTGGCATCGGACGAACCAATGCCGATTCGTTTGGGCCGGGCGGTGATATGCCAGGGCTACCTACCCTTGCGCCTcattgg                                      >  2:320175/1‑144 (MQ=255)
                                                                                                             cGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCaa                                   <  2:754980/151‑1 (MQ=255)
                                                                                                               tggtggCATTTCAGATTGGCGGTTATGAAACTTGCGCGGGGACTGATTTTGAGGGCTGTAAGCGGCATGGTCTGGAAAAAACCATTGCCGATACGTTGGGGGCGGGCGGGATTATGCGCGCGCTACGTACCATTCCGCCTCTGTGGCaaa                                  >  4:244304/1‑150 (MQ=255)
                                                                                                                ggtggCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAAtt                                <  2:1540764/151‑1 (MQ=255)
                                                                                                                 gtggCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAAttt                               <  2:612433/151‑1 (MQ=255)
                                                                                                                   ggCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTg                              <  4:513238/150‑1 (MQ=255)
                                                                                                                         tCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGa                        <  3:304116/150‑1 (MQ=255)
                                                                                                                          cAGATTGGCGGTTATGAACCTTGCGCGGGGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGGGGGATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAgg                         >  4:386500/1‑148 (MQ=255)
                                                                                                                           aGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACAt                     <  4:129849/151‑1 (MQ=255)
                                                                                                                            gATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATg                    <  1:1191190/151‑1 (MQ=255)
                                                                                                                             aTTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGa                   <  2:1109317/151‑1 (MQ=255)
                                                                                                                               tGGCGGGTAAGAACCTTGCCCGGGGTCTGATTTCGAACTCCGTAAGCGGCATGGTCTGGAACAAACCATTGCCGGTACGTTCCGGCCCCGCGGTATTATGCGCGCGCTACGGACCATTCCGCCTCTGTGGCAAATTTGCGAGGACATGCCg                 >  1:262566/1‑151 (MQ=255)
                                                                                                                                 gCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGa               <  2:1248234/151‑1 (MQ=255)
                                                                                                                                   ggTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGa               >  2:1513055/1‑149 (MQ=255)
                                                                                                                                    gTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGAAGt            <  4:370719/151‑1 (MQ=255)
                                                                                                                                      tATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGAAGTc           <  2:1131423/150‑1 (MQ=255)
                                                                                                                                       aTGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGAAGTCTg         <  2:1599842/151‑1 (MQ=255)
                                                                                                                                        tGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGAAGTCTGc        >  1:1071693/1‑151 (MQ=255)
                                                                                                                                            ccTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGAAGTCTGcccca    <  1:1042826/151‑2 (MQ=255)
                                                                                                                                               tGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCGAATTTGCGAGGACATGACGGAAGTCTGCCCCGAt  <  2:651942/150‑1 (MQ=255)
                                                                                                                                               tGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGAAGTCTGCCCCGAt  <  4:276687/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGAAGTCTGCCCCGAT  >  NC_000913/4342047‑4342339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A