Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,012,795	T→C	N482S (AAC→AGC)	yqeB ←	XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,012,795	0	T	C	100.0%	181.9 / NA	48	N482S (AAC→AGC)	yqeB	XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/24); total (24/24)

GCCCTGATGCATCAACATCATTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGAGCACGGATCACCCGTC  >  NC_000913/3012650‑3012939

gCCCTGATGCATCAACATCATTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTcaa                                                                                                                                              >  2:13148/1‑150 (MQ=255)

   cTGATGCATCAACATCATTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCGGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTcaaca                                                                                                                                            >  1:641749/1‑149 (MQ=255)

          aTCAACATCATTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGc                                                                                                                                    >  2:40413/1‑150 (MQ=255)

              aCATCATTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCAt                                                                                                                               >  2:229549/1‑151 (MQ=255)

              aCATCATTAACGCCTCAAGCACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCAt                                                                                                                               >  1:529320/1‑151 (MQ=255)

                atcatTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATcc                                                                                                                             >  2:501254/1‑151 (MQ=255)

                  catTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATcccc                                                                                                                           <  1:686602/151‑1 (MQ=255)

                  catTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATccc                                                                                                                            <  2:210799/150‑1 (MQ=255)

                   atTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCg                                                                                                                          >  1:80501/1‑151 (MQ=255)

                      aaCGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTc                                                                                                                        <  2:673266/150‑1 (MQ=255)

                      aaCGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTc                                                                                                                        <  1:398682/150‑1 (MQ=255)

                       aCGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCaa                                                                                                                      <  2:401123/151‑1 (MQ=255)

                          ccTCAAGTACGCCGCCGCCAATCGCCCGGACTTTATCAGAAACGCGCGTGAAATCAGCGGTTTCACCAAGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGACGTCGCTCAACAAGCCACGCCCCATGCCCGTCAACg                                                                                                                    <  1:632962/150‑1 (MQ=255)

                                 tACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCttt                                                                                                             >  2:342425/1‑150 (MQ=255)

                                  aCGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTa                                                                                                            <  2:575668/150‑1 (MQ=255)

                                            cAATCGCCCGGCCTTTGTCAGAAACGCTGGTGAAATCAGCGGTTTCACCCCGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCGCAACAACCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTc                                                                                                 >  1:173417/1‑151 (MQ=255)

                                                cGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCa                                                                                             <  2:500126/151‑1 (MQ=255)

                                                cGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCa                                                                                             <  1:209009/151‑1 (MQ=255)

                                                  cccGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATACCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAAt                                                                                           >  2:207887/1‑151 (MQ=255)

                                                    cGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATcc                                                                                         >  2:356447/1‑151 (MQ=255)

                                                               aGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATcac                                                                               >  1:48319/1‑150 (MQ=255)

                                                                gAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATcaca                                                                              >  1:153707/1‑150 (MQ=255)

                                                                 aaaCGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATc                                                                            <  1:599699/151‑1 (MQ=255)

                                                                        ggTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCctc                                                                      <  1:601856/150‑1 (MQ=255)

                                                                         gTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCtt                                                                    >  2:106539/1‑151 (MQ=255)

                                                                           gAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCttt                                                                   <  1:67050/150‑1 (MQ=255)

                                                                           gAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCttt                                                                   <  1:241634/150‑1 (MQ=255)

                                                                              aTCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCAcc                                                               <  1:421861/151‑1 (MQ=255)

                                                                               tCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCAcc                                                               >  2:342351/1‑150 (MQ=255)

                                                                                   cGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCAcc                                                               >  2:285687/1‑146 (MQ=255)

                                                                                            cacGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAttt                                                 <  2:186365/151‑1 (MQ=255)

                                                                                             acGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTc                                                <  1:661902/151‑1 (MQ=255)

                                                                                               gAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCACCTAATTTCAc                                              <  2:345431/151‑1 (MQ=255)

                                                                                                aGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACg                                             <  2:91427/151‑1 (MQ=255)

                                                                                                      gATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGAt                                       <  1:361839/151‑1 (MQ=255)

                                                                                                        tatCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCg                                     >  1:398979/1‑151 (MQ=255)

                                                                                                         atCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGc                                    <  1:489934/151‑1 (MQ=255)

                                                                                                            aCCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATa                                 <  2:653245/151‑1 (MQ=255)

                                                                                                                 ttttAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGcc                            >  2:368185/1‑151 (MQ=255)

                                                                                                                  tttAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCt                           >  2:236253/1‑151 (MQ=255)

                                                                                                                     aaaaCCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTgc                         >  2:83232/1‑150 (MQ=255)

                                                                                                                        accaccAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTgcagca                     <  1:47225/151‑1 (MQ=255)

                                                                                                                            ccaaccaCTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGa                  <  2:452252/150‑1 (MQ=255)

                                                                                                                             caaccaCTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGAGc                >  1:344521/1‑151 (MQ=255)

                                                                                                                                     gCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGAGCACGGATc         >  2:499995/1‑150 (MQ=255)

                                                                                                                                      ccAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGAGCACGGATCa        >  1:305859/1‑150 (MQ=255)

                                                                                                                                         ggCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGAGCACGGATCACCCg    >  1:593598/1‑151 (MQ=255)

                                                                                                                                           ccGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGAGCACGGATCACCCGTc  <  1:491770/151‑1 (MQ=255)

GCCCTGATGCATCAACATCATTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGAGCACGGATCACCCGTC  >  NC_000913/3012650‑3012939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A