Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,838	T→C	G179G (GGA→GGG)	ldtC ←	L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,838	0	T	C	100.0%	130.9 / NA	37	G179G (GGA→GGG)	ldtC	L,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/21); total (16/21)

GATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACC  >  NC_000913/1169700‑1169984

gATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCAc                                                                                                                                         >  1:606640/1‑150 (MQ=255)

gATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACt                                                                                                                                        <  2:117400/151‑1 (MQ=255)

   atCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTAcc                                                                                                                                     >  1:586332/1‑151 (MQ=255)

      gTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGACGCAAATAAACGCCGCCATACCCCGCCAGACGACTCGCATGCTGGCCCATTGGGCCATCCAGCCCAGCCGGCACTAcc                                                                                                                                     >  1:147184/1‑148 (MQ=255)

         aTCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGc                                                                                                                               >  2:42043/1‑151 (MQ=255)

                 gACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAAt                                                                                                                       <  1:171609/151‑1 (MQ=255)

                 gACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAAt                                                                                                                       >  1:452373/1‑151 (MQ=255)

                     aaTACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTccc                                                                                                                   >  1:525896/1‑151 (MQ=255)

                      aTACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCt                                                                                                                  <  1:613918/151‑1 (MQ=255)

                                    tACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAAc                                                                                                      <  2:85893/149‑1 (MQ=255)

                                     aCCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACggg                                                                                                   >  2:386201/1‑151 (MQ=255)

                                           aTGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCgg                                                                                              >  2:208767/1‑150 (MQ=255)

                                                 aTGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTg                                                                                       <  1:407867/151‑1 (MQ=255)

                                                       aaaTCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTg                                                                                 >  2:440238/1‑151 (MQ=255)

                                                        aaTCGGCGTTCGTACCATGAAGCAAATAATCGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGtt                                                                                  <  1:653203/149‑1 (MQ=255)

                                                           cGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGc                                                                               <  2:520233/149‑1 (MQ=255)

                                                           cGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCg                                                                              <  2:153854/150‑1 (MQ=255)

                                                             gCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTcc                                                                           <  1:629042/151‑1 (MQ=255)

                                                                 tCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGt                                                                       >  2:562845/1‑151 (MQ=255)

                                                                 tCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGt                                                                       >  2:272364/1‑151 (MQ=255)

                                                                   gTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTg                                                                     <  2:338552/151‑1 (MQ=255)

                                                                     aCCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTggg                                                                   >  2:453554/1‑151 (MQ=255)

                                                                           aaGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGc                                                              >  2:178487/1‑150 (MQ=255)

                                                                               aaaTAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGtt                                                         <  2:349776/151‑1 (MQ=255)

                                                                                    aaCGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCt                                                    <  2:124374/151‑1 (MQ=255)

                                                                                    aaCGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCt                                                    >  1:680827/1‑151 (MQ=255)

                                                                                    aaCGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCt                                                    <  1:594510/151‑1 (MQ=255)

                                                                                       gccgccATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCt                                                    <  2:625685/148‑1 (MQ=255)

                                                                                       gccgccATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCt                                                    >  1:625654/1‑148 (MQ=255)

                                                                                        ccgccATAGGCAGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGaa                                                 <  1:438979/150‑1 (MQ=255)

                                                                                          gccATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACg                                              <  2:84507/151‑1 (MQ=255)

                                                                                           ccATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACgg                                             >  1:478528/1‑151 (MQ=255)

                                                                                                 gccgccAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCa                                       <  2:573795/151‑1 (MQ=255)

                                                                                                          cGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCg                              <  2:332118/151‑1 (MQ=255)

                                                                                                            aaTCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGgt                            <  2:430780/151‑1 (MQ=255)

                                                                                                                       ggCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCgtgt                  <  2:453539/150‑1 (MQ=255)

                                                                                                                        gctcATTTGGTTATCCAGCCGAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCgtgt                  <  1:119837/146‑1 (MQ=255)

                                                                                                                                      ccagcccagcCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGAcc  >  2:155115/1‑151 (MQ=255)

GATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACC  >  NC_000913/1169700‑1169984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A