Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,957,284	A→G	V534A (GTG→GCG)	ptrA ←	protease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,957,284	0	A	G	100.0%	159.1 / NA	46	V534A (GTG→GCG)	ptrA	protease III
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/28); total (18/28)

AACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCGAAAG  >  NC_000913/2957137‑2957431

aaCTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCgcgc                                                                                                                                                  <  1:581844/151‑1 (MQ=255)

   tGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCgcgcgc                                                                                                                                                >  2:328064/1‑150 (MQ=255)

               aGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACtcat                                                                                                                                   <  1:239913/151‑1 (MQ=255)

                gCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACtcatc                                                                                                                                  <  1:89823/151‑1 (MQ=255)

                     gCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATcaacaa                                                                                                                             <  2:279685/151‑1 (MQ=255)

                             aTCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGctct                                                                                                                     >  1:547932/1‑151 (MQ=255)

                               cATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTgg                                                                                                                   >  2:27208/1‑151 (MQ=255)

                               cATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTgg                                                                                                                   <  2:189175/151‑1 (MQ=255)

                                     gcgcAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTc                                                                                                             <  2:219142/151‑1 (MQ=255)

                                       gcAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGt                                                                                                           >  2:196432/1‑151 (MQ=255)

                                        cAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGt                                                                                                           >  2:386405/1‑150 (MQ=255)

                                             aTCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATttct                                                                                                     >  2:32742/1‑151 (MQ=255)

                                             aTCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATttct                                                                                                     <  1:612542/151‑1 (MQ=255)

                                                    gATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTg                                                                                               <  1:208418/150‑1 (MQ=255)

                                                          gggCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAAt                                                                                        <  2:683232/151‑1 (MQ=255)

                                                             cgcTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAg                                                                                     <  2:463009/151‑1 (MQ=255)

                                                              gcTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGc                                                                                    <  2:224704/151‑1 (MQ=255)

                                                                   ccATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCgaga                                                                                >  2:414650/1‑150 (MQ=255)

                                                                        gCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAatcat                                                                          <  2:636285/151‑1 (MQ=255)

                                                                           ttCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAAtcatca                                                                        <  2:301449/150‑1 (MQ=255)

                                                                             cGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGa                                                                     <  2:417764/151‑1 (MQ=255)

                                                                              ggATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGaa                                                                    <  1:546687/151‑1 (MQ=255)

                                                                                     gCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAgg                                                             >  1:407319/1‑151 (MQ=255)

                                                                                       aaaaTCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGGATATAAggg                                                            >  1:370289/1‑150 (MQ=255)

                                                                                            cAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGc                                                      >  1:609824/1‑151 (MQ=255)

                                                                                             aGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGct                                                     <  2:644863/151‑1 (MQ=255)

                                                                                                cTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTg                                                  >  1:547472/1‑151 (MQ=255)

                                                                                                 tGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTgg                                                 >  2:358423/1‑151 (MQ=255)

                                                                                                  gACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATAAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTg                                                  <  1:8021/149‑1 (MQ=255)

                                                                                                    cATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCaa                                              <  2:675734/151‑1 (MQ=255)

                                                                                                     aTCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCaaa                                             <  1:137383/151‑1 (MQ=255)

                                                                                                            ttGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAagagag                                        <  2:385829/149‑1 (MQ=255)

                                                                                                              gggCTCGCTGGCAAAATAACGGCTTGGCGCATACCCCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAgcgc                                     <  1:386392/150‑1 (MQ=255)

                                                                                                              gggCTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCa                                    <  2:218987/151‑1 (MQ=255)

                                                                                                                 cTCGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAAt                                  >  1:121661/1‑150 (MQ=255)

                                                                                                                   cGCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATg                                 <  1:470559/149‑1 (MQ=255)

                                                                                                                    gCTGGCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTcg                              >  2:530743/1‑151 (MQ=255)

                                                                                                                       ggCAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTcggc                            <  1:358412/150‑1 (MQ=255)

                                                                                                                         cAAAATAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCggcggc                         >  1:637895/1‑151 (MQ=255)

                                                                                                                          aaaaTAACGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCt                        <  1:51599/151‑1 (MQ=255)

                                                                                                                           aaaTAACGGCTTGGAGCATACACCGCGAGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCtt                       >  2:584488/1‑151 (MQ=255)

                                                                                                                                 cGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCt                 <  2:547958/151‑1 (MQ=255)

                                                                                                                                 cGGCTTGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCt                 >  2:628696/1‑151 (MQ=255)

                                                                                                                                     ttGGCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGcc              >  1:576457/1‑150 (MQ=255)

                                                                                                                                       ggCGCATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCa             <  1:533499/149‑1 (MQ=255)

                                                                                                                                          gcATACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCgg        <  2:513104/151‑1 (MQ=255)

                                                                                                                                            aTACACCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCg      <  1:480098/151‑1 (MQ=255)

                                                                                                                                              acacCGCGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCg      >  2:346694/1‑149 (MQ=255)

                                                                                                                                               cacctcGCGCAGATTCGACTCATCAACAAGCAGCTCTGGATGGTCGTAGTTCTTCGCTGACATAATCAGCGAGAAAGCTTCAGGAATTTAAGGGTTCAGCTCTGGCAAAGAGAGCTCAATGTCGGCTGCTTTTTTCTGCCAGTCGGCGaaa   <  1:98830/151‑1 (MQ=255)

                                                                                                                                                accgcGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCGAAAg  <  1:315203/151‑1 (MQ=255)

AACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCGAAAG  >  NC_000913/2957137‑2957431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A