Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,031,161	(A)_6→7	coding (1/1332 nt)	pepQ →	proline dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,031,155	1	.	A	100.0%	194.5 / NA	48	intergenic (‑184/‑6)	fadB/pepQ	fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase/proline dipeptidase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (27/21); total (27/21)

GTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGT‑AAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTTCTCGACGATCATCC  >  NC_000913/4031005‑4031300

gTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGt                                                                                                                                                    <  1:463148/151‑1 (MQ=255)

      gATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGT‑aaaaaa                                                                                                                                             <  2:324140/151‑1 (MQ=255)

      gATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGT‑aaaaaa                                                                                                                                             <  2:244200/151‑1 (MQ=255)

                 gtgtgtATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACt                                                                                                                                   >  2:426151/1‑151 (MQ=255)

                 gtgtgtATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACt                                                                                                                                   >  2:505264/1‑151 (MQ=255)

                       aTTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCt                                                                                                                              <  2:280966/150‑1 (MQ=255)

                       aTTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTc                                                                                                                             >  2:467036/1‑151 (MQ=255)

                           tgtgTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGctc                                                                                                                         >  2:517216/1‑151 (MQ=255)

                           tgtgTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGctc                                                                                                                         >  2:536864/1‑151 (MQ=255)

                              gtTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCta                                                                                                                       >  1:471282/1‑150 (MQ=255)

                                tAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCtat                                                                                                                      >  1:188788/1‑149 (MQ=255)

                                 aaaaaTATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATaaa                                                                                                                   <  1:591726/151‑1 (MQ=255)

                                 aaaaaTATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATaa                                                                                                                    <  2:473105/150‑1 (MQ=255)

                                 aaaaaTATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATaa                                                                                                                    >  1:205110/1‑150 (MQ=255)

                                   aaaTATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATaaaaa                                                                                                                 <  1:145291/151‑1 (MQ=255)

                                   aaaTATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATaaaa                                                                                                                  >  2:464157/1‑150 (MQ=255)

                                       atGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCa                                                                                                              <  1:567989/150‑1 (MQ=255)

                                           aaacaaaAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCatat                                                                                                           >  2:589843/1‑149 (MQ=255)

                                           aaacaaaAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAg                                                                                                         <  1:464163/151‑1 (MQ=255)

                                               aaaaGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCGCGCTCTATAAAAATCATATAGCTa                                                                                                      <  1:26719/150‑1 (MQ=255)

                                                      tAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACa                                                                                               <  2:492025/150‑1 (MQ=255)

                                                      tAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACa                                                                                               <  1:51071/150‑1 (MQ=255)

                                                              tGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGAc                                                                                      >  1:47297/1‑151 (MQ=255)

                                                              tGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGAc                                                                                      >  1:280672/1‑151 (MQ=255)

                                                                ccGCTCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTc                                                                                    >  1:499909/1‑151 (MQ=255)

                                                                 cGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTcg                                                                                   >  2:164120/1‑151 (MQ=255)

                                                                  gATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTc                                                                                    >  1:378941/1‑149 (MQ=255)

                                                                     cTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGAt                                                                               >  2:639216/1‑151 (MQ=255)

                                                                       gATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATgc                                                                             >  1:204840/1‑151 (MQ=255)

                                                                          cACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATgcgc                                                                           <  1:490671/150‑1 (MQ=255)

                                                                           aCGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTg                                                                         <  2:94292/151‑1 (MQ=255)

                                                                            cGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTgg                                                                        >  2:564282/1‑151 (MQ=255)

                                                                               tCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGgcgc                                                                     >  2:26200/1‑151 (MQ=255)

                                                                                   cAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCtt                                                                 >  1:3262/1‑151 (MQ=255)

                                                                                             gCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGAt                                                       >  1:44634/1‑151 (MQ=255)

                                                                                               tctGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGc                                                     >  1:439617/1‑151 (MQ=255)

                                                                                                ctGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATg                                                      <  2:223254/149‑1 (MQ=255)

                                                                                                  ggATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGtt                                                  <  1:257080/151‑1 (MQ=255)

                                                                                                      gAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACtt                                              >  2:9482/1‑151 (MQ=255)

                                                                                                      gAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACt                                               <  1:427258/150‑1 (MQ=255)

                                                                                                            tgtgCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCAc                                        >  1:146723/1‑151 (MQ=255)

                                                                                                                    gATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGa                                <  2:683941/151‑1 (MQ=255)

                                                                                                                       gCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCt                             <  1:235855/151‑1 (MQ=255)

                                                                                                                        cgtagaCTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCATCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTg                            >  1:339861/4‑151 (MQ=255)

                                                                                                                              cTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAAt                      >  2:440122/1‑151 (MQ=255)

                                                                                                                                tAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATg                     <  2:227198/150‑1 (MQ=255)

                                                                                                                                 aaGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGtt                   <  2:47297/151‑1 (MQ=255)

                                                                                                                                     tCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTtc               >  2:519005/1‑151 (MQ=255)

                                                                                                                                        aaaaaaCAGAAGGTTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGTATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTtctc             <  1:173113/150‑1 (MQ=255)

                                                                                                                                        aaaaaaCAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTTCTcg            <  1:492157/151‑1 (MQ=255)

                                                                                                                                                  aGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTTCTCGACGATCATcc  <  1:539123/151‑1 (MQ=255)

GTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGT‑AAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTTCTCGACGATCATCC  >  NC_000913/4031005‑4031300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A