Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,453,330	A→G	N254D (AAC→GAC)	ytfT →	putative sugar ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,453,330	0	A	G	100.0%	132.1 / NA	37	N254D (AAC→GAC)	ytfT	putative sugar ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/15); total (22/15)

GTATCAACATTCGGGCGGCAAAAAATGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTC  >  NC_000913/4453196‑4453475

gTATCAACATTCGGGCGGCAAAAAATGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATg                                                                                                                                   <  1:451795/151‑1 (MQ=255)

  aTCAACATTCGGGCGGCAAAAAATGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATgg                                                                                                                                  <  1:352460/150‑1 (MQ=255)

    cAACATTCGGGCGGCAAAAAATGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGgct                                                                                                                                <  2:580971/150‑1 (MQ=255)

                  cAAAAAATGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCAt                                                                                                                 <  2:616984/151‑1 (MQ=255)

                    aaaaaTGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCAtt                                                                                                                >  1:108600/1‑150 (MQ=255)

                    aaaaaTGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCAtt                                                                                                                >  1:438048/1‑150 (MQ=255)

                     aaaaTGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATtct                                                                                                              >  2:612680/1‑151 (MQ=255)

                              gggTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                                                                     >  1:317060/1‑151 (MQ=255)

                                  aaaCACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGAtt                                                                                                 >  1:61446/1‑151 (MQ=255)

                                    acacGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTgg                                                                                               >  2:373045/1‑151 (MQ=255)

                                     cacGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTggc                                                                                              <  1:74608/151‑1 (MQ=255)

                                              catcGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCg                                                                                     >  2:498851/1‑151 (MQ=255)

                                              catcGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCg                                                                                     >  2:153310/1‑151 (MQ=255)

                                                tcGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGc                                                                                    <  1:545680/150‑1 (MQ=255)

                                                   tCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGAt                                                                                >  2:275443/1‑151 (MQ=255)

                                                    cATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATg                                                                               <  1:6251/151‑1 (MQ=255)

                                                              tATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGgcgc                                                                      >  1:423433/1‑150 (MQ=255)

                                                               aTGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCt                                                                     <  2:391079/150‑1 (MQ=255)

                                                                 gtgtTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCttt                                                                   <  1:359975/150‑1 (MQ=255)

                                                                  tgtTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCttt                                                                   >  1:106183/1‑149 (MQ=255)

                                                                   gtTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAAc                                                                <  2:438053/151‑1 (MQ=255)

                                                                     tGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAAcc                                                               <  2:125326/150‑1 (MQ=255)

                                                                     tGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAAcc                                                               <  2:209835/150‑1 (MQ=255)

                                                                      gAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTg                                                             >  1:522108/1‑151 (MQ=255)

                                                                           ggCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACt                                                        >  1:458767/1‑151 (MQ=255)

                                                                                     ggcgATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGCTGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGgtggtg                                              >  2:607610/1‑151 (MQ=255)

                                                                                        gATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTgggg                                           >  1:299852/1‑151 (MQ=255)

                                                                                                      tCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGattatt                               >  2:296820/1‑149 (MQ=255)

                                                                                                         tGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAggg                          >  1:430987/1‑151 (MQ=255)

                                                                                                             ggcggATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATg                      >  2:221469/1‑151 (MQ=255)

                                                                                                             ggcggATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATg                      >  2:553841/1‑151 (MQ=255)

                                                                                                             ggcggATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATg                      <  2:106186/151‑1 (MQ=255)

                                                                                                               cggATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGa                     >  2:184458/1‑150 (MQ=255)

                                                                                                                       cgcgGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGaa            <  1:236450/151‑1 (MQ=255)

                                                                                                                        gcgGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAAt           >  1:596333/1‑151 (MQ=255)

                                                                                                                          ggTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAAttt         >  1:508719/1‑151 (MQ=255)

                                                                                                                                 aTGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTc  <  2:278943/151‑1 (MQ=255)

GTATCAACATTCGGGCGGCAAAAAATGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTC  >  NC_000913/4453196‑4453475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A